Hesperidin triggering apoptosis on neuroblastoma cell

Abstract

Aim: Neuroblastoma accounts for approximately %10 of all pediatric cancers and approximately %15 of cancer related deaths in children. Understanding of the molecular mechanisms which play role in the progress of this cancer type may lead to develop more effective strategies for therapy. Flavanoids are popular subject for this new strategies. Hesperidin is an herbal flavonoid which is found abundantly in citrus that has been studied on several cancer cell lines. For this purpose, it was aimed to investigate is the apoptotic effects of hesperidin on neuroblastoma tumors using SH-SY5Y cell line.

Methods: Hesperidin was performed on SH-SY5Y and MRC-5 cell line by WST-1, Caspase-3 and Annexin V in a time and dose dependent manner.

Results: 2.5 µM hesperidin and 5 µM hesperidin were found the most suitable dosage for neuroblastoma cell line because of the success on decreasing cell proliferation. Hesperidin has resulted with the ability for apoptotic cell death compared with control group [MRC-5 cell line, p<0.05 for all]. 2,5 µM and 5 µM hesperidin concentration for 48h were ended up early apoptotic results as 53.65% for 2.5 µM and 38.90% for 5 µM. There was no significant change on caspase-3 activity.

Conclusions: Our study suggests that hesperidin would be effective against neuroblastoma tumors. We believe with further investigation this study will be helpful for developing new research areas in neuroblastoma tumors.

Keywords

• Hesperidin,neuroblastoma,apoptosis

Nöroblastoma hücrelerinde hesperidinin apoptozu tetiklemesi

Öz

Amaç: Çocukluk çağında rastlanan pediatrik kanserlerin yaklaşık %10’luk bir kısmını oluşturan nöroblastoma, çocuklarda kansere bağlı ölümlerin yaklaşık %15’lik bir bölümünden de sorumludur. Bu kanser türünün ilerlemesi esnasında rol alan moleküler olayların anlaşılması, tedavi açısından daha etkili yöntemlerin ortaya konmasına aracılık edebilecektir. Hesperidin turunçgillerde bolca bulunan bir flavonoiddir. Bu amaçla, literatürde ilk olarak SH-SY5Y hücre hattı ve hesperidinin olası apoptotik etkisini gösteren çalışma özelliği taşımaktadır.

Yöntem: Hesperidinin SH-SY5Y hücre proliferasyonu ve canlılığı üzerinde doz bağımlı etkisi için WST-1, kaspaz enzim aktivitesinin değerlendirilmesi için Kaspaz 3/BCA ve apoptotik değerlendirme için Annexin V analizleri yapılmıştır.

Bulgular: 2,5 µM hesperidin ve 5 µM hesperidin hücre proliferasyonu üzerinde azalma etkisi yarattığından hücre hattında uygulanmak üzere seçilmiştir. Hesperidin kontrol grubuna göre nöroblastoma üzerinde apoptotik hücre ölümüne neden olmuştur [MRC-5 hücre hattı, hepsi için p<0.05]. 2,5 µM hesperidin ve 5 µM hesperidin 48 saatlik inkübasyon sonucu %53,65 2,5 µM için ve %38,90 5 µM için apoptoz ile sonuçlanmıştır. Kaspaz 3 aktivitesinde ise herhangi bir değişim gözlenmemiştir.

Sonuç: Bu çalışmamızda elde ettiğimiz sonuçlardan, nöroblastoma tümörlerinde hesperidinin antikarsinojenik etki gösterdiği izlenimi edinilmiştir. Elde edilen verilerden yola çıkarak, çalışmamızın yapılacak ileri araştırmalar ile birlikte nöroblastoma tümörlerinde yeni araştırma alanlarının oluşmasında katkısı olabileceğini düşünmekteyiz.

Anahtar Kelimeler

• nöroblastoma,hesperidin,apoptoz

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