In vitro Micropropagation of a Local Oil-Type Olive Genotype (Olea europaea L.) from Kahramanmaraş

Öz

Olive tree (Olea europaea L.) is one of the oldest known and cultivated trees in the world. It is cultivated in many regions owing to its high economic value, high nutrient and oil content, drought resistance, and ability to produce fruit for many years. However, since vegetative propagation, particularly by cuttings, is difficult for many olive cultivars, classical propagation methods remain limited. In this study, in vitro micropropagation of a local oil-type olive genotype grown in the Kahramanmaraş region was investigated using explants of that genotype. Explants were first surface-sterilized and then transferred to ½ MS medium. Shoots proliferating on this medium were subsequently transferred to a regeneration medium to determine the most suitable medium for propagation. Four different media (MS, DKW, OM, and WPM), each supplemented with 1 mg/L of the plant growth regulator BAP (6-benzylaminopurine), were tested for shoot regeneration. Rooting trials were then established on the best-performing regeneration medium, to which different concentrations of IBA (0.5, 1.0, and 1.5 mg/L) were added. The best surface sterilization result was obtained with 15% NaOCl combined with a 2 h chlorine dioxide pre-treatment, giving the highest viability (88.9%). Among the four media tested, WPM supplemented with 1 mg/L BAP gave the best results (shoot length: 16.39 mm; callus formation: 100%), and 1.0 mg/L IBA gave the most effective rooting response among the concentrations tested (shoot length: 15.58 mm).

Anahtar Kelimeler

• Olive
• Tissue culture
• Micropropagation
• Olea europaea
• WPM

Etik Beyan

Ethics committee approval was not required for this study because of there was no study on animals or humans.

Teşekkür

This article was derived from the master's thesis of Osman Yiğit, entitled "In vitro Micropropagation of a Local Oil-Type Olive Genotype (Olea europaea L.) from Kahramanmaraş," prepared under the supervision of Adem Bardak at the Graduate School of Natural and Applied Sciences, Kahramanmaraş Sütçü İmam University.

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