First Report of Candida sp. from Rainbow Trout (Oncorhynchus mykiss, Walbaum) Fry in Turkey

Yıl 2019, , 55 - 59, 01.03.2019

Seçil Metin , Behire İşıl Didinen , Ayşegül Kubilay , Bülent Baş , Pınar Yıldırım , Tom Wıklund

https://doi.org/10.22392/egirdir.437661

Cited By: 1

Öz

In this study, we isolated Candida
sp. for the first time from fry of rainbow trout (Oncorhynchus mykiss) in Turkey. Candida species caused disease in fry (average 0.2 g) which
was observed at 10°C water temperature in February 2016. Due to yeast
accumulation on gills, whitish areas was seen and fusion of secondary lamella
in gills were observed under microscope. The microbiological samples of kidney,
spleen and gills were streaked onto trypticase soy agar (TSA) and tryptone
yeast extract salts (TYES) agar plates
and incubated at 22°C in TSA and 18°C in TYES agar for 48 h. Thirteen isolates
were obtained and Gram staining was performed. The all of the isolates were determined as yeast cell
based on cellular morphology by using light microscopy. The identification was performed by ITS (Internal transcribed spacer) region
sequence analysis and sequence data demonstrated that representative isolate
D11 belonged to genus Candida. The
yeast infection might have seen in early development stages (larvae and fry)
due to the intensive antibiotic use against rainbow trout fry syndrome (RTFS)
disease.

Anahtar Kelimeler

Candida sp., rainbow trout, Oncorhynchus mykiss, identification, sequence analysis

Kaynakça

• Achkar, J. M., & Fries, B. C. (2010). Candida infections of the genitourinary tract. Clinical Microbiology Reviews, 23(2), 253-273.
• Chi, Z. M., Liu, G., Zhao, S., Li, J., & Peng, Y. (2010). Marine yeasts as biocontrol agents and producers of bio-products. Applied Microbiology and Biotechnology, 86(5), 1227-1241.
• Gago, S., Alastruey-Izquierdo, A., Marconi, M., Buitrago, M. J., Kerhornou, A., Kersey, P. J., Mellado, E., Cuenca-Estrella, M., Rodríguez-Tudela, J.L., & Cuesta, I. (2014).
• Ribosomic DNA intergenic spacer 1 region is useful when identifying Candida parapsilosis spp. complex based on high-resolution melting analysis. Medical Mycology, 52(5), 472-481.
• Galuppi, R., Fioravanti, M., Delgado, M., Quaglio, F., Caffara, M., & Tampieri, M. (2001). Segnalazione di due casi do micosi della vescica natatoria in Sparus aurata e Carrassius auratus. Boll Soc Ital Patol., 32, 26-34.
• Gatesoupe, F. J. (2007). Live yeasts in the gut: natural occurrence, dietary introduction, and their effects on fish health and development. Aquaculture, 267(1-4), 20-30.
• Gatesoupe, F. J., Aubin, J., Quentel, C., & Labbé, L. (2005). Ofimer probiotic study on rainbow trout. IV. The settlement of intestinal microbiota in rainbow trout (Oncorhynchus mykiss) fry submitted to probiotic treatment. Larvi, 4, 180-183.
• Hatai, K., & Egusa, S. (1975). Candida sake from gastro-tympanites of Amago, Oncorhynchus rhodurus. Bulletin of the Japanese Society of Scientific Fisheries, 41, 993.
• Holt, J. G., Krieg, N. R., Sneath, P. A., Staley, J. T., & Williams, S. T. (1994). Bergey's Manual of Determinate Bacteriology. 9th edn. Williams & Wilkins, Baltimore.
• Kutty, S. N., & Philip, R. (2008). Marine yeasts—a review. Yeast, 25(7), 465-483.
• Meurman, J. H., Siikala, E., Richardson, M., & Rautemaa, R. (2007). Non-Candida albicans Candida yeasts of the oral cavity. Communicating Current Research and Educational Topics and Trends in Applied Microbiology, 1(1), 719-731.
• Moore, M. M., & Strom, M. S. (2003). Infection and mortality by the yeast Metschnikowia bicuspidata var. bicuspidata in chinook salmon fed live adult brine shrimp (Artemia franciscana). Aquaculture, 220 (1-4), 43-57.
• Mueller, G. J., & Whisler, H. C. (1994). Fungal parasites of salmon from the Columbia River watershed. Salmon Saprolegniasis. Bonneville Power Administration, Div. Fish and Wildlife, Portland, OR, 163-187.
• Park, S. W., Yu, J. H., & Han, J. J. (2012). Studies on the overinflation of the Cardiac Stomach in Sterlet Sturgeon Acipenser ruthenus, fingerlings. Journal of Fish Pathology, 25(1), 59-65.
• Ross, S. S., & Morris, E. O. (1965). An investigation of the yeast flora of marine fish from Scottish coastal waters and a fishing ground off Iceland. Journal of Applied Microbiology, 28(2), 224–234.
• Sakata, T., Oshiro, T., & Teshima, S.I. (1993). Characteristics and fatty acid composition of yeast isolated from the intestines of rainbow trout Oncorhynchus mykiss. Memoirs of Faculty of Fisheries, Kagoshima University, 42, 51–59.