Quercetin'in HT-29 ve HCT-116 kolon kanseri hücre hatları üzerine etkisinin RIPK1, RIPK3 ve MLKL genlerinin ekspresyonları ile incelenmesi

Year 2023, , 182 - 187, 31.12.2023

Alp Can Tuncer , Sevval Has , Haydar Bağış , Esra Bozgeyik

https://doi.org/10.30569/adiyamansaglik.1302585

Abstract

Amaç: Quercetin kolon kanseri dahil birçok kanser çeşidinde anti-kanser aktivite gösteren bir bileşiktir. Ancak, quercetinin nekroptoz yolağı üzerine etkilerini gösteren çalışmalar kısıtlıdır ve bu nedenle bu çalışmada quercetinin nekroptoz yolağına etkisinin belirlenmesi amaçlanmıştır.
Gereç ve Yöntem: HT-29 ve HCT-116 kolon kanseri hücreleri kültür edilip farklı konsantrasyondaki quercetinin hücre canlılığına etkisi MTT yöntemi ile belirlendi. Sonrasında quercetinin nekroptoza etkisinin belirlenmesi için RIPK1, RIPK3 ve MLKL genlerinin ekspresyon seviyesi analiz edildi.
Bulgular: HT-29 hücrelerinde quercetinin aktif dozu 50 µM (p=0,0286) olarak bulunurken HCT-116 hücrelerinde 100 µM (p=0,009) bulundu. 50 ve 100 µM quercetin ile maruz bırakılan HT-29 hücrelerinde nekroptoz belirteçlerinin ekspresyon seviyesinde ciddi bir artış tespit edildi.
Sonuç: Bu çalışmanın sonuçları quercetinin nekroptoz yolağının aktif bir düzenleyicisi olabileceğini göstermiştir.

Keywords

Kolon kanseri, MLKL, Nekroptoz, Quercetin, RIPK1, RIPK3

Ethical Statement

Bu çalışma için etik kurul onayı etik kurul izni alınmamıştır.

Supporting Institution

Bu çalışma, 2209-A Üniversite Öğrencileri Araştırma Projeleri Destekleme Programı kapsamında Adıyaman Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Laboratuvar Teknikleri Programı öğrencisi Alp Can TUNCER’in 1919B012202294 numaralı projesi ile Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK) tarafından desteklenmiştir.

Project Number

1919B012202294

The investigation of the effect of quercetin on HT-29 and HCT-116 colon cancer cell lines through the expression of RIPK1, RIPK3, and MLKL genes

Abstract

Aim: Quercetin is a compound with anti-cancer activity in many types of cancer. However, studies showing the effects of quercetin on the necroptosis pathway are limited, and therefore, the aim of this study was to determine the effect of quercetin on the necroptosis pathway.
Materials and Methods: Colon cancer cells were cultured and effect of different concentrations of quercetin on cell viability was determined by MTT method. Afterwards, the expression level of RIPK1, RIPK3 and MLKL genes were analyzed to determine the effect of quercetin on necroptosis.
Results: The active dose of quercetin was found to be 50 µM in HT-29 cells (p=0.0286), while 100 µM was found in HCT-116 cells (p=0.009). A significant increase in the expression level of necroptosis markers was detected in HT-29 cells treated with 50 and 100 µM quercetin.
Conclusion: The results of this study showed that quercetin may be an active regulator of the necroptosis pathway.

Keywords

Colon cancer, MLKL, Necroptosis, Quercetin, RIPK1, RIPK3

Ethical Statement

Bu çalışma için etik kurul onayı etik kurul izni alınmamıştır.

Supporting Institution

Bu çalışma, 2209-A Üniversite Öğrencileri Araştırma Projeleri Destekleme Programı kapsamında Adıyaman Üniversitesi, Sağlık Hizmetleri Meslek Yüksekokulu, Tıbbi Laboratuvar Teknikleri Programı öğrencisi Alp Can TUNCER’in 1919B012202294 numaralı projesi ile Türkiye Bilimsel ve Teknolojik Araştırma Kurumu (TÜBİTAK) tarafından desteklenmiştir.

Project Number

1919B012202294

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