Beta vulgaris L. var. cicla verilen sıçanların akciğer, karaciğer ve böbreklerindeki kollajen miktarının biyokimyasal ve histolojik analizi

Year 2025, Volume: 8 Issue: 1, 76 - 83, 28.02.2025

Burcin Alev , Aleyna Muhan , Şehkar Oktay , Esin Ak , Sevim Tunalı , Refiye Yanardağ , Ayşen Yarat

https://doi.org/10.53446/actamednicomedia.1627421

Abstract

Amaç: Kollajen, ekstraselüler matriksin (ECM) temel bir bileşenidir ve organ yapısı, hücresel işlevler ve yara iyileşmesinde kritik bir rol oynar. Beta vulgaris L. var. cicla (pazı), vitaminler, flavonoidler ve nitratlar dahil olmak üzere çeşitli biyoaktif bileşenleriyle bilinir. Pazı, antioksidan, anti-inflamatuar ve antidiyabetik etkiler gibi çok sayıda sağlık yararıyla ilişkilendirilmiştir. Bu çalışma, pazının akciğer, karaciğer ve böbrek gibi hayati organlardaki kollajen miktarı üzerindeki etkisini araştırmaktadır.
Yöntem: Sıçanlar iki gruba ayrıldı: kontrol ve pazı verilen grup. Pazı ekstresi sıçanlara 7 gün boyunca günde 100 mg/kg dozda verildi. 8. günde sıçanlar sakrifiye edildi ve akciğer, böbrek ve karaciğer dokuları toplandı. Kollajen miktarı hem biyokimyasal hem de histolojik analizler kullanılarak ölçüldü.
Bulgular: Pazı uygulaması, kollajen içeriği üzerinde dokuya özgü etkiler gösterdi: akciğerdeki kollajeni artırdı, karaciğerdeki kollajeni önemli ölçüde azalttı ve böbrek kollajeni üzerinde hiçbir etkisi olmadı. Bu biyokimyasal değişiklikler, akciğerdeki ve böbrekteki histolojik sonuçlarla desteklendi; ancak karaciğerde önemli bir histolojik değişiklik gözlenmedi. Bu çeşitli etkiler, dokular arasında kollajen metabolizmasındaki ve düzenleyici mekanizmalardaki farklılıklarla ilişkili olabilir.
Sonuç: Bulgular pazının kollajen sentezi ve ECM’nin yeniden şekillenmesi üzerindeki belirgin etkileri nedeniyle yara iyileşmesi, doku güçlendirme ve antifibrotik tedavi gibi uygulamalar için potansiyel bir terapötik ajan olarak umut vadettiğini göstermektedir. Pazının klinik uygulamalardaki potansiyelini tam olarak anlamak için bu etkilerin altında yatan mekanizmalar üzerine daha fazla çalışma gereklidir.

Keywords

Pazı, kollajen miktarı, akciğer, böbrek, karaciğer

Biochemical and histological analysis of collagen content in lungs, liver and kidneys of rats treated with Beta vulgaris L. var. cicla

Abstract

Objective: Collagen is a fundamental component of the extracellular matrix (ECM) and plays a critical role in organ structure, cellular functions, and wound healing. Beta vulgaris L. var. cicla (chard) is known for its diverse bioactive compounds, including vitamins, flavonoids, and nitrates. Chard has been associated with numerous health benefits, such as antioxidant, anti-inflammatory, and antidiabetic effects. This study investigates the impact of chard on collagen content in vital organs, specifically the lungs, liver, and kidneys.
Methods: The rats divided into two groups: the control and the chard given group. The chard extract was administered to rats at a dose of 100 mg/kg per day for 7 days. On the 8th day, the rats were sacrificed, and tissues from the lungs, kidneys, and liver were collected. The collagen content was measured using both biochemical and histological analyses.
Results: Chard administration exhibited tissue-specific effects on collagen content: it increased collagen in the lungs, decreased it in the liver significantly, and had no effect on kidney collagen. These biochemical changes were supported by histological results in the lungs and kidneys; however, no significant histological changes were observed in the liver. These varied effects might be related to differences in collagen metabolism and regulatory mechanisms across tissues.
Conclusion: The findings suggest that chard, due to its distinct effects on collagen synthesis and ECM remodeling, holds promise as a potential therapeutic agent for applications such as wound healing, tissue strengthening, and antifibrotic therapy. Further studies on the mechanisms underlying these effects are necessary to fully understand the potential of chard in clinical applications.

Keywords

Chard, collagen content, lung, kidney, liver

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