Spirulina platensis’ten mavi pigment fikosiyanin ekstraksiyonu

Abstract

Fikosiyanin, Spirulina platensis’ten elde edilen ve suda çözünebilen mavi pigmenttir. Bu çalışmada fikosiyanin ekstraksiyonu için çözücü olarak su kullanılmıştır. Fikosiyanin konsantrasyonunu (PC) (mg mL-1) belirlemek için yaş Spirulina biyoması, oda sıcaklığında 48 saat beklemiş yaş biyomas, 48 saat dondurulmuş Spirulina, 15 gün dondurulmuş Spirulina ve kuru Spirulina kullanılmıştır. Aynı zamanda Fikosiyanin saflığı (OD615/OD280) ve ürün miktarı (mg g-1) saptanmıştır. Onbeşgün dondurulmuş Spirulina,48 saat oda sıcaklığında beklemiş yaş biyomas ve yaş Spirulina biyomasından, sırasıyla 17,6497, 17,1370 ve 17,0833 mg g-1fikosiyanin elde edilmiştir. Üründeki saflık oranları (OD615/OD280), 4.60, 0.81, 0.85, 0.98 ve 6.55, sırasıyla onbeşgün dondurulmuş Spirulina, 48 saat dondurulmuş Spirulina, 48 saat bekletilmiş yaş Spirulina biyoması, kurutulmuş Spirulina ve yaş biyomastan elde edilmiştir.

Keywords

• Fikosiyanin
• ekstraksiyon
• çözücü
• C-PC ürün

Blue colored pigment phycocyanin extraction from Spirulina platensis

Abstract

Phycocyanin is a blue pigment and water-soluble biliprotein from the Spirulina platensis. In this study, the water was used as a solvent for the extraction of phycocyanin. Wet biomass of Spirulina, wet biomass held for 48 hours at room temperature, frozen Spirulina for 48 hours, and frozen biomass for fifteen days, and dried Spirulina was used to determine phycocyanin concentration (PC) (mg mL-1). At the same time, extract purity of phycocyanin (OD615/OD280) and yield (mg g-1) were determined. Phycocyanin yield of 17,6497, 17,1370, and 17,0833 mg g-1 were obtained from frozen Spirulina for fifteen days, wet biomass of Spirulina held for 48 hours, and wet biomass of Spirulina, respectively. The purity ratios (OD615/OD280) of 4.60, 0.81, 0.85, 0.98, and 6.55 were determined for frozen for 15 days, frozen Spirulina for 48 hours, wet biomass of Spirulina (waited for 48 hours), dried Spirulina and wet Spirulina, respectively

Keywords

• Phycocyanin
• extraction
• solvent
• C-PC yield

References

1. Referans1 Abalde, J., Betancourt, L., Torres, E., Cid, A., & Barwell, C. (1998). Purification and characterization of phycocyanin from the marine cyanobacterium Synechococcus sp. 109201. Plant Science, 136(1), 109-120.
2. Referans2 Bennett, A., & Bogorad, L. (1973). Complomentary chromatic adaptation in a filamentous blue-green alga. J. Cell Biol. 58, 419-435.
3. Referans3 Bermejo, R., Acien, F. G., Ibanez, M. J., Fernandez, J. M., Molina, E., & Alvarez-Pez, J. M. (2003). Preparative purification of B-phycoerythrin from the microalga Porphyridium cruentum by expanded-bed adsorption chromatography. J. Chromatogr. B 790, 317-325.
4. Referans4 Eriksen, N. T. (2008). Production of phycocyanin-a pigment with applications in biology, biotechnology, foods and medicine. Appl. Microbiol. Biotechnol. 80, 1-14.
5. Referans5 Herrera, A., Boussiba, S., Napoleone, V., & Hohlberg, A. (1989). Recovery of c-phycocyanin extract. Bioresour. Technol. 100, 5312-5317.
6. Referans6 Ilter, I., Akyıl, S., Demirel, C., Koç, M., Dalay, M. C. & Ertekina, F.K. (2018). Optimization of phycocyanin extraction from Spirulina platensis using different techniques. Journal of Food Composition and Analysis, 70, 78-88.
7. Referans7 Jaquen, P., Lepine, B., Rossignol, N., Royer, R. & Quem’ eneur, F. (1999). Clarification and concentration with membrane technology of a phycocyanin solution extracted from Spirulina platensis. Biotechnology Techniques 13: 877–881.
8. Referans8 Liu, L.N., Chen, X.L., Zhang, X.Y., Zhang, Y. Z., & Zhou, B. C. (2005). One-step chromatography method for efficient seperation and purification of R-phycoerythrin from Polysiphonia urceolata. J. Biotechnology 116, 91-100.

9. Referans9 Moraes, C., Kalıl, S. J. & Burke, J. F. M. (2010). C-Phycocyanin extraction process for large-scale use. J. of Food Biochemistry, 34(s1) 133-148.
10. Referans10 Nakagawa, K., Ritcharoen, W., Sri-Uam, P., Prasert, P. & Shuji, A. (2016). Antioxidant properties of convective-air-dried Spirulina maxima: Evaluation of phycocyanin retention by a simple mathematical model of air-drying. Food and Bioproducts Processing 100, 292-302.
11. Referans11 Reis, A., Mendes, A., Lobo-Fernandes, H., Empis, J. A., & Novais, J. M. (1998). Production, extraction and purification of phycobiliproteins from Nostoc sp. Bioresour. Technol. 66, 181-187.
12. Referans12 Romay Ch., Gonzales, R., Ledon, N., Remirez, D., & Rimbau, V. (2003). C-phycocyanin: a biliprotein with antioxidant, anti-inflammatory and neuroprotective effects. Curr. Protein Pept. Sci. 4, 207-216.
13. Referans13 Sarada, R., Pillai, M. G. & Ravishankar; G. A. (1999). Phycocyanin from Spirulina sp: influence of processing of biomass on phycocyanin yield, analysis of efficacy of extraction methods and stability studies on phycocyanin. Process Biochemistry 34, 795-801.
14. Referans14 Silveira, S.T., Burkert, J. F. M., Costa, J. A. V., Burkert, S. J., & Kalil, S. J. (2007). Optimization of phycocyanin extraction from Spirulina platensis using factorial design. Bioresource Technology 98 1629-1634.
15. Referans15 Vonshak, A. (1997). Spirulina Platensis Arthrospira: Physiology, Cell-Biology And Biotechnology (A. Vonshak, ed.) 233 p. Taylor&Francis, London.