Investigation of the positive effects of silymarin on valproic acid-induced liver damage in rats

Year 2019, , 1445 - 1458, 15.08.2019

İbrahim Aktaş , İlkay Armağan

https://doi.org/10.30569/adiyamansaglik.568226

Cited By: 1

Abstract

Purpose: In this study, we
evaluated the potential hepatoprotective effects of silymarin on valproic
acid-induced liver injury by histological and biochemical parameters in rat
liver.

Method:Experimental procedures
were performed on 21 male Sprague Dawley rats. Rats were divided into three
groups: group 1, control; group 2, valproic acid; group 3, valproic acid +
silymarin. The groups were administered 500 mg/kg/day valproic acidand 100
mg/kg/daysilymarin for 14 days, except control group.

Results:Silymarin treatment decreased
the levels of serumgamma glutamyl transferase, alanine amino transferase, aspartate
aminotransferase and increreased serum albumin levelssignificantly (p
<0.05). In addition, increased amount of malondialdehyde and decreased
levels of glutathione with valproic acid were significantly suppressed by
silymarin in liver tissue (p <0.05). The combination of silymarinwith valproic
acid reduced loss of body weight in the present study. Histologically, the
extent of liver damage was significantly lower in the valproic acid+silymarin
group (p<0.005). Oxidative stress was decreased, antioxidant activity was
increased and histopathological changes were reduced in the valproic acid + silymarin
group compared to the valproic acid group.

Conclusion:This study revealed that
the liver injury induced by valproic acid was attenuated with silymarin
administration. Silymarincan protect rat liver against valproic acid induced
injury by its anti-oxidative effect,
and might be useful for reducing the severity of liver injury.

Keywords

valproic acid, silymarin, liver injury, rat

Silimarinin, ratlarda valproik asitin indüklediği karaciğer hasarı üzerine olumlu etkilerinin araştırılması

Abstract

Amaç:Bu çalışmada; valproik asit kaynaklı ratlarda
karaciğer hasarına karşı silimarin'in olası hepatoprotektif etkilerini
histolojik ve biyokimyasal değerlendirmeler kullanarak araştırmayı amaçladık.

Gereç ve yöntemler:Deneysel işlemler, 21 adet Sprague
Dawley sıçanı üzerinde gerçekleştirildi. Sıçanlar üç gruba ayrıldı: grup 1,
kontrol; grup 2, valproik asit; grup 3, valproik asit + silimarin. Gruplara,
kontrol grubu hariç 500 mg/kg/gün valproik asit ve 100 mg/kg/gün silimarin 14
gün  uygulandı.

Bulgular:Valproik asitile artan serum gama glutamil transferaz,
alanin amino transferaz, aspartat aminotransferaz ve azalan serum albumin
seviyesi silimarin tedavisi ile tersine çevrildi (p<0.05). Ek olarak,
karaciğer dokusunda valproik asit ile malondialdehit seviyesinin artması ve glutatyon
seviyesinin azalması, silimarin tarafından önemli ölçüde baskılandı (p<0.05).
Silimarin'in,valproik asit ile kombinasyonu bu çalışmada vücut ağırlığı kaybını
azaltmıştır. Histolojik olarak, valproik asit + silimarin grubunda karaciğer
hasarının derecesi anlamlı olarak dah düşük bulundu (p <0.005). Valproik
asit + silimarin grubunda; valproik asit grubuna göre oksidatif streste azalma,
antioksidan aktivitede artışve histopatolojik değişikliklerde azalma kaydedildi.

Sonuç:Bu çalışma valproik asit'in indüklediği karaciğer
hasarının silimarin uygulaması ile azaldığını ortaya konuldu. Silimarin, sıçan
karaciğerini anti-oksidatif etkisi ile valproik asit kaynaklı hasara karşı
koruyabilir ve karaciğer hasarının boyutunu azaltmak için faydalı olabilir.

Keywords

Valproroik asit, silmarin, karaciğer, sıçan

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