Chromomycin A3 and DAPI Staining of Chromosomes of Three Endemic Pseudophoxinus Bleeker, 1860 (Teleostei: Leuciscidae) Species from Anatolia

Year 2020, Volume: 16 Issue: 2, 283 - 289, 01.06.2020

Muradiye Karasu Ayata

https://doi.org/10.22392/actaquatr.667595

Cited By: 1

Abstract

The karyotypes and other chromosomal markers of representatives of the genus Pseudophoxinus have been little studied. Therefore, this study documents chromosomal NOR phenotypes as revealed using Chromomycin A3 (CMA3) and DAPI chromosomal stainings in three species Pseudophoxinus elizavetae Bogutskaya, Küçük & Atalay, 2007, P. firati Bogutskaya, Küçük & Atalay, 2007 and P. hittitorum Freyhof & Özuluğ, 2010, endemic in Anatolia. Simple NOR phenotype with only two CMA3 positive signals were observed in the karyotype of P. firati whereas derived NOR phenotype with four CMA3 positive signals were observed in those of P. elizavetae and P. hittitorum, respectively. No DAPI positive signal was detected in karyotypes in all three species. This study described new NOR phenotypes, i.e. number and position of major rDNA genes, in genomes of three Pseudophoxinus species contributing thus to known diversity of NOR phenotypes in otherwise karyotypically highly conservative leuciscid fishes.

Keywords

Fish cytotaxonomy, chromosome banding, chromosomal markers, major rDNA genes

Supporting Institution

Kırşehir Ahi Evran University

Project Number

SYO.A4.18.001

Thanks

This study was supported by Kırşehir Ahi Evran University Scientific Research Projects (Project code: SYO.A4.18.001).

Anadolu’da Yaşayan Endemik Üç Pseudophoxinus Bleeker, 1860 (Teleostei: Leuciscidae) Türünün CMA3 ve DAPI Boyalı Kromozomları

Abstract

Pseudophoxinus cinsindeki balıkların kromozom ve diğer sitotaksonomik markerları konusundaki çalışma sayısı azdır. Bu çalışmada, Anadolu’ya endemik üç leuciscine türü Pseudophoxinus elizavetae Bogutskaya, Küçük & Atalay, 2007, P. firati Bogutskaya, Küçük & Atalay, 2007 ve P. hittitorum Freyhof & Özuluğ, 2010’da kromozomal NOR fenotipleri CMA3 ve DAPI kromozomal boyama ile çalışılmıştır. P. firati’nin karyotipinde iki, P. elizavetae ve P. hittitorum’un karyotiplerinde ise dört CMA3 pozitif sinyal gözlenmiştir. Üç türün karyotipinde de DAPI pozitif sinyal gözlenmemiştir. Bu çalışma üç Pseudophoxinus türünün genomunda majör rDNA genlerinin sayısı ve pozisyonu gibi yeni NOR fenotiplerini tanımlamıştır. Böylece karyotipik olarak yüksek derecede konservatif bilinen leuciscid NOR fenotip çeşitliliğine katkıda bulunur.

Keywords

Balık sitotaksonomisi, kromozomal bantlama, kromozom markerları, majör rDNA genleri

Project Number

SYO.A4.18.001

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