Sıçan frenik sinir hemi-diyaframına resveratrol, kateşin ve epikateşinin etkileri

Yıl 2018, , 170 - 173, 30.11.2018

Aslı Zengin Türkmen , Asiye Nurten

https://doi.org/10.25000/acem.442277

Öz

Amaç:
Resveratrol, kateşin ve epikateşin iskelet kas atrofisini engelleyen, yorgunluk
karşıtı etkileri olan ve egzersiz kapasitesini artıran etkileriyle
bilinmektedirler. Çalışmamızda, sıçan frenik sinir hemi-diyafram preparatı
kullanılarak bu antioksidanların kas kasılmasına etkisi incelenmiştir.

Yöntemler:
Kasılma yanıtları sıçan frenik sinir hemidiyafram preparatına, doğrudan,
dolaylı ve tetanik uyarılar verilerek araştırıldı.

Bulgular:
Resveratrol ve kateşin uygulanması kısa sürede, dolaylı uyarılarla oluşan kasılma
yanıtlarını kolaylaştırdı ancak uzun sürede baskıladı. Epikateşin uygulanması
sonucunda doğrudan uyarılarla oluşan kasılma yanıtlarındaki azalma (p=0,010), dolaylı
uyarılarla oluşan azalmadan  anlamlı
olarak daha fazlaydı. Resveratrol ve kateşin tetanik yanıtları etkilemezken,
epikateşin anlamlı derecede baskıladı (p=0,005).

Sonuç: Çalışmanın
sonuçları, bu antioksidanların nöromusküler kavşakta presinaptik ve
postsinaptik etkileri olduğunu göstermektedir. Resveratrol kas kasılmasını doğrudan
etkilemekte, kateşin ve epikateşin ise hem doğrudan hem de dolaylı olarak
etkilemektedir.

Anahtar Kelimeler

Resveratrol, kateşin, epikateşin, sıçan, antioksidan

Effects of resveratrol, catechin and epicatechin on rat phrenic nerve hemi-diaphragm

Öz

Aim:
Resveratrol, catechin and epicatechin are known for their preventive effects of
skeletal muscle atrophy, anti-fatique effects and enhancement of exercise
capacity. In this study, we investigate the effects these antioxidants on
muscle contraction by using rat phrenic nerve hemi-diaphragm preparations.

Methods:
Twitch responses are investigated by giving direct and indirect and tetanic
stimulation to rat phrenic nerve hemi-diaphragm preparations.

Results:
Resveratrol and catechin facilitated twitch responses to the indirect stimulus
in short duration and suppressed in long duration. The decrease in the responses to the direct stimulation by applying epicatechin
(p=0.010) was significantly higher than the responses by giving indirect
stimulation. Epicatechin significantly suppressed tetanic responses (p=0.005), but not resveratrol and catechin.

Conclusion: The results
of the study show that these antioxidants have presynaptic and postsynaptic
effects on neuromuscular junction. Resveratrol effective on muscle contraction
via direct stimulus, catechin and epicatechin effects muscle contraction by
direct and indirect stimulus.

Anahtar Kelimeler

Resveratrol, catechin, epicatechin, rat, antioxidant

Kaynakça

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