The musculoprotective effects of thymoquinone on ameliorating soleus muscle damage induced by valproic acid in rats

Year 2022, Volume 8, Issue 3, 170 - 180, 31.12.2022

Sebile AZİRAK Deniz TAŞTEMİR KORKMAZ Sedat BİLGİÇ Murat SEVİMLİ Mehmet Kaya ÖZER

https://doi.org/10.30569/adiyamansaglik.1202066

Abstract

Aim: To determine the potential musculoprotective effects of thymoquinone (TQ) on valproic acid (VPA)-induced muscle damage.

Materials and Methods: Twenty-one male Spraque-Dawley rats were randomly separated into 3 groups (n = 7): Control, VPA, VPA + TQ. Oral VPA (500 mg/kg/day) and TQ (50 mg/kg/day) were given to the rats for a period of 14 days. On the 15th day, soleus muscle tissues were taken for evaluating the expression levels of the Alpha-actinin-3 (ACTN3) and Myosin heavy chain 7 (MYH7) genes and histological analysis.

Results: The VPA + TQ group showed significantly higher ACTN3 and lower MYH7 gene expression, and decreased NADPH oxidase-4 (NOX4) and caspase-3 (CAS-3) levels than the VPA group. Also, histopathological changes were decreased in the VPA + TQ group in comparison with the VPA group.

Conclusion: VPA-induced soleus muscle damage was alleviated due to the antioxidant and antiapoptotic effects of TQ. TQ may be beneficial in treating soleus muscle damage caused by VPA.

Keywords

Thymoquinone, Valproic acid, Apoptosis, Toxicity, Soleus muscle damage

Sıçanlarda valproik asidin neden olduğu soleus kas hasarını iyileştirmede timokinonun koruyucu etkileri

Abstract

Amaç: Çalışmada timokinonun (TQ) valproik asit (VPA) kaynaklı kas hasarı üzerindeki potansiyel koruyucu etkilerinin belirlenmesi amaçlanmıştır.

Gereç ve Yöntem: Yirmi bir erkek Spraque-Dawley sıçan rastgele 3 gruba ayrıldı (n = 7): Kontrol, VPA, VPA + TQ. Sıçanlara 14 gün süreyle oral VPA (500 mg/kg/gün) ve TQ (50 mg/kg/gün) verildi. Onbeşinci günde, Alfa-aktinin-3 (ACTN3) ve Miyozin ağır zincir 7 (MYH7) genlerinin ekspresyon düzeylerinin belirlenmesi ve histolojik analiz için soleus kas dokuları alındı.

Bulgular: VPA + TQ grubunda, VPA grubuna göre önemli ölçüde daha yüksek ACTN3 ve daha düşük MYH7 gen ekspresyonu ile düşük NADPH oksidaz-4 (NOX4) ve kaspaz-3 (CAS-3) seviyeleri gözlendi. Ayrıca VPA + TQ grubunda VPA grubuna göre histopatolojik değişikliklerin azaldığı da görüldü.

Sonuç: TQ'un antioksidan ve antiapoptotik etkileri nedeniyle VPA kaynaklı soleus kas hasarını hafiflettiği ve kas hasarını tedavi etmede faydalı olabileceği sonucuna varılmıştır.

Keywords

Timokinon, Valproik asit, Apoptoz, Toksisite, Soleus kas hasarı

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