Analysis of JAM-A T>C (rs790056) and LFA-1 2120 G>C (rs2230433) gene variations in uterine leiomyoma

Year 2021, , 48 - 52, 22.01.2021

Özge Kömürcü Karuserci , Esra Güzel Tanoğlu , Halime Hanım Pençe Mete Gürol Uğur

https://doi.org/10.38053/acmj.843786

Abstract

Aim: We aimed to investigate the effect of junctional adhesion molecule-A (JAM-A) and
lymphocyte function-associated antigen 1 (LFA-1) gene variants on the development of
leiomyoma in Turkish women.
Materials and methods: Retrospectively, leiomyoma tissues from 102 patients who were operated due to leiomyoma between May 2018 and April 2019 and healthy myometrium tissues from 70 control group patients without leiomyoma who underwent hysterectomy due to other reasons were included in the study. JAM-A rs790056 (T>C) and LFA-1 rs2230433 (G>C) gene variants in all tissues were examined by the quantitative real-time polymerase chain reaction (qRT-PCR) technique. Statistical analyses were performed using SPSS 16 software package.
Results: The frequency of JAM-A rs790056 CC genotype and C allele was significantly higher in the leiomyoma group compared to the control group (p=0.01, p=0.02), as well as the LFA-1
rs2230433 GG genotype which was also higher compared to the control group (p=0.01).
Conclusion: JAM-A rs790056 was found to be more effective than LFA-1 rs2230433 in
determining the risk of uterine leiomyoma. According to the results, it has been determined that
variations in JAM-A and LFA-1 genes may cause predisposition to uterine leiomyoma in Turkish women.

Keywords

Leiomyoma, uterus, gene variations, Turkish women

Supporting Institution

University of Health Sciences Scientific Research Projects Unit

Project Number

2018/055

References

• 1. Stewart EA, Cookson CL, Gandolfo RA, Schulze-Rath. Epidemiology of uterine fibroids: a systematic review. BJOG. 2017; 124: 1501-12.
• 2. Rice KE, Secrist JR, Woodrow EL, Hallock LM, Neal JL. Etiology, diagnosis, and management of uterine leiomyomas. J Midwifery Womens Health. 2012; 57: 241-7.
• 3. Wise LA, Ruiz-Narvaez EA, Palmer JR, et al. African ancestry and genetic risk for uterine leiomyomata. Am J Epidemiol, 2012; 176(12): 1159-68.
• 4. Jeon YT, Kim JW, Park NH, Song YS, Kang SB, Lee HP . DNA repair gene XRCC1 Arg399Gln polymorphism is associated with increased risk of uterine leiomyoma. Hum Reprod. 2005; 20(6): 1586-9.
• 5. Chang WS, Tsai CW, Wang JY, et al. Contribution of X-Ray Repair Complementing Defective Repair in Chinese Hamster Cells 3 (XRCC3) Genotype to Leiomyoma Risk. Anticancer Res. 2015; 35(9): 4691-6.
• 6. Hsieh YY, Chang CC, Bau DT, Yeh LS, Tsai FJ, Tsai CH. X-ray repair cross-complementing group 4 (XRCC4) promoter -1394( *)T-related genotype, but not XRCC4 codon 247/intron 3 or xeroderma pigmentosum group D codon 312, 751/promoter -114, polymorphisms are correlated with higher susceptibility to myoma. Fertil Steril. 2008; 90(4): 1417-23.
• 7. Wang H, Mahadevappa M, Yamamoto K, et al. Distinctive proliferative phase differences in gene expression in human myometrium and leiomyomata. Fertil Steril. 2003; 80(2): 266-76.
• 8. Dimitrova IK, Richer JK, Rudolph MC, et al. Gene expression profiling of multiple leiomyomata uteri and matched normal tissue from a single patient. Fertil Steril. 2009; 91(6): 2650-63.
• 9. Smith CW, Anderson DC. PMN adhesion and extravasation as a paradigm for tumor cell dissemination. Cancer Metastasis Rev. 1991;10(1): 61-78.
• 10. Tacyıldız N, Cavdar AO. Adezyon molekülleri ve metastaz. Ankara Tıp Mecmuası. 1995; 48: 199-214.
• 11. McEver RP, Cheng Zhu. Rolling cell adhesion. Annu Rev Cell Dev Biol. 2010; 26: 363-96.
• 12. Fraemohs L, Koenen RR, Ostermann G, Heinemann B, Weber C. The functional interaction of the beta 2 integrin lymphocyte function-associated antigen-1 with junctional adhesion molecule-A is mediated by the I domain. J Immunol. 2004;173(10): 6259-64.
• 13. Williams LA, Martin-Padura I, Dejana E, Hogg N, simmons DL. Identification and characterisation of human Junctional Adhesion Molecule (JAM). Mol Immunol. 1999; 36(17): 1175-88.
• 14. Martìn-Padura I, Lostaglio S, Schneemann M, et al, Junctional adhesion molecule, a novel member of the immunoglobulin superfamily that distributes at intercellular junctions and modulates monocyte transmigration. J Cell Biol. 1998; 142(1): 117-27.
• 15. Novelli G, Borgiani P, Giardina E, et al. Role of genetics in prevention of coronary atherosclerosis. Curr Opin Cardiol. 2003; 18(5): 368-71.
• 16. Tokat B, Ozturk T, Seyhan MF, et al. Interactive Effects of Common Haplotypes of Two Leukocyte Diapedesis-Related Genes, LFA- 1 and JAM-A on Breast Cancer Risk. Int J Hematol Oncol. 2018;(1): 45-52.
• 17. Ong KL, Leung RYH, Wong LYF, et al. Association of F11 receptor gene polymorphisms with central obesity and blood pressure. J Intern Med. 2008; 263(3): 322-32.
• 18. Park SR, Park KS, Park YJ, Bang D, Lee ES. CD11a, CD11c, and CD18 gene polymorphisms and susceptibility to Behçet's disease in Koreans. Tissue Antigens. 2014; 84(4): 398-404.
• 19. Wojcikiewicz EP, Koenen RR, Fraemohs L, et al. LFA-1 binding destabilizes the JAM-A homophilic interaction during leukocyte transmigration. Biophys J. 2009; 96(1): 285-93.
• 20. Bae SM, Kim YW, Lee JM, Namkoong SE, Kim CK, Ahn WS. Expression profiling of the cellular processes in uterine leiomyomas: omic approaches and IGF-2 association with leiomyosarcomas. Cancer Res Treat. 2004; 36(1): 31-42.
• 21. Fu Z, Jiao M, Zhang M, et al. LFA-1 gene polymorphisms are associated with the sporadic infiltrative duct breast carcinoma in Chinese Han women of Heilongjiang Province. Breast Cancer Res Treat. 2011; 127(1): 265-71.
• 22. Tokat B, Kurt O, Bugra Z, Ozturk O, Yilmaz-Aydogan H. Investigation of the monocyte diapedesis-related LFA-1 and JAM-A gene variants in Turkish coronary heart disease patients. Meta Gene. 2014; 2: 1-10.
• 23. Ostermann G, Fraemohs L, Baltus T, et al., Involvement of JAM-A in mononuclear cell recruitment on inflamed or atherosclerotic endothelium: inhibition by soluble JAM-A. Arterioscler Thromb Vasc Biol. 2005; 25(4): 729-35.
• 24. Sladojevic N, Stamatovic SM, Keep RF, et al. Inhibition of junctional adhesion molecule-A/LFA interaction attenuates leukocyte trafficking and inflammation in brain ischemia/reperfusion injury. Neurobiol Dis. 2014; 67: 57-70.
• 25. Pence HH , Caykara B, Pence S, Tokat B, Otunctemur A. Effects of JAM-A rs790056 and LFA-1 rs8058823 Variations on Kidney Cancer. JAREM. 2019; 21:22.
• 26. Çaykara B, Alsaadoni H, Pençe HH, Pençe S, Aydoğan HY, Taştekin D. Investigation of JAM-A (rs790056) and LFA-1 (rs8058823) gene variants in Turkish colorectal cancer patients. Turk J Gastroenterol. 2019;30(10): 872-876.
• 27. Moor AB, Flake GP, Swartz Cd, et al. Association of race, age and body mass index with gross pathology of uterine fibroids. J Reprod Med. 2008; 53(2): 90.
• 28. Shen Y, Xu Q, XU J, Ren ML, Cai YL. Environmental exposure and risk of uterine leiomyoma: an epidemiologic survey. Eur Rev Med Pharmacol Sci. 2013; 17(23): 3249-56.