In vitro antibacterial activity of Origanum onites and Mentha spicata subs tomentosa essential oil nanoemulsions against bacterial fish pathogens

Year 2022, , 495 - 504, 01.12.2022

Mevlüt Nazıroğlu , Öznur Diler , Öznur Özil , Abdullah Diler

https://doi.org/10.22392/actaquatr.1145109

Cited By: 1

Abstract

The current study aimed to determine the antimicrobial activities of two different aromatic plants (Origanum onites, Mentha spicata subs. tomentosa) essential oils, and their nanoemulsion formulations against six common fish pathogens, Pseudomonas aeruginosa, Vibrio parahaemolyticus, Aeromonas veronii, Vibrio alginolyticus, Yersinia ruckeri, and Lactococcus garvieae. The main components of Mentha spicata subs. tomentosa essential oil (MEO) were piperitone (25.01%), eucalyptol (1,8-cineole) (19.53%), pulegone (14.50%) and, Piperitenone (10.98%). The major components of Origanum onites essential oil (OEO) which were carvacrol (46.17%) and, p-cymene (13.05%) were detected. The antibacterial effects of OEO and MEO and their nanoemulsions were determined by using the agar disc diffusion method. The OEO and its nanoemulsions were extremely effective against the Gram‐negative Aeromonas veronii than the positive control (enrofloxacin). In addition, it was observed that OEO nanoemulsion was more effective than OEO in terms of antibacterial activity. MEO and its nanoemulsions were found to have low activity against fish pathogens, however, there was no activity on Aeromonas veronii and Lactococcus garvieae.

Keywords

Origanum onites, Mentha spicata subs. Tomentosa, Bacterial fish pathogen, Antibacterial activity

Origanum onites ve Mentha spicata subs. tomentosa Uçucu Yağ Nanoemülsiyonlarının Bakteriyel Balık Patojenlerine Karşı in Vitro Antibakteriyel Aktivitesi

Abstract

Bu çalışmanın amacı, iki farklı aromatik bitkinin (Origanum onites, Mentha spicata subs. tomentosa) uçucu yağlarının ve bunların nanoemülsiyon formülasyonlarının altı farklı balık patojeni Pseudomonas aeruginosa, Vibrio parahaemolyticus, Aeoromonas veronii, Yersinia ruckeri, Lactococcus garvieae ve Vibrio alginolyticus'a karşı antimikrobiyal aktivitelerini belirlemektir. Mentha spicata subs. tomentosa uçucu yağının (MEO) ana bileşenleri piperitone, okaliptol (1,8-cineole), pulegone, piperitenone sırasıyla %25.01, %19.53, %14.50 ve %10.98 olarak belirlenmiştir. Origanum onites uçucu yağının (OEO) ana bileşenleri sırasıyla karvakrol, %46.17 ile p-cymene, %13.05 oranında tespit edilmiştir. OEO ve MEO ve bu uçucu yağların nanoemülsiyonlarının antibakteriyel etkileri agar disk difüzyon yöntemi kullanılarak belirlendi. OEO nanoemülsiyonları, Gram-negatif Aeromonas veronii'ye karşı pozitif kontrole (enrofloksasin) göre son derece etkili olduğu tespit edilmiştir. Bunun yanında, antibakteriyel aktivite bakımından OEO nanoemülsiyonlarının, OEO’ya göre daha etkili olduğu görülmüştür. MEO ve nanoemülsiyonlarının balık patojenlerine karşı düşük bir aktiviteye sahipken Aeromonas veronii ve Lactococcus garvieae üzerinde antibakteriyel aktivite göstermemiştir.

Keywords

Origanum onites, Mentha spicata subs. Tomentosa, Bakteriyel balık patojenleri, Antibakteriyel aktivite

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