Preliminary in vitro assessment of cytotoxic and genotoxic effects of avocado (Persea Americano) oil in breast cancer cell line (MCF-7)

Year 2023, , 162 - 168, 31.12.2023

Ayça Aktaş Şüküroğlu

https://doi.org/10.30569/adiyamansaglik.1332125

Abstract

Aim: Toxicological evaluation is required to understand the safety of avocado (Persea Americana) oil for use as a food supplement. In this study, cytotoxic and genotoxic effects of avocado oil in MCF-7 cell line were evaluated.
Materials and Methods: In this study, the MCF-7 was exposed to avocado oil (1, 10, 25 and 100 ppm) for 24, 48 and 72 hrs to assess the cytotoxic and genotoxic effects.
Results: IC50 of avocado oil were found to be 68.1, 62.8 and 64.3 ppm for 24, 48 and 72 hrs, respectively. There was a statistically significant decrease in cell polferation between the control and exposed groups (p<0.05). Micronucleus frequency was significantly increased compared with negative control (p<0.005).
Conclusion: Results of the study, avocado oil had cytotoxic and genotoxic effects in a time and concentration dependent manner. Regular use of avocado oil as a dietary supplement has been shown to have a protective effect.

Keywords

Avocado oil, Cytotoxicity, Genotoxicity, MCF-7, Cytokinesis-block micronucleus assay

Ethical Statement

In the current work, there was no animal or human experiments conducted

Supporting Institution

The authors declared that this study has received no financial support.

Project Number

Proje Numarası Bulunmamaktadır.

Thanks

Thanks to Dr. Asena Ayca Ozdemir (Mersin University Faculty of Medicine, Department of Biostatistics and Medical Informatics) for contributing to the statistical analysis.

Avokado (Persea Americano) yağının meme kanseri hücre hattı (MCF-7) üzerindeki sitotoksik ve genotoksik etkilerinin in vitro ön değerlendirmesi

Abstract

Amaç: Avokado (Persea Americana) yağının gıda takviyesi olarak kullanımında güvenliğinin anlaşılması için toksikolojik değerlendirilme yapılması gerekmektedir. Planlanan bu çalışmada avokado yağının MCF-7 hücre hattındaki sitotoksik ve genotoksik etkileri değerlendirilmiştir.
Gereç ve Yöntem: Bu çalışmada MCF-7 hücre hattı, avokado yağına (1, 10, 25 ve 100 ppm) ile 24, 48 ve 72 maruz bırakılarak sitotoksik ve genotoksik etkisi değerlendirilmiştir.
Bulgular: Avokado yağının IC50 değerleri 24, 48 ve 72 saat için sırasıyla; 68.1, 62.8 ve 64.3 ppm olarak bulunmuştur. Avokado yağının bütün maruziyet sürelerinde kontrol grubu ile maruziyet grupları arasında hücre poliferasyonundaki azalma istatiksel olarak anlamlı bulunmuştur (p<0,05). Avokado yağına maruziyetine bağlı mikroçekirdek frekansında, tüm dozlarda negatif kontrole göre önemli artış görülmüştür (p<0,005).
Sonuç: Çalışmanın sonucunda avokado yağının MCF-7 hücre hattında zamana ve konstrasyona bağımlı olarak sitotoksik ve genotoksik etkilerinin olduğu görülmüştür. Avokado yağının gıda takviyesi olarak düzenli kullanılması sonucunda koruyucu etkisinin olabileceği görülmüştür.

Keywords

Avokado yağı, Sitotoksisite, Genotoksisite, MCF-7, Sitokinezin durdurulduğu mikroçekirdek yöntemi

Ethical Statement

In the current work, there was no animal or human experiments conducted.

Supporting Institution

The authors declared that this study has received no financial support.

Project Number

Proje Numarası Bulunmamaktadır.

Thanks

Thanks to Dr. Asena Ayca Ozdemir (Mersin University Faculty of Medicine, Department of Biostatistics and Medical Informatics) for contributing to the statistical analysis.

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