Farelerde Pentilentetrazol ile Oluşturulmuş Konvülzyonda Daidzein Nöbet Oluşumunu Engeller

Year 2026, Volume: 7 Issue: 1, 42 - 49, 31.01.2026

Hümeyra Çelik , Canan Akünal Türel , Murat Alışık , Cansu Kara Öztabağ , Şule Nur Türk

https://doi.org/10.47482/acmr.1645687

Abstract

Amaç: Fitomoleküller, antioksidan ve anti-inflamatuar etkileri sayesinde nörodejeneratif hastalıkları iyileştirebilir. Bu çalışmanın amacı, fitoöstrojenik bir ajan olan daidzeinin, pentilentetrazol (PTZ) kaynaklı nöbet modelinde nöbet şiddeti ve oksidatif stres üzerindeki etkisini değerlendirmektir.
Yöntem: Erkek farelere intraperitoneal olarak daidzein (1 ve 5 mg/kg) enjekte edildi ve 30 dakika sonra tek bir konvülsif doz PTZ uygulandı. Gruplar şu şekildeydi: PTZ Kontrol, PTZ+Diazepam, PTZ+Daidzein 1 mg/kg, PTZ+Daidzein 5 mg/kg ve PTZ+DMSO. Nöbet başlangıcı ve nöbet evreleri, Racine sınıflandırmasına göre 30 dakikalık bir gözlem süresi boyunca puanlandı. Serum örnekleri, otomatik kolorimetrik bir yöntem kullanılarak oksidatif stres parametrelerini değerlendirmek için toplandı.
Bulgular:Daidzeinin 1 mg/kg dozunda (ilk nöbet sunumu ve modifiye evre p=0.001) uygulanması, 5 mg/kg uygulanması hariç, diazepam ile benzer şekilde nöbet oluşumunu engelledi. Daidzeinin her iki dozu da (1 ve 5 mg/kg) serum antioksidan parametrelerini (total tiyol (p=0.425), nahiv tiyol (p=0.350)) artırmadı.
Sonuçlar: Diazepam ile karşılaştırıldığında düşük dozda daidzeinin, oksidatif stres parametrelerinde daha az artışa neden olup nöbetleri önleyebilmesi, daidzeinin potansiyel antikonvülsan bir ajan olabileceğini düşündürmektedir.

Keywords

daidzein , epileptic nöbet , oksidatif stress

Project Number

1919B012105107

Daidzein Inhibits Pentylenetetrazol-Induced Seizures in Mice

Abstract

Background: Phytomolecules, through their antioxidant and anti-inflammatory effects, can improve neurodegenerative diseases. The aim of this study was to evaluate the effect of daidzein, a phytoestrogenic agent, on seizure severity and oxidative stress in a pentylenetetrazol (PTZ)-induced seizure model.
Methods: Male mice were injected intraperitoneally (i.p.) with daidzein (1 and 5 mg/kg) and, 30 minutes later, a single convulsive dose of PTZ was administered. The groups were as follows: PTZ Control, PTZ+Diazepam, PTZ+Daidzein 1 mg/kg, PTZ+Daidzein 5 mg/kg, and PTZ+DMSO. Seizure onset and seizure stages were scored over a 30-minute observation period according to the Racine classification. Serum samples were collected to evaluate oxidative stress parameters using an automated colorimetric method.
Results: Administration of daidzein at a dose of 1 mg/kg (first seizure presentation and modified stage p=0.001), but not 5 mg/kg, inhibited seizure formation similarly to diazepam. Neither dose of daidzein (1 and 5 mg/kg) increased serum antioxidant parameters (total thiol (p=0.425), native thiol(p=0.350)).
Conclusion: Low-dose daidzein can prevent seizure while causing a smaller increase in oxidative stress parameters compared to diazepam suggests that it may have potential as an anticonvulsant agent.

Keywords

daidzein , epileptic seizure , oxidative stress

Supporting Institution

TÜBİTAK 2209

Project Number

1919B012105107

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