The Determination of Minimum Inhibitory Concentration of Eighteen Essential Oils with Resasurine method, Against a Group of Yeast and Bacteria

Year 2021, Volume: 10 Issue: 1, 72 - 80, 30.04.2021

Çiğdem Eda Balkan , Ayşe Usanmaz Bozhüyük , Şaban Kordalı , Hayrunnisa Bekis Bozkurt

https://doi.org/10.47493/abantmedj.2021.35

Abstract

INTRODUCTION: Aim of this study is investigate the effect and MIC values of some essential oils, purified by hydrodistillation method, in some of the pathogenic bacteria and fungi.
METHODS: Achillea gypsicola Hub. (Asteraceae), Ruta graveolens L. (Rutaceae), Satureja hortensis L. (Lamiaceae), Thymbra spicata L. (Lamiaceae), Achillea biebersteinii Afan. (Asteraceae), Artemisia santonicum L. (Asteraceae), Foeniculum vulgare Mill. (Umbelliferae), Origanum acutidens (Hand-Mazz.) (Lamiaceae), Thymus fallax Fisch. & C.A. Mey. (Lamiaceae), Inula graveolens L. (Asteraceae), Dorystoechas hastata Boiss. & Heldr. Ex Bentham (Lamiaceae), Crambe orientalis L. (Brassicaceae), Citrus cinensis L. (Rutaceae), Satureja spicigera (C. Koch) Boiss. (Lamiaceae), Lavandula stoechas L. (Lamiaceae), Satureja montana L. (Lamiaceae), Vitex agnus-castus L. (Verbenaceae), Origanum majorana L. (Lamiaceae) (Rutaceae).
RESULTS: In our study oils were tested against four bacteria and three fungi. The minimum inhibitory concentration and minimum bactericidal concentration values of oils on Acinetobacter baumanii ATCC 49139, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 29213, Eescherichia coli ATCC 25923, Candida albicans ATCC 14053, Candida glabrata ATCC 15126, Candida parapsilosis ATCC 22019 microorganisms was found.
DISCUSSION AND CONCLUSION: It is aimed to find MIC values of oils by adding resazurin dye as a redox indicator.

Keywords

Bacteria and Yeasts, Essential oils, REMA, MIC

Supporting Institution

yok

Project Number

yok

Thanks

Special thanks to the identification of plant materials was made by XX, department of biology, faculty of art and science, XX University. The voucher specimens of these plants have been deposited in the herbarium of XX University. Aerial parts of the plants were dried in shade before processing with a grinder and XX from XX University XX department.

Onsekiz Uçucu Yağın Bazı Maya ve Bakterilere Karşı Minimum İnhibitör Konsantrasyonunun Resazurin Yöntemi ile Belirlenmesi

Abstract

GİRİŞ ve AMAÇ: Bu çalışmanın amacı, insan ve hayvanlar açısından önemli bazı patojenik bakteri ve mantarlara karşı, hidrodistillasyon yöntemi ile elde edilen ve GC-MS yöntemi aile ayrıştırılan 18 çeşit uçucu yağın minimum inhibitör konsantrasyon değerlerini araştırmaktır.
YÖNTEM ve GEREÇLER: Achillea gypsicola (Asteraceae), Ruta graveolens L. (Rutaceae), Satureja hortensis L. (Lamiaceae), Thymbra spicata L. (Lamiaceae), Achillea biebersteinii Afan. (Asteraceae), Artemisia santonicum L. (Asteraceae), Foeniculum vulgare. (Umbelliferae), Origanum acutidens (Lamiaceae), Thymus fallax (Lamiaceae), Inula graveolens L. (Asteraceae), Dorystoechas hastata (Lamiaceae), Crambe orientalis L. (Brassicaceae), Narenciye cinensis L. (Rutaceae), Satureja spicigera (Lamiaceae), Lavandula stoechas L. (Lamiaceae), Satureja montana L. (Lamiaceae), Vitex agnus-castus L. (Verbenaceae), Origanum majorana L. (Lamiaceae) .
BULGULAR: Çalışmamızda yağlar dört bakteri ve üç mantara karşı test edildi; Acinetobacter baumanii ATCC 49139, Pseudomonas aeruginosa ATCC 27853, Staphylococcus aureus ATCC 29213, E.coli ATCC 25923, Candida albicans ATCC 14053, Candida glabrata ATCC 15126, Candida parapsilosis ATCC 22019 mikroorganizmalarının yağlara karşı minimum inhibitör konsantrasyonu bulundu. A. gypsicoala, R. graveolens, S. hortensis, T. spicata, O. majorana ve V. agnus iki dilüsyon tekrarında da bulunan en etkili yağlardır.
TARTIŞMA ve SONUÇ: Çalışmamız, bu yağların hem insan hem de hayvan yaralarının antibakteriyel ve antifungal tedavisine ek olarak kullanılmasının faydalı olacağı düşüncesiyle gerçekleştirilmiştir.

Keywords

MIC, REMA, Bakteri ve Mantarlar, Bitkisel Yağlar

Project Number

yok

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