The effect of Thymus vulgaris essential oil on the formation of Candida albicans biofilm on denture base materials: An in vitro study

Year 2024, Volume: 10 Issue: 2, 97 - 105, 30.08.2024

Leyla Güven , Nurdan Polat Sağsöz , Figen Orhan , Neslihan Macit Kavaz

https://doi.org/10.30569/adiyamansaglik.1457922

Abstract

Aim: This study aims to evaluate the antifungal properties of Thymus vulgaris (thyme) essential oil (TVEO) on Candida albicans-infested polymethyl methacrylate (PMMA) denture base materials and identify the volatile components of T. vulgaris essential oil.
Materials and Methods: The investigation involved establishing C. albicans's presence on resin surfaces manufactured in 1x1x0.1 cm size. The resin surfaces on which the C. albicans biofilm had formed were then soaked in a 2% and 5% solution of TVEO and the effect on the amount of C. albicans colonisation were evaluated. The volatile components of TVEO were determined using the GC-MS method.
Results: Solutions prepared from 2% and 5% TVEO showed better antifungal activity than Corega. The major components detected by GC-MS in the TVEO were carvone 61.36%, linalool 8.32%.
Conclusion: TVEO, showed significant antifungal effects on PMMA resin surfaces. This oil can, therefore, be recommended as an inexpensive, uncomplicated and efficient natural cleaning agent for those wearing dentures.

Keywords

Antifungal activity, C. albicans, Denture cleansers, Thymus vulgaris

Ethical Statement

There was no data obtained from animal or human experiments for this article.

Supporting Institution

The authors received no financial support for the research.

Thanks

We would like to thank Prof. Dr. Özlem Barış for the laboratory facilities.

Thymus vulgaris esansiyel yağının protez kaide materyalleri üzerinde Candida albicans biyofilm oluşumuna etkisi: İn vitro bir çalışma

Abstract

Amaç: Bu çalışmanın amacı Candida albicans tutulumu sağlanmış polimetil metakrilat (PMMA) protez kaide materyallerine Thymus vulgaris (kekik) esansiyel yağının (TVEO) antifungal etkisini değerlendirmek ve TVEO’nun uçucu bileşenlerini belirlemektir.
Gereç ve Yöntem: Çalışmada ilk olarak 1x1x0,1 cm boyutunda hazırlanan rezin yüzeylerine C. albicans tutulumu sağlandı. Daha sonra rezin yüzeyleri, TVEO’dan hazırlanan %2 ve %5 oranındaki solüsyonunda bekletilerek C. albicans kolonizasyonundaki değişiklik değerlendirildi. TVEO’nun uçucu bileşenleri GC-MS yöntemi ile belirlendi.
Bulgular: TVEO’dan %2 ve %5 oranında hazırlanan solüsyonlar Corega’ya göre daha iyi antifungal aktivite gösterdi. TVEO’da GC-MS ile tespit edilen majör bileşenler carvone %61,36, linalol %8,32.
Sonuç: TVEO, PMMA rezin yüzeylerinde etkili antifungal aktivite gösterdi. Protez kullanıcıları için alternatif, ucuz, basit ve etkili bir doğal temizleyici olarak önerilebilir.

Keywords

Antifungal aktivite, C. Albicans, Protez temizleyiciler, Thymus vulgaris

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