Sıçan frenik sinir hemi-diyaframına resveratrol, kateşin ve epikateşinin etkileri

Öz

Amaç: Resveratrol, kateşin ve epikateşin iskelet kas atrofisini engelleyen, yorgunluk karşıtı etkileri olan ve egzersiz kapasitesini artıran etkileriyle bilinmektedirler. Çalışmamızda, sıçan frenik sinir hemi-diyafram preparatı kullanılarak bu antioksidanların kas kasılmasına etkisi incelenmiştir.

Yöntemler: Kasılma yanıtları sıçan frenik sinir hemidiyafram preparatına, doğrudan, dolaylı ve tetanik uyarılar verilerek araştırıldı.

Bulgular: Resveratrol ve kateşin uygulanması kısa sürede, dolaylı uyarılarla oluşan kasılma yanıtlarını kolaylaştırdı ancak uzun sürede baskıladı. Epikateşin uygulanması sonucunda doğrudan uyarılarla oluşan kasılma yanıtlarındaki azalma (p=0,010), dolaylı uyarılarla oluşan azalmadan  anlamlı olarak daha fazlaydı. Resveratrol ve kateşin tetanik yanıtları etkilemezken, epikateşin anlamlı derecede baskıladı (p=0,005).

Sonuç: Çalışmanın sonuçları, bu antioksidanların nöromusküler kavşakta presinaptik ve postsinaptik etkileri olduğunu göstermektedir. Resveratrol kas kasılmasını doğrudan etkilemekte, kateşin ve epikateşin ise hem doğrudan hem de dolaylı olarak etkilemektedir.

Anahtar Kelimeler

• Resveratrol,kateşin,epikateşin,sıçan,antioksidan

Effects of resveratrol, catechin and epicatechin on rat phrenic nerve hemi-diaphragm

Abstract

Aim: Resveratrol, catechin and epicatechin are known for their preventive effects of skeletal muscle atrophy, anti-fatique effects and enhancement of exercise capacity. In this study, we investigate the effects these antioxidants on muscle contraction by using rat phrenic nerve hemi-diaphragm preparations.

Methods: Twitch responses are investigated by giving direct and indirect and tetanic stimulation to rat phrenic nerve hemi-diaphragm preparations.

Results: Resveratrol and catechin facilitated twitch responses to the indirect stimulus in short duration and suppressed in long duration. The decrease in the responses to the direct stimulation by applying epicatechin (p=0.010) was significantly higher than the responses by giving indirect stimulation. Epicatechin significantly suppressed tetanic responses (p=0.005), but not resveratrol and catechin.

Conclusion: The results of the study show that these antioxidants have presynaptic and postsynaptic effects on neuromuscular junction. Resveratrol effective on muscle contraction via direct stimulus, catechin and epicatechin effects muscle contraction by direct and indirect stimulus.

Keywords

• Resveratrol,catechin,epicatechin,rat,antioxidant

References

1. 1. Bastianetto S, Ménard C, Quirion R. Neuroprotective action of resveratrol. Biochim Biophys Acta. 2015;1852:1195-201.
2. 2. Fremont L. Minireview: Biological effects of resveratrol. Life Scis. 2000;66: 663-73.
3. 3. Pon V, Anandh B, Dongmin L. Green tea catechins and cardiovascular health: An update. Curr Med Chem. 2008;15:1840-50.
4. 4. Hemdan D, Hirasaka K, Nakao R, Kohno S, Kagawa S, Abe T, et al. Polyphenols prevent clinorotation-induced expression of atrogenes in mouse C2C12 skeletal myotubes. J Med Invest. 2009;56:26-32.
5. 5. Nogueira L, Ramirez-Sanchez I, Perkins GA, Murphy A, Taub P, Ceballos G, et al. (–)-Epicatechin enhances fatigue resistance and oxidative capacity in mouse muscle. J Physiol. 2011;589:4615-31.
6. 6. Basu S, Chaudhuri T, Chauhan SPS, Das Gupta AK, Chaudhury L, Vedasiromoni JR. The theaflavin fraction is responsible for the facilitatory effect of black tea at the skeletal myoneural junction. Life Sci. 2005;76:3081-8.
7. 7. Das M, Vedasiromoni JR, Chauhan SPS, Ganguly DK. Effect of green tea (Camelia sinensis) extract on the rat diaphragm. J Ethnopharmacol. 1997;57:197-201.
8. 8. Satoh E, Ishii T, Shimizu Y, Sawamura S, Nishimura M. Black tea extract, thearubigin fraction, counteract the effects of botulinum neurotoxins in mice. Brit J Pharmacol. 2001;132:797-8.

9. 9. Satoh E, Ishii T, Shimizu Y, Sawamura S, Nishimura M. The mechanism underlying the protective effect of the thearubigin fraction of black tea (camellia sinensis) extract against the neuromuscular blocking action of botulinum neurotoxins. Pharmacol Toxicol. 2002;9:199-202.
10. 10. Giniatullin AR, Giniatullin RA. Dual action of hydrogen peroxide on synaptic transmission at the frog neuromuscular junction. J Physiol. 2003;552:283-93.
11. 11. Crosland RD. Action of reactive oxygen species and their antagonists on twitch tension of the rat phrenic nerve-diaphragm. Pharmacol Toxicol. 1995;77:231-7.
12. 12. Ryan MJ, Jackson JR, Hao Y, Williamson CL, Dabkowski ER, Hollander JM, et al. Suppression of oxidative stress by resveratrol after isometric contractions in gastrocnemius muscles of aged mice. J Gerontol A Biol Sci Med Sci. 2010;65:815-31.
13. 13. Stockinger J, Maxwell N, Shapiro D, deCabo R, Valdez G. Caloric restriction mimetics slow aging of neuromuscular synapses and muscle fibers. J Gerontol A Biol Sci Med Sci. 2017;73:21-8.
14. 14. Bulbring E. Observations on the isolated phrenic nerve diaphragm preparation of the rat. Brit J Pharmacol. 1946;1:38-61.
15. 15. Sinha K, Chaudhary G, Gupta YK. Protective effect of resveratrol against oxidative stress in middle cerebral artery occlusion model of stroke in rats. Life Sci. 2002;71:655-65.
16. 16. Murase T, Haramizu S, Shimotoyodome A, Tokimitsu I, Hase T. Green tea extract improves running endurance in mice by stimulating lipid utilization during exercise. Am J Physiol Regul Integr Comp Physiol. 2006;290:1550-6.