Aspartam ve asesülfam K kullanımının testis yapısına etkilerinin ince yapı düzeyinde incelenmesi

Year 2020, Volume: 6 Issue: 3, 320 - 331, 03.12.2020

Leman Sencar Yurdun Kuyucu

https://doi.org/10.30569/adiyamansaglik.789927

Abstract

Amaç: Bu çalışma, ülkemizde ve dünyada yaygın olarak kullanılan aspartam ve asesülfam K’nın, sıçanlarda testis dokuları üzerindeki etkilerinin ışık ve elektron mikroskobik düzeyde araştırılması amacıyla yapılmıştır.
Gereç ve Yöntem: Çalışmada 60 adet erkek sıçan kullanıldı ve bu denekler rastgele olarak 6 eşit gruba ayrıldı. 1. gruptaki hayvanlar kontrol grubu olarak değerlendirildi; 2.gruptaki hayvanlara 200 mg/kg/gün aspartam; 3.gruptaki hayvanlara 300 mg/kg/gün aspartam; 4. gruptaki hayvanlara 300 mg/kg/gün asesülfam K; 5. gruptaki hayvanlara 600 mg/kg/gün asesülfam K ve son gruba da 300 mg/kg/gün aspartam+300 mg/kg/gün asesülfam K birlikte 8 hafta süreyle verildi. 8. haftanın sonunda deneklerden testis dokuları alındı ve dokular ışık ve elektron mikroskobik olarak değerlendirildi.
Bulgular: Aspartam ve asesülfam K kullanımının testis dokusunda dejenerasyona yol açtığı ve spermatogenez sürecinde sağlıklı spermatid oluşumunu engellediği izlendi.
Sonuç: Aspartam ve asesülfam K tüketiminin erkek sıçanlarda testis dejenerasyonuna neden olduğu görüldü. Bununla birlikte, erkek üreme sistemindeki aspartam ve asesülfam K toksisitesinin doğrulanması için daha kapsamlı çalışmaların yapılması gerektiği kanaatine varıldı.

Keywords

Asesülfam K, Aspartam, Elektron Mikroskop, Işık Mikroskop, Testis

Ultrastructural examination of the effects aspartame and acesulfame K on testis structure

Abstract

Aim: The present study was carried out to investigate the effects of aspartame and acesulfame K, which are widely used in our country and the world, on testicular tissues in rats at light and electron microscopic level.
Materials and Methods: In this experimental study, 60 male rats were used. The experimental animals were randomly divided into 6 equal groups. Animals in group 1 were considered as control group; Animals in group 2 received 200 mg/kg/day of aspartame; Group 3 animals received 300 mg/kg/day aspartame; Animals in group 4 received acesulfame K at 300 mg kg/day; The animals in the 5th group were given acesulfame K 600 mg/kg/day and the last group received 300 mg/kg/day aspartame+300 mg/kg/day acesulfame K for 8 weeks. At the end of the 8th week, testicular tissue samples obtained from rats were examined by light and electron microscopic methods.
Results: It has been observed that the use of aspartame and acesulfame K causes degeneration in the testicular tissue depending on the döşe and it has been observed that prevents the formation of healthy spermatids during the spermatogenesis process.
Conclusion: The findings of the present study elucidated that consumption of aspartame and acesulfame K resulted in testis damages in mice. Confirmation of the toxicity of aspartame in male reproductive system requires more extensive experimental studies and clinical trials.

Keywords

Acesulfame K, Aspartame, Electron microscope, Light microscope, Testis

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