Investigation of Histochemical and Histometric Distribution of Lyphocytes in Thymus and Blood Tissues Due to Rats Age

Year 2018, , 900 - 920, 15.08.2018

Alper Yalçın , Mehmet Kanter

https://doi.org/10.30569/adiyamansaglik.441354

Abstract

Objective: This study was performed to investigate the histochemical and
histometric distribution of lymphocytes in thymus and blood tissues of the rats
depending on their different age stages

Material and Method:  In the study, 60 Wistar
albino rats were used. Thymus and blood tissue samples were taken following
decapitation. Thymus tissues were firstly placed in formal-sucrose and then
Holtz solutions for fixation. Blood samples were put into the heparinized
tubes. To determine the alpha naptyhl acetate esterase  (ANAE) positive lymphocytes from the both
tissues, thymus tissue sections cut into 10 µ thickness by cryostat microtome
and smears made from blood samples were stained in the incubation solutions for
5 minutes and  3  hours 
at  pH  5.8, 
respectively.

.Results: When the ANAE  (+)  positive
lymphocytes existence in cortical and medullar parts of the thymic tissue were
evaluated for each stage statistically; the most numbers in puberty and the
least in adult stage were found respectively whereas the pre-puberty stage were
found to have much more positive lymphocytes compared with the adult stage. In
the blood tissue samples, the numbers of ANAE positive lymphocytes were found
to be very few in pre-puberty stage, the most in the puberty stage; however,
the numbers of the cells in the adult stage were fewer than puberty but more
than pre-puberty stages. The ANAE negative lymphocytes in the blood tissue
samples were regarded as B lymphocytes

Conclusion: While the rate of lymphocytes in pre-puberty and puberty stages
in blood samples paralleled with their distribution in the thymic tissue, the
rate of positive lymphocytes in peripheral blood was much more higher than
thymus tissue only in the adult stage, which it could be postulated that
abundant numbers might have been because of thymus involution or constant
proliferation of the memory T cells in periphery.

Keywords

Age, ANAE, histochemistry, lymphocytes, rat

Sıçanlarda Yaşa Bağlı Olarak Timus ve Periferal Kan Dokusunda bulunan Lenfositlerin Histokimyasal ve Histometrik İncelenmesi

Abstract

Amaç: Bu çalışma, sıçanlarda yaşa bağlı olarak
timus ve periferal kan dokusunda bulunan lenfositlerin histokimyasal ve histometrik
incelenmesi amacıyla yapıldı.

Gereç ve Yöntem: Çalışmada 60 Wistar Albino sıçan kullanıldı.  Farklı yaş grubundaki sıçanlar dekapite
edilerek timus ve kan dokuları uzaklaştırıldı. Timus dokuları sırasıyla
formol-sukroz ve Holtz solüsyonunda tespit edildi. Kan dokuları ise heparinli
tüplere alındı. Alfa naftil asetat esteraz 
(ANAE) pozitif lenfositleri belirlemek amacıyla kriyostatta timus dokusundan
alınan kesitler, pH 5.8'de 5 dakika, kan dokusundan yapılan frotiler ise pH 5.8
'de 3 saat süre ile inkübasyon solüsyonunda boyandı.

Bulgular: Timusta her dönem için korteks ve
medullada bulunan toplam ANAE (+) lenfosit dağılımı istatistiksel olarak
değerlendirildiğinde en fazla puberte, en az ergin dönemde bulunduğu;
prepuberte döneminde ise ergin döneme göre bu sayının anlamlı bir şekilde fazla
oldugu saptandı. Kan dokusunda  ise ANAE
pozitif lenfosit sayılarının prepuberte döneminde oldukça az, puberte  dönerninde 
en fazla, ergin dönemde ise puberteye göre az ancak prepuberte dönemine  göre 
fazla olduğu saptandı. Kan dokusunda ANAE negatif lenfositler B lenfosit
olarak değerlendirildi.

Sonuç: Kan ömeklerinde ANAE (+) lenfositlerin
oranı prepuberte ve puberte dönernlerinde timustaki dağılımla paralellik
gösterirken, sadece ergin dönemde periferal kandaki pozitiflik oranının timusa
göre oldukça yüksek çıkması timustaki involusyona bağlanabileceği gibi,
periferdeki bellek T hücrelerinin sürekli olarak proliferasyonu sonucu da bu
artışın olabilecegi kanaatini taşımaktayız.

Keywords

ANAE, histokimya, lenfosit, sıçan, yaş

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