Daidzein Inhibits Pentylenetetrazol-Induced Seizures in Mice
Abstract
Background: Phytomolecules, through their antioxidant and anti-inflammatory effects, can improve neurodegenerative diseases. The aim of this study was to evaluate the effect of daidzein, a phytoestrogenic agent, on seizure severity and oxidative stress in a pentylenetetrazol (PTZ)-induced seizure model.
Methods: Male mice were injected intraperitoneally (i.p.) with daidzein (1 and 5 mg/kg) and, 30 minutes later, a single convulsive dose of PTZ was administered. The groups were as follows: PTZ Control, PTZ+Diazepam, PTZ+Daidzein 1 mg/kg, PTZ+Daidzein 5 mg/kg, and PTZ+DMSO. Seizure onset and seizure stages were scored over a 30-minute observation period according to the Racine classification. Serum samples were collected to evaluate oxidative stress parameters using an automated colorimetric method.
Results: Administration of daidzein at a dose of 1 mg/kg (first seizure presentation and modified stage p=0.001), but not 5 mg/kg, inhibited seizure formation similarly to diazepam. Neither dose of daidzein (1 and 5 mg/kg) increased serum antioxidant parameters (total thiol (p=0.425), native thiol(p=0.350)).
Conclusion: Low-dose daidzein can prevent seizure while causing a smaller increase in oxidative stress parameters compared to diazepam suggests that it may have potential as an anticonvulsant agent.
Keywords
Supporting Institution
TÜBİTAK 2209
Project Number
1919B012105107
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