Daidzein Inhibits Pentylenetetrazol-Induced Seizures in Mice
Öz
Background: Phytomolecules, through their antioxidant and anti-inflammatory effects, can improve neurodegenerative diseases. The aim of this study was to evaluate the effect of daidzein, a phytoestrogenic agent, on seizure severity and oxidative stress in a pentylenetetrazol (PTZ)-induced seizure model.
Methods: Male mice were injected intraperitoneally (i.p.) with daidzein (1 and 5 mg/kg) and, 30 minutes later, a single convulsive dose of PTZ was administered. The groups were as follows: PTZ Control, PTZ+Diazepam, PTZ+Daidzein 1 mg/kg, PTZ+Daidzein 5 mg/kg, and PTZ+DMSO. Seizure onset and seizure stages were scored over a 30-minute observation period according to the Racine classification. Serum samples were collected to evaluate oxidative stress parameters using an automated colorimetric method.
Results: Administration of daidzein at a dose of 1 mg/kg (first seizure presentation and modified stage p=0.001), but not 5 mg/kg, inhibited seizure formation similarly to diazepam. Neither dose of daidzein (1 and 5 mg/kg) increased serum antioxidant parameters (total thiol (p=0.425), native thiol(p=0.350)).
Conclusion: Low-dose daidzein can prevent seizure while causing a smaller increase in oxidative stress parameters compared to diazepam suggests that it may have potential as an anticonvulsant agent.
Anahtar Kelimeler
daidzein, epileptic seizure, oxidative stress
Destekleyen Kurum
TÜBİTAK 2209
Proje Numarası
1919B012105107
Kaynakça
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