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The Effect of Ovarian Fluid on Motility Characteristics and Fertilization Ratio of Rainbow Trout (Onchorhynchus mykiss) Sperm

Year 2020, Volume 16, Issue 2, 266 - 274, 01.06.2020
https://doi.org/10.22392/actaquatr.660155

Abstract

In this study, the effect of ovarian fluid and hatchery water as activation solutions for sperm activation in rainbow trout (Oncorhynchus mykiss) on motility (%) curvilinear velocity (VCl, µm / sec) and fertilization rate were analyzed. Three female and six male fish belonging to the 3+ age group were used in the study. Each females' egg was fertilized with analyzed sperm samples both either removing ovarian fluid or not. Between results of the motility (%) values of sperm samples that activated with hatchery water and ovarian fluid were found statistically different (p <0.05); the difference between the kinematic parameters of motility was insignificant (p˃0.05) among group. There was no difference between the fertilization rate in the groups. 

References

  • Alonzo, S.H., Stiver, K. A.,&Marsh-Rollo, S.E. (2016). Ovarian fluid allows directional cryptic female choice despite external fertilization. Nature Communications, 7, 12452.
  • Barannikova, I. A., Dyubin, V. P., Bayunova, L. V., & Semenkova, T. B. (2002). Steroids in the control of reproductive function in fish. Neuroscience and Behavioral physiology, 32(2), 141-148.
  • Bayunova, L. V., Barannikova, I. A., Dyubin, V. P., Gruslova, A. B., Semenkova, T. B., & Trenkler, I. V. (2003). Sex steroids concentrations in Russian sturgeon (Acipenser guel denstaedti Br.) serum and coelomic fluid at final oocyte maturation. Fish Physiology and Biochemistry, 28(1-4), 325-326.
  • Billard, R., Cosson, M. (1992). Some problems related to the assessment ofsperm motility in freshwater fish. Journal of Experimental Biology. 261, 122-131.
  • Butts, I. A., Johnson, K., Wilson, C. C., & Pitcher, T. E. (2012). Ovarian fluid enhances sperm velocity based on relatedness in lake trout, Salvelinus namaycush. Theriogenology, 78(9), 2105-2109.
  • Butts, I. A., Prokopchuk, G., Kašpar, V., Cosson, J., & Pitcher, T. E. (2017). Ovarian fluid impacts flagellar beating and biomechanical metrics of sperm between alternative reproductive tactics. Journal of Experimental Biology, 220 (12), 2210-2217.
  • Cosson, J. (2004). The ionic and osmotic factors controlling motility of fish spermatozoa. Aquaculture International, 12(1), 69-85.
  • Cosson, J. (2008) Methods to analyse the movements of the spermatozoa and their flagella. In: Alavi SMH, Cosson J, Coward K, Rafiee G (eds) Fish spermatology. Alpha Sci- ence Ltd, Oxford, pp 63–102.
  • Cosson, J. (2010) Frenetic activation of fish spermatozoa flagella entails short-term motility, portending their precocious decadence. Journal of Fish Biology 76:240–279.
  • Dietrich, G. J., Wojtczak, M., Słowińska, M., Dobosz, S., Kuźmiński, H., & Ciereszko, A. (2008). Effects of ovarian fluid on motility characteristics of rainbow trout (Oncorhynchus mykiss Walbaum) spermatozoa. Journal of Applied Ichthyology, 24(4), 503-507.
  • Diogo, P., Soares, F., Dinis, M. T., & Cabrita, E. (2010). The influence of ovarian fluid on Solea senegalensis sperm motility. Journal of Applied Ichthyology, 26(5), 690-695.
  • Elofsson, H., Van Look, K., Borg, B., & Mayer, I. (2003). Influence of salinity and ovarian fluid on sperm motility in the fifteen‐spined stickleback. Journal of Fish Biology, 63(6), 1429-1438.
  • Galvano, P. M., Johnson, K., Wilson, C. C., Pitcher, T. E., & Butts, I. A. (2013). Ovarian fluid influences sperm performance in lake trout, Salvelinus namaycush. Reproductive biology, 13(2), 172-175.
  • Golpour, A., Akhoundian, M., Khara, H., Rahbar, M., & Dadras, H. (2013). Changes of sperm quality parameters in Caspian roach (Rutilus rutilus caspicus) during spawning migration. Czech Journal of Animal Science, 3, 117-124.
  • Hatef, A., Niksirat, H., & Alavi, S. M. H. (2009). Composition of ovarian fluid in endangered Caspian brown trout, Salmo trutta caspius, and its effects on spermatozoa motility and fertilizing ability compared to freshwater and a saline medium. Fish physiology and biochemistry, 35(4), 695-700.
  • Hirano, T., Morisawa, M., Suzuki, K. (1978). Changes in plasma and coelomic fluid composition of the mature salmon (Onchorhynchus keta) during freshwater addition. Comparative Biochemistry and Physiology, A 61, 5–8.
  • İnanan, B. E., & Öğretmen, F. (2015). Determination of differences in the biochemical properties of sperm activating and non-activating ovarian fluids and their influences on sperm motility in rainbow trout (Oncorhynchus mykiss). Aquaculture, 448, 539-544.
  • Jia, Y. D., Niu, H. X., Meng, Z., Liu, X. F., & Lei, J. L. (2015). Biochemical composition of the ovarian fluid and its effects on the fertilization capacity of turbot Scophthalmus maximus during the spawning season. Journal of fish biology, 86(5), 1612-1620.
  • Johnson, S. L., Villarroel, M., Rosengrave, P., Carne, A., Kleffmann, T., Lokman, P. M., & Gemmell, N. J. (2014). Proteomic analysis of chinook salmon (Oncorhynchus tshawytscha) ovarian fluid. PloS one, 9(8), e104155.
  • Kholodnyy, V., Gadêlha, H., Cosson, J., & Boryshpolets, S. (2019). How do freshwater fish sperm find the egg? The physicochemical factors guiding the gamete encounters of externally fertilizing freshwater fish. Reviews in Aquaculture, 1-28.
  • Kime, D. E., Van Look, K. J. W., McAllister, B. G., Huyskens, G., Rurangwa, E., & Ollevier, F. (2001). Computer-assisted sperm analysis (CASA) as a tool for monitoring sperm quality in fish. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 130(4), 425-433.
  • Koya, Y., Munehara, H., Takano, K., & Takahashi, H. (1993). Effects of extracellular environments on the motility of spermatozoa in several marine sculpins with internal gametic association. Comparative Biochemistry and Physiology Part A: Physiology, 106(1), 25-29.
  • Lahnsteiner F., Weisman T. R., Patzner A. (1995). Composition of the ovarian fluid in 4 salmonid species: Onchorhynchus mykiss, Salmo trutta f lacustris, Salvelinus lacustris, and Hucho hucho. Reproduction Nutrition Development 35:465-474. Lahnsteiner, F., Berger, B., Weismann, T., & Patzner, R. A. (1997). Sperm structure and motility of the freshwater teleost Cottus gobio. Journal of Fish Biology, 50(3), 564-574.
  • Lahnsteiner, F. (2002). The influence of ovarian fluid on the gamete physiology in the Salmonidae. Fish Physiology and Biochemistry, 27(1-2), 49-59.
  • Lehnert, S. J., Butts, I. A., Flannery, E. W., Peters, K. M., Heath, D. D., & Pitcher, T. E. (2017). Effects of ovarian fluid and genetic differences on sperm performance and fertilization success of alternative reproductive tactics in Chinook salmon. Journal of evolutionary biology, 30(6), 1236-1245.
  • Lewis, J. A., & Pitcher, T. E. (2017). Tactic‐specific benefits of polyandry in Chinook salmon Oncorhynchus tshawytscha. Journal of fish biology, 90(4), 1244-1256.
  • Litvak, M. K., & Trippel, E.A. (1998). Sperm motility patterns of Atlantic cod (Gadus morhua) in relation to salinity: effects of ovarian fluid and egg presence. Canadian Journal of Fisheries and Aquatic Sciences, 55(8), 1871-1877.
  • Macfarlane, C.P. (2002). Gametic interactions in externally fertilising fishes. PhD Thesis, University of Liverpool. Rakitin, A., Ferguson, M. M., & Trippel, E. A. (1999a). Spermatocrit and spermatozoa density in Atlantic cod (Gadus morhua): correlation and variation during the spawning season. Aquaculture, 170(3-4), 349-358.
  • Rakitin, A., Ferguson, M. M., & Trippel, E. A. (1999b). Sperm competition and fertilization success in Atlantic cod (Gadus morhua): effect of sire size and condition factor on gamete quality. Canadian Journal of Fisheries and Aquatic Sciences, 56(12), 2315-2323.
  • Rosengrave, P., Taylor, H., Montgomerie, R., Metcalf, V., McBride, K., & Gemmell, N. J. (2009). Chemical composition of seminal and ovarian fluids of chinook salmon (Oncorhynchus tshawytscha) and their effects on sperm motility traits. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 152(1), 123-129.
  • Rosengrave, P., Montgomerie, R., & Gemmell, N. (2016). Cryptic female choice enhances fertilization success and embryo survival in chinook salmon. Proceedings of the Royal Society B: Biological Sciences, 283(1827), 20160001.
  • Stoddard, J.W., Parsons, J.E., & Nagler, J.J. (2006). Early onset of embryonic mortality in sub-fertile families of rainbow trout (Oncorhynchus mykiss). Reproduction, Fertility and Development, 17(8), 785-790.
  • Stoss, J. (1983). Fish gamete preservation and spermatozoan physiology. Fish physiology, 9B, 305–350. Turner, E., & Montgomerie, R. (2002). Ovarian fluid enhances sperm movement in Arctic charr. Journal of Fish Biology, 60(6), 1570-1579.
  • Urbach, D., Folstad, I., & Rudolfsen, G. (2005). Effects of ovarian fluid on sperm velocity in Arctic charr (Salvelinus alpinus). Behavioral Ecology and Sociobiology, 57(5), 438-444.
  • Wojtczak, M., Dietrich, G. J., Słowińska, M., Dobosz, S., Kuźmiński, H., & Ciereszko, A. (2007). Ovarian fluid pH enhances motility parameters of rainbow trout (Oncorhynchus mykiss) spermatozoa. Aquaculture, 270(1-4), 259-264.
  • Zadmajid, V., Myers, J.N., Sørensen, S.R., Butts, I.A. (2019). Ovarian fluid and its impacts on spermatozoa performance in fish: a review. Theriogenology. 132:144–152.

Gökkuşağı Alabalığında (Oncorhynchus mykiss) Ovaryum Sıvısının Sperm Motilite Parametreleri ve Döllenme Üzerindeki Etkisi

Year 2020, Volume 16, Issue 2, 266 - 274, 01.06.2020
https://doi.org/10.22392/actaquatr.660155

Abstract

Bu çalışmada, Gökkuşağı alabalığında (Oncorhynchus mykiss) sperm aktivasyonunda aktivasyon solüsyonu olarak kuluçkahane suyuna ilaveten ovaryum sıvısının kullanılmasının motilite (%) ve eğrisel hız (VCl, µm/saniye) üzerindeki etkisi ve ovaryum sıvısı uzaklaştırılmış yumurta örneklerinde döllenme yüzdesi araştırılmıştır. Çalışmada üreme döneminde 3+ yaş grubuna ait üç adet dişi ve altı adet erkek balık kullanılmıştır. Her bir dişe bireye ait yumurta örneklerinde, analizi yapılan sperm örnekleri ile hem ovaryum sıvısı muhafaza edilerek hem de uzaklaştırılarak dölleme gerçekleştirilmiştir. Sperm örneklerine ait motilite (%) değerlerinde kuluçka suyu kullanılarak aktive edilmiş örnekler ile ovaryum sıvısı kullanılarak elde edilmiş örnekler arasındaki sonuçlar istatiksel olarak farklı bulunmuşken (p<0.05); motiliteye ait kinematik parametreler arasındaki fark önemsiz bulunmuştur (p˃0.05). Dölleme grupları arasında ise döllenme yüzdesinde herhangi bir fark bulunmamıştır.

References

  • Alonzo, S.H., Stiver, K. A.,&Marsh-Rollo, S.E. (2016). Ovarian fluid allows directional cryptic female choice despite external fertilization. Nature Communications, 7, 12452.
  • Barannikova, I. A., Dyubin, V. P., Bayunova, L. V., & Semenkova, T. B. (2002). Steroids in the control of reproductive function in fish. Neuroscience and Behavioral physiology, 32(2), 141-148.
  • Bayunova, L. V., Barannikova, I. A., Dyubin, V. P., Gruslova, A. B., Semenkova, T. B., & Trenkler, I. V. (2003). Sex steroids concentrations in Russian sturgeon (Acipenser guel denstaedti Br.) serum and coelomic fluid at final oocyte maturation. Fish Physiology and Biochemistry, 28(1-4), 325-326.
  • Billard, R., Cosson, M. (1992). Some problems related to the assessment ofsperm motility in freshwater fish. Journal of Experimental Biology. 261, 122-131.
  • Butts, I. A., Johnson, K., Wilson, C. C., & Pitcher, T. E. (2012). Ovarian fluid enhances sperm velocity based on relatedness in lake trout, Salvelinus namaycush. Theriogenology, 78(9), 2105-2109.
  • Butts, I. A., Prokopchuk, G., Kašpar, V., Cosson, J., & Pitcher, T. E. (2017). Ovarian fluid impacts flagellar beating and biomechanical metrics of sperm between alternative reproductive tactics. Journal of Experimental Biology, 220 (12), 2210-2217.
  • Cosson, J. (2004). The ionic and osmotic factors controlling motility of fish spermatozoa. Aquaculture International, 12(1), 69-85.
  • Cosson, J. (2008) Methods to analyse the movements of the spermatozoa and their flagella. In: Alavi SMH, Cosson J, Coward K, Rafiee G (eds) Fish spermatology. Alpha Sci- ence Ltd, Oxford, pp 63–102.
  • Cosson, J. (2010) Frenetic activation of fish spermatozoa flagella entails short-term motility, portending their precocious decadence. Journal of Fish Biology 76:240–279.
  • Dietrich, G. J., Wojtczak, M., Słowińska, M., Dobosz, S., Kuźmiński, H., & Ciereszko, A. (2008). Effects of ovarian fluid on motility characteristics of rainbow trout (Oncorhynchus mykiss Walbaum) spermatozoa. Journal of Applied Ichthyology, 24(4), 503-507.
  • Diogo, P., Soares, F., Dinis, M. T., & Cabrita, E. (2010). The influence of ovarian fluid on Solea senegalensis sperm motility. Journal of Applied Ichthyology, 26(5), 690-695.
  • Elofsson, H., Van Look, K., Borg, B., & Mayer, I. (2003). Influence of salinity and ovarian fluid on sperm motility in the fifteen‐spined stickleback. Journal of Fish Biology, 63(6), 1429-1438.
  • Galvano, P. M., Johnson, K., Wilson, C. C., Pitcher, T. E., & Butts, I. A. (2013). Ovarian fluid influences sperm performance in lake trout, Salvelinus namaycush. Reproductive biology, 13(2), 172-175.
  • Golpour, A., Akhoundian, M., Khara, H., Rahbar, M., & Dadras, H. (2013). Changes of sperm quality parameters in Caspian roach (Rutilus rutilus caspicus) during spawning migration. Czech Journal of Animal Science, 3, 117-124.
  • Hatef, A., Niksirat, H., & Alavi, S. M. H. (2009). Composition of ovarian fluid in endangered Caspian brown trout, Salmo trutta caspius, and its effects on spermatozoa motility and fertilizing ability compared to freshwater and a saline medium. Fish physiology and biochemistry, 35(4), 695-700.
  • Hirano, T., Morisawa, M., Suzuki, K. (1978). Changes in plasma and coelomic fluid composition of the mature salmon (Onchorhynchus keta) during freshwater addition. Comparative Biochemistry and Physiology, A 61, 5–8.
  • İnanan, B. E., & Öğretmen, F. (2015). Determination of differences in the biochemical properties of sperm activating and non-activating ovarian fluids and their influences on sperm motility in rainbow trout (Oncorhynchus mykiss). Aquaculture, 448, 539-544.
  • Jia, Y. D., Niu, H. X., Meng, Z., Liu, X. F., & Lei, J. L. (2015). Biochemical composition of the ovarian fluid and its effects on the fertilization capacity of turbot Scophthalmus maximus during the spawning season. Journal of fish biology, 86(5), 1612-1620.
  • Johnson, S. L., Villarroel, M., Rosengrave, P., Carne, A., Kleffmann, T., Lokman, P. M., & Gemmell, N. J. (2014). Proteomic analysis of chinook salmon (Oncorhynchus tshawytscha) ovarian fluid. PloS one, 9(8), e104155.
  • Kholodnyy, V., Gadêlha, H., Cosson, J., & Boryshpolets, S. (2019). How do freshwater fish sperm find the egg? The physicochemical factors guiding the gamete encounters of externally fertilizing freshwater fish. Reviews in Aquaculture, 1-28.
  • Kime, D. E., Van Look, K. J. W., McAllister, B. G., Huyskens, G., Rurangwa, E., & Ollevier, F. (2001). Computer-assisted sperm analysis (CASA) as a tool for monitoring sperm quality in fish. Comparative Biochemistry and Physiology Part C: Toxicology & Pharmacology, 130(4), 425-433.
  • Koya, Y., Munehara, H., Takano, K., & Takahashi, H. (1993). Effects of extracellular environments on the motility of spermatozoa in several marine sculpins with internal gametic association. Comparative Biochemistry and Physiology Part A: Physiology, 106(1), 25-29.
  • Lahnsteiner F., Weisman T. R., Patzner A. (1995). Composition of the ovarian fluid in 4 salmonid species: Onchorhynchus mykiss, Salmo trutta f lacustris, Salvelinus lacustris, and Hucho hucho. Reproduction Nutrition Development 35:465-474. Lahnsteiner, F., Berger, B., Weismann, T., & Patzner, R. A. (1997). Sperm structure and motility of the freshwater teleost Cottus gobio. Journal of Fish Biology, 50(3), 564-574.
  • Lahnsteiner, F. (2002). The influence of ovarian fluid on the gamete physiology in the Salmonidae. Fish Physiology and Biochemistry, 27(1-2), 49-59.
  • Lehnert, S. J., Butts, I. A., Flannery, E. W., Peters, K. M., Heath, D. D., & Pitcher, T. E. (2017). Effects of ovarian fluid and genetic differences on sperm performance and fertilization success of alternative reproductive tactics in Chinook salmon. Journal of evolutionary biology, 30(6), 1236-1245.
  • Lewis, J. A., & Pitcher, T. E. (2017). Tactic‐specific benefits of polyandry in Chinook salmon Oncorhynchus tshawytscha. Journal of fish biology, 90(4), 1244-1256.
  • Litvak, M. K., & Trippel, E.A. (1998). Sperm motility patterns of Atlantic cod (Gadus morhua) in relation to salinity: effects of ovarian fluid and egg presence. Canadian Journal of Fisheries and Aquatic Sciences, 55(8), 1871-1877.
  • Macfarlane, C.P. (2002). Gametic interactions in externally fertilising fishes. PhD Thesis, University of Liverpool. Rakitin, A., Ferguson, M. M., & Trippel, E. A. (1999a). Spermatocrit and spermatozoa density in Atlantic cod (Gadus morhua): correlation and variation during the spawning season. Aquaculture, 170(3-4), 349-358.
  • Rakitin, A., Ferguson, M. M., & Trippel, E. A. (1999b). Sperm competition and fertilization success in Atlantic cod (Gadus morhua): effect of sire size and condition factor on gamete quality. Canadian Journal of Fisheries and Aquatic Sciences, 56(12), 2315-2323.
  • Rosengrave, P., Taylor, H., Montgomerie, R., Metcalf, V., McBride, K., & Gemmell, N. J. (2009). Chemical composition of seminal and ovarian fluids of chinook salmon (Oncorhynchus tshawytscha) and their effects on sperm motility traits. Comparative Biochemistry and Physiology Part A: Molecular & Integrative Physiology, 152(1), 123-129.
  • Rosengrave, P., Montgomerie, R., & Gemmell, N. (2016). Cryptic female choice enhances fertilization success and embryo survival in chinook salmon. Proceedings of the Royal Society B: Biological Sciences, 283(1827), 20160001.
  • Stoddard, J.W., Parsons, J.E., & Nagler, J.J. (2006). Early onset of embryonic mortality in sub-fertile families of rainbow trout (Oncorhynchus mykiss). Reproduction, Fertility and Development, 17(8), 785-790.
  • Stoss, J. (1983). Fish gamete preservation and spermatozoan physiology. Fish physiology, 9B, 305–350. Turner, E., & Montgomerie, R. (2002). Ovarian fluid enhances sperm movement in Arctic charr. Journal of Fish Biology, 60(6), 1570-1579.
  • Urbach, D., Folstad, I., & Rudolfsen, G. (2005). Effects of ovarian fluid on sperm velocity in Arctic charr (Salvelinus alpinus). Behavioral Ecology and Sociobiology, 57(5), 438-444.
  • Wojtczak, M., Dietrich, G. J., Słowińska, M., Dobosz, S., Kuźmiński, H., & Ciereszko, A. (2007). Ovarian fluid pH enhances motility parameters of rainbow trout (Oncorhynchus mykiss) spermatozoa. Aquaculture, 270(1-4), 259-264.
  • Zadmajid, V., Myers, J.N., Sørensen, S.R., Butts, I.A. (2019). Ovarian fluid and its impacts on spermatozoa performance in fish: a review. Theriogenology. 132:144–152.

Details

Primary Language Turkish
Subjects Biology
Journal Section Research Articles
Authors

Güneş YAMANER (Primary Author)
İstanbul Üniveristesi Su Bilimleri Fakültesi
0000-0003-1886-4985
Türkiye

Publication Date June 1, 2020
Published in Issue Year 2020, Volume 16, Issue 2

Cite

APA Yamaner, G. (2020). Gökkuşağı Alabalığında (Oncorhynchus mykiss) Ovaryum Sıvısının Sperm Motilite Parametreleri ve Döllenme Üzerindeki Etkisi . Acta Aquatica Turcica , 16 (2) , 266-274 . DOI: 10.22392/actaquatr.660155