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The Protective Effect Mechanisms of Polyenylphosphatidylcholine PPC Pretreatment Against Stress Induced Ulcer in Rats

Year 2018, , 1 - 9, 01.01.2018
https://doi.org/10.5505/abantmedj.2018.82150

Abstract

INTRODUCTION: This study aims to investigate the mechanisms that are involved in protective actions exerted by pretreatment with polyenylphosphatidylcholine PPC against experimentally induced stress ulcer in rats.METHODS: Forty Swiss albino rats were divided into three groups. Group 1 n=10 was control, group 2 n=15 was stress ulcer and group 3 n=15 was PPC treated rats with stress ulcer. PPC treatment was started ten days before stress at a dose of 100 mg/day by oral route. Rats were terminated, stomachs were excised. Macroscopic ulcer index UI , production of reactive oxygen species, lipid peroxidation, plasma PGE2 and LTC4 levels, fatty-acid compositions were studied. The specimens were examined histopathologically.RESULTS: UI was significantly lower in the treatment group compared with non-treatment group. PGE2 decreased p=0.00 during cold restraint stress. PPC pretreatment was observed to inhibit the decrease in PGE2 in the induced stress ulcer. In the PPC treatment group LTC4 levels were significantly lower than non-treatment group p=0.00 . Additionally, PPC pretreatment inhibited degranülation of the submucosal mast cells. The inhibitory effect of PPC was more potent on red- stained mast cells than on blue-stained cells.DISCUSSION AND CONCLUSION: The present study shows that protective effects of PPC could be ascribed to inhibition of mast cell degranulation, a reduction in gastric oxidative injury, increase in biosynthesis of protective PGE2, and a decrease in biosynthesis of LTC4.

References

  • Haglund U. Stress ulcers. Scand J Gastroenterol Suppl.1990; 175: 27-33.
  • Fennerty MB. Pathophysiology of the upper gastrointestinal tract in the critically ill patient: rationale for the therapeutic benefits of acid suppression. Crit Care Med. Jun; 2002; 30: 351-5.
  • Stein TA, Keegan LM, Auguste LJ, et al: Stress-induced gastric lesions and the synthesis of prostoglandis, and leukotriens. J Surg Res 1991; 51: 368-371.
  • Kwiecien S, Brzozowski T, Konturek SJ: Effects of reactive oxygen species action on gastric mucosa in various models of mucosal injury. J Physiol Pharmacol. 2002; 53: 39- 50
  • Kwiecień S, Brzozowski T, Konturek PCh, Konturek SJ: The role of reactive oxygen species in action of nitric oxide- donors on stress-induced gastric mucosal lesions. J Physiol Pharmacol . 2002;53:761-73.
  • Demirbilek et al. Protective Effect of Polyunsatured Phosphatidylcholine Pretreatment on Stres Formation in Rats. Journal of Pedatric Surgery, 2004; 39: 57-62.
  • Das D, Banerjee RK: Effect of stress on the antioxidant enzymes and gastric ulceration. Mol Cell Biochem 1993;125:1115-1125.
  • Tuncel N, Tuncel M,. Aboul-Enein HY: Effects of the vasoactive intestinal peptide on stress-induced mucosal ulcers and modulation of methylation of histamine in gastric tissue of the rats. Il Farmaco 2003; 58: 449-454.
  • Cho CH, Ogle CW: Cholinergic-mediated gastric mast cell degranulation with subsequent histamine H1- and H2- receptor activation in stress ulceration in rats. Eur J Pharmacol 1979; 55 : 23-33.
  • Uchiyoma M, Mihara M: Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem 1978; 36: 271-278.
  • Prajda N, Weber G. Malignant transformation-linked imbalance: decreased XO activity in hepatomas. FEBS Lett 1975; 59: 245–249.
  • Green LC, Wagner DA, Glogowski J, et al: Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids. Anal Biochem 126: 131-138,1982
  • Aebi H: Catalase. In: Methods of Enzymatic Analysis. Academic Press: New York: 1974; 673–67.
  • Beutler, E: Glutathione in red blood cell metabolism. A Manuel of Biochemical Methods, 1975.
  • Sun Y, Oberley LW, Li Y: A simple method for clinical assay of superoxide dismutase. Clin. Chem. 1988; 34: 497–500.
  • Goswami S, Mai J, Bruckner G, Kinsella J.E. Extraction and purification of prostaglandins and thromboxane from biological samples for gas chromatographic analysis. Prostaglandins 1981; 225:693-702.
  • Hara A, Radin NS. Lipid extraction of tissues with a low- toxicity solvent. Anal Biochem. 1978; 90: 420-6.
  • Tanaka R, Fujisawa S, Kawamura K, Harada M. Paraquat- induced enhancement of vascular permeability. J Toxicol Sci.1983; 8:147-59.
  • Crivellato E, Finato N, Isola M, Ribatti D, Beltrami CA: Low mast cell density in the human duodenal mucosa from chronic inflammatory duodenal bowel disorders is associated with defective villous architecture. Eur J Clin Invest.2003; 33:601-10.
  • Stassen M, Hultner L, Schmitt E: Classical and alternative pathways of mast cell activation. Crit Rev Immunol. 2002; 22:115-40.
  • Khadzhiev OC, Lupal'tsov VI, Simonenkov AP, Klimenko NA, Tatarko SV: Microcirculatory disturbances in gastric mucosa during ulcer disease and effects of serotonin on their dynamics. Bull Exp Biol Med.2000 ;130: 843-5.
  • Nishida K, Ohta Y, Kobayashi T, Ishiguro I: Involvement of the xanthine-xanthine oxidase system and neutrophils in the development of acute gastric mucosal lesions in rats with water immersion restraint stress. Digestion. 1997;58: 340-51.
  • Aleynik SI, Leo MA, Takeshige U, Aleynik MK, Lieber CS. Dilinoleoylphosphatidylcholine is the active antioxidant of polyenylphosphatidylcholine. Investig Med. 1999; 47: 507- 12.
  • Takeuchi K, Suzuki K, Araki H, Mizoguchi H, Sugamoto S, Umdeda M. Roles of endogenous prostaglandins and nitric oxide in gastroduodenal ulcerogenic responses induced in rats by hypothermic stress. J Physiol Paris. 1999; 93: 423- 31.
  • Brzozowski T, Konturek PC, Konturek SJ, Drozdowicz D, Pajdo R, Pawlik M, Brzozowska I, Hahn EG. Expression of cyclooxygenase (COX)-1 and COX-2 in adaptive cytoprotection induced by mild stress. J Physiol Paris. 2000; 94: 83-91.

Poliansature Fosfotidilkolinin PFK Stres Ülser Gelişimini Engellemedeki Etkinlik Mekanizmaları

Year 2018, , 1 - 9, 01.01.2018
https://doi.org/10.5505/abantmedj.2018.82150

Abstract

GİRİŞ ve AMAÇ: PPC ile prolifilaksinin, stres ülser gelişimini engellemede etkin olduğu daha önce yapılan deneysel bir çalışmada gösterilmiştir. Yine rat stres ülser modelinde gerçekleştirilen bu deneysel çalışmada, bu etkinliğin altında yatan mekanizmaların araştırılması amaçlanmıştır.YÖNTEM ve GEREÇLER: Ratlar, Grup1 n=10 kontrol, Grup2 n=15 stres ülser ve Grup3 n=15 PFK tedavisi + stres ülser olmak üzere üç gruba ayrıldı. Stres ülser modeli için Grup 2 ve grup 3 ratlar, 72 saatlik açlık periyodundan sonra, immobilize halde, +4 C?de, 4 saat soğuğa maruz bırakıldı. Grup 3 ratlara, PFK 100 mg/gün dozunda, oral yoldan, deney öncesi 10 gün süreyle verildi. Deney periyodu sonunda total çıkartılan ve büyük kurvatur boyunca açılan midelerde okülometrik yöntemle ülser indeksi UI hesaplandı Gastrik doku örneklerinde malondialdehit MDA ve superoksit dismutaz SOD , glutatyon GSH , ksantin oksidaz XO , eritrositlerde katalaz CAT , plazma örneklerinde ise total nitrit+nitrat, prostaglandin E2 PGE2 ve lökotrien C4 LTC4 düzeyleri ölçüldü. Histopatolojik incelemede gastrik mukozal hasar H&E boyama ve mast hücre degranülasyonu dominici boyama değerlendirildi.BULGULAR: Ülser indeksi UI ' nin PFK ile profilaksi grubunda, grup 2'den anlamlı düzeyde düşük olduğu görüldü p

References

  • Haglund U. Stress ulcers. Scand J Gastroenterol Suppl.1990; 175: 27-33.
  • Fennerty MB. Pathophysiology of the upper gastrointestinal tract in the critically ill patient: rationale for the therapeutic benefits of acid suppression. Crit Care Med. Jun; 2002; 30: 351-5.
  • Stein TA, Keegan LM, Auguste LJ, et al: Stress-induced gastric lesions and the synthesis of prostoglandis, and leukotriens. J Surg Res 1991; 51: 368-371.
  • Kwiecien S, Brzozowski T, Konturek SJ: Effects of reactive oxygen species action on gastric mucosa in various models of mucosal injury. J Physiol Pharmacol. 2002; 53: 39- 50
  • Kwiecień S, Brzozowski T, Konturek PCh, Konturek SJ: The role of reactive oxygen species in action of nitric oxide- donors on stress-induced gastric mucosal lesions. J Physiol Pharmacol . 2002;53:761-73.
  • Demirbilek et al. Protective Effect of Polyunsatured Phosphatidylcholine Pretreatment on Stres Formation in Rats. Journal of Pedatric Surgery, 2004; 39: 57-62.
  • Das D, Banerjee RK: Effect of stress on the antioxidant enzymes and gastric ulceration. Mol Cell Biochem 1993;125:1115-1125.
  • Tuncel N, Tuncel M,. Aboul-Enein HY: Effects of the vasoactive intestinal peptide on stress-induced mucosal ulcers and modulation of methylation of histamine in gastric tissue of the rats. Il Farmaco 2003; 58: 449-454.
  • Cho CH, Ogle CW: Cholinergic-mediated gastric mast cell degranulation with subsequent histamine H1- and H2- receptor activation in stress ulceration in rats. Eur J Pharmacol 1979; 55 : 23-33.
  • Uchiyoma M, Mihara M: Determination of malonaldehyde precursor in tissues by thiobarbituric acid test. Anal Biochem 1978; 36: 271-278.
  • Prajda N, Weber G. Malignant transformation-linked imbalance: decreased XO activity in hepatomas. FEBS Lett 1975; 59: 245–249.
  • Green LC, Wagner DA, Glogowski J, et al: Analysis of nitrate, nitrite, and [15N] nitrate in biological fluids. Anal Biochem 126: 131-138,1982
  • Aebi H: Catalase. In: Methods of Enzymatic Analysis. Academic Press: New York: 1974; 673–67.
  • Beutler, E: Glutathione in red blood cell metabolism. A Manuel of Biochemical Methods, 1975.
  • Sun Y, Oberley LW, Li Y: A simple method for clinical assay of superoxide dismutase. Clin. Chem. 1988; 34: 497–500.
  • Goswami S, Mai J, Bruckner G, Kinsella J.E. Extraction and purification of prostaglandins and thromboxane from biological samples for gas chromatographic analysis. Prostaglandins 1981; 225:693-702.
  • Hara A, Radin NS. Lipid extraction of tissues with a low- toxicity solvent. Anal Biochem. 1978; 90: 420-6.
  • Tanaka R, Fujisawa S, Kawamura K, Harada M. Paraquat- induced enhancement of vascular permeability. J Toxicol Sci.1983; 8:147-59.
  • Crivellato E, Finato N, Isola M, Ribatti D, Beltrami CA: Low mast cell density in the human duodenal mucosa from chronic inflammatory duodenal bowel disorders is associated with defective villous architecture. Eur J Clin Invest.2003; 33:601-10.
  • Stassen M, Hultner L, Schmitt E: Classical and alternative pathways of mast cell activation. Crit Rev Immunol. 2002; 22:115-40.
  • Khadzhiev OC, Lupal'tsov VI, Simonenkov AP, Klimenko NA, Tatarko SV: Microcirculatory disturbances in gastric mucosa during ulcer disease and effects of serotonin on their dynamics. Bull Exp Biol Med.2000 ;130: 843-5.
  • Nishida K, Ohta Y, Kobayashi T, Ishiguro I: Involvement of the xanthine-xanthine oxidase system and neutrophils in the development of acute gastric mucosal lesions in rats with water immersion restraint stress. Digestion. 1997;58: 340-51.
  • Aleynik SI, Leo MA, Takeshige U, Aleynik MK, Lieber CS. Dilinoleoylphosphatidylcholine is the active antioxidant of polyenylphosphatidylcholine. Investig Med. 1999; 47: 507- 12.
  • Takeuchi K, Suzuki K, Araki H, Mizoguchi H, Sugamoto S, Umdeda M. Roles of endogenous prostaglandins and nitric oxide in gastroduodenal ulcerogenic responses induced in rats by hypothermic stress. J Physiol Paris. 1999; 93: 423- 31.
  • Brzozowski T, Konturek PC, Konturek SJ, Drozdowicz D, Pajdo R, Pawlik M, Brzozowska I, Hahn EG. Expression of cyclooxygenase (COX)-1 and COX-2 in adaptive cytoprotection induced by mild stress. J Physiol Paris. 2000; 94: 83-91.
There are 25 citations in total.

Details

Primary Language Turkish
Journal Section Research Article
Authors

Necla Gürbüz Sarıkaş This is me

Melih Akın This is me

Kubilay Gürünlüoğlu This is me

Erkan Taş This is me

Aysun Bay Karabulut This is me

İclal Gürses This is me

Publication Date January 1, 2018
Published in Issue Year 2018

Cite

APA Gürbüz Sarıkaş, N., Akın, M., Gürünlüoğlu, K., Taş, E., et al. (2018). Poliansature Fosfotidilkolinin PFK Stres Ülser Gelişimini Engellemedeki Etkinlik Mekanizmaları. Abant Medical Journal, 7(1), 1-9. https://doi.org/10.5505/abantmedj.2018.82150
AMA Gürbüz Sarıkaş N, Akın M, Gürünlüoğlu K, Taş E, Karabulut AB, Gürses İ. Poliansature Fosfotidilkolinin PFK Stres Ülser Gelişimini Engellemedeki Etkinlik Mekanizmaları. Abant Med J. January 2018;7(1):1-9. doi:10.5505/abantmedj.2018.82150
Chicago Gürbüz Sarıkaş, Necla, Melih Akın, Kubilay Gürünlüoğlu, Erkan Taş, Aysun Bay Karabulut, and İclal Gürses. “Poliansature Fosfotidilkolinin PFK Stres Ülser Gelişimini Engellemedeki Etkinlik Mekanizmaları”. Abant Medical Journal 7, no. 1 (January 2018): 1-9. https://doi.org/10.5505/abantmedj.2018.82150.
EndNote Gürbüz Sarıkaş N, Akın M, Gürünlüoğlu K, Taş E, Karabulut AB, Gürses İ (January 1, 2018) Poliansature Fosfotidilkolinin PFK Stres Ülser Gelişimini Engellemedeki Etkinlik Mekanizmaları. Abant Medical Journal 7 1 1–9.
IEEE N. Gürbüz Sarıkaş, M. Akın, K. Gürünlüoğlu, E. Taş, A. B. Karabulut, and İ. Gürses, “Poliansature Fosfotidilkolinin PFK Stres Ülser Gelişimini Engellemedeki Etkinlik Mekanizmaları”, Abant Med J, vol. 7, no. 1, pp. 1–9, 2018, doi: 10.5505/abantmedj.2018.82150.
ISNAD Gürbüz Sarıkaş, Necla et al. “Poliansature Fosfotidilkolinin PFK Stres Ülser Gelişimini Engellemedeki Etkinlik Mekanizmaları”. Abant Medical Journal 7/1 (January 2018), 1-9. https://doi.org/10.5505/abantmedj.2018.82150.
JAMA Gürbüz Sarıkaş N, Akın M, Gürünlüoğlu K, Taş E, Karabulut AB, Gürses İ. Poliansature Fosfotidilkolinin PFK Stres Ülser Gelişimini Engellemedeki Etkinlik Mekanizmaları. Abant Med J. 2018;7:1–9.
MLA Gürbüz Sarıkaş, Necla et al. “Poliansature Fosfotidilkolinin PFK Stres Ülser Gelişimini Engellemedeki Etkinlik Mekanizmaları”. Abant Medical Journal, vol. 7, no. 1, 2018, pp. 1-9, doi:10.5505/abantmedj.2018.82150.
Vancouver Gürbüz Sarıkaş N, Akın M, Gürünlüoğlu K, Taş E, Karabulut AB, Gürses İ. Poliansature Fosfotidilkolinin PFK Stres Ülser Gelişimini Engellemedeki Etkinlik Mekanizmaları. Abant Med J. 2018;7(1):1-9.