Evaluation of Surface Roughness and Bacterial Adhesion After Different Finishing Procedures on CAD/CAM Ceramic Materials
Year 2024,
Volume: 10 Issue: 1, 23 - 34, 17.04.2024
Mahmut Erçil
,
Özge Parlar Öz
,
Yasemin Zer
,
Ayşe Büyüktaş Manay
Abstract
Objectives: The aim of this study is to determine the ideal finishing process for CAD/CAM ceramic materials for use in dental restorations. For this determination, the surface roughness and bacterial adhesion counts of the ceramic surfaces after common finishing procedures are compared.
Materials and Methods: A total of 120 samples with a thickness of 1 ± 0.05 mm were obtained from different CAD/CAM ceramic materials (IPS Empress CAD, IPS E-Max CAD, VITA Suprinity and CEREC blocs). The samples from each ceramic group were divided into three subgroups (n = 10) according to the finishing procedure (control, manual polished and glazed). The surface roughness values (Ra) were measured with an optical profilometer before and after each finishing procedure. An additional sample from each group was prepared for scanning, and surface roughness was visualized using scanning electron microscope analysis. A bacterial adhesion test was applied to determine the levels of Streptococcus mutans adhesion on each surface.
Results: After the finishing process, the highest Ra value was observed in the LC glazed group, and a statistically significant difference was found between the LC glazed group and the manual polished groups (p < 0.05). The least amount of bacterial adhesion was observed in the LD glaze group. There were no statistically significant differences between the LD glaze group and the LC and ZL control groups (p > 0.05).
Conclusion: The polished samples had smoother ceramic surfaces than the glazed samples. When evaluated in terms of bacterial adhesion, the effective polishing method for each ceramic type varied. Therefore, the polishing method should be chosen according to ceramic type.
Ethical Statement
Ethics committee approval was received for this study from the clinical researches ethics committee of Gaziantep University. (Decision number: 2019/509)
Supporting Institution
Gaziantep University Scientific Research Projects Governing Unit supported this study. (Project No: DHF.UT.20.04)
Project Number
DHF.UT.20.04
Thanks
The authors thank to Prof. Dr. Seval KUL for statistical analysis of the study.
References
- Kelly JR, Nishimura I, Campbell SD. Ceramics in
dentistry: Historical roots and current perspectives. J
Prosthet Dent 1996; 75: 18-32.
- Coldea A, Swain MV, Thiel N. Mechanical properties
of polymer-infiltrated-ceramic- network materials. Dent
Mater 2013; 29: 419-26.
- Kirsch C, Ender A, Attin T, Mehl A. Trueness of four
different milling procedures used in dental CAD / CAM
systems. Clin Oral Invest 2017; 21: 551-8.
- Giordano R. Materials for chairside CAD/CAMproduced
restorations. J Am Dent Assoc 2006;137:14S-21S.
- Wildgoose DG, Johnson A, Winstanley RB. Glass/
ceramic/refractory techniques, their development and
introduction into dentistry: A historical literature review.
J Prosthet Dent 2004; 91:136-43.
- Sannino G, Germano F, Arcuri L, Bigelli E, Arcuri C,
Barlattani A. CEREC CAD/CAM Chairside System. Oral
Implantol (Rome) 2015; 7: 57-70.
- Sieper K, Wille S, Kern M. Fracture strength of lithium
disilicate crowns compared to polymer-infiltrated ceramicnetwork
and zirconia reinforced lithium silicate crowns. J
Mech Behav Biomed Mater 2017; 74: 342-348.
- Carrabba M, Vichi A, Vultaggio G, Pallari S, Paravina
R, Ferrari M. Effect of Finishing and Polishing on the
Surface Roughness and Gloss of Feldspathic Ceramic for
Chairside CAD/ CAM Systems. Oper Dent 2017; 42: 175-
184.
- Schmidlin PR, Gohring TN. Finishing tooth-colored
restorations in vitro: an index of surface alteration and
finish-line destruction. Oper Dent 2004; 29: 80-6.
- Yildiz H, Sen E, Dalcik H, Meseli S.E. Evaluation of
cell morphology and adhesion capacity of human gingival
fibroblasts on titanium discs with different roughened
surfaces: an in vitro scanning electron microscope analysis
and cell culture study. Folia Morphol 2023; 82: 63-71.
- Aas JA, Paster BJ, Stokes LN, Olsen I, Dewhirst FE.
Defining the normal bacterial flora of the oral cavity. J Clin
Microbiol 2005; 43: 5721-32.
- Avila M, Ojcius DM, Yilmaz O. The oral microbiota:
Living with a permanent guest. DNA Cell Biol 2009; 28:
405-11.
- Curyl MS, Silva CB, Nogueira RD, Campos MGD,
Palma-Dibb RG, Geraldo-Martins VR. Surface roughness
and bacterial adhesion on root dentin treated with diode
laser and conventional desensitizing agents. Lasers Med
Sci 2018; 33: 257-62.
- Aboushelib MN, Elsafi MH. Survival of resin
infiltrated ceramics under influence of fatigue. Dental
Materials 2016; 32: 529–534.
- Jefferies SR. The art and science of abrasive finishing
and polishing in restorative dentistry. Dent Clin North Am
1998; 42: 613-27.
- Fasbinder DJ, Neiva GF. Surface evaluation of
polishing techniques for new resilient CAD / CAM
restorative materials. J Esthet Restor Dent 2016; 28: 56-
66.
- Han G, Kim J, Lee M, Chae S, Lee Y, Cho B.
Performance of a novel polishing rubber wheel in
improving surface roughness of feldspathic porcelain.
Dent Mater J 2014; 33: 739-748.
- Tekce N, Fidan S, Tuncer S, Kara D, Demirci M. The
effect of glazing and aging on the surface properties of
CAD / CAM resin blocks. J Adv Prosthodont 2018; 10:
50-7.
- El Zohairy AA, De Gee AJ, Mohsen MM, Feilzer AJ.
Microtensile bond strength testing of luting cements to
prefabricated CAD / CAM ceramic and composite blocks.
Dent Mater 2003; 19: 575-83.
- Whitehead SA, Shearer AC, Watts DC, Wilson NH.
Comparison of two stylus methods for measuring surface
texture. Dent Mater 1999; 15: 79-86.
- Kakaboura A, Fragouli M, Rahiotis C, Silikas N.
Evaluation of surface characteristics of dental composites
using profilometry, scanning electron, atomic force
microscopy and gloss- meter. J Mater Sci Mater Med
2007; 18: 155-63.
- Janus J, Fauxpoint G, Arntz Y, Pelletier H, Etienne
O. Surface roughness and morphology of three
nanocomposites after two different polishing treatments
by a multitechnique approach. Dent Mater 2010; 26: 416-
25.
- Heintze SD, Forjanic M, Ohmiti K, Rousson V.
Surface deterioration of dental materials after simulated
toothbrushing in relation to brushing time and load. Dent
Mater 2010; 26: 306-19.
- Hulterstrom AK, Bergman M. Polishing systems for
dental ceramics. Acta Odontol Scand 1993; 5: 229-34.
- Sarac D, Sarac YS, Yuzbasioglu E, Bal S. The effects
of porcelain polishing systems on the color and surface
texture of feldspathic porcelain. J Prosthet Dent 2006; 96:
122-8
- Aravind P, Razak PA, Francis PG, Issac JK, Shanoj RP,
Sasikumar TP. Comparative Evaluation of the Efficiency of Four Ceramic Finishing Systems. J I Oral Health 2013; 5: 59-64.
- Flury S, Lussi A, Zimmerli B. Performance of different
polishing techniques for direct CAD / CAM ceramic
restorations. Oper Dent 2010; 35: 470-81.
- Han GJ, Kim JH, Lee M, Chae SY, Lee YH, Cho
BH. Performance of a novel polishing rubber wheel in
improving surface roughness of feldspathic porcelain.
Dent Mater J 2014; 33:739-748.
- Albakrya M, Guazzatoa M, Swain MV. Effect of
sandblasting, grinding, polishing and glazing on the
flexural strength of two pressable all-ceramic dental
materials. J Dent 2004; 32: 91-99.
- Gonuldas F, Ozturk C, Atalay P, Oztas D. Influence
of different surface finishing techniques on machinable
feldspathic and leucite-reinforced ceramics. Dent Mater
J 2019; 38: 317-322.
- Sulik WD, Plekavich EJ. Surface fi nishing of dental
porcelain. J Prosthet Dent 1981; 46: 217-221.
- Mohammadibassi M, Rezvani MB, Golzari H, Salehi
EM, Fahimi MA, Fard MJK. Effect of Two Polishing
Systems on Surface Roughness, Topography, and Flexural
Strength of a Monolithic Lithium Disilicate Ceramic. J
Prosthodont 2019; 28: e172-e180.
- Quirynen M, Bollen CM, Papaioannou W, Van Eldere
J, van Steenberghe D. The influence of titanium abutment
surface roughness on plaque accumulation and gingivitis:
short-term observations. Int J Oral Maxillofac Implants
1996; 11: 169-78.
- Bollen CM, Papaioanno W, Van Eldere J, Schepers
E, Quirynen M, van Steenberghe D. The influence of
abutment surface roughness on plaque accumulation and
peri-implant mucositis. Clin Oral Implants Res 1996; 7:
201-11.
- Glass RT, Conrad RS, Köhler GA, Warren AJ, Bullard
JW. Microbiota found in protective athletic mouthguards.
Sports Health 2011; 3: 244-8.
- Piva AD, Contreras LPC, Ribeiro FC, Anami LC,
Camargo SEA, Jorge AOC, Bottino MA. Monolithic
Ceramics: Effect of Finishing Techniques on Surface
Properties, Bacterial Adhesion and Cell Viability. Oper
Dent 2018; 43: 315-25.
- Hahnel S, Rosentritt M, Handel G, Bürgers R. Surface
characterization of dental ceramics and initial streptococcal
adhesion in vitro. Dent Mater 2009; 25: 969-75.
- Aykent F, Yondem I, Ozyesil AG, Gunal SK, Avunduk
MC, Ozkan S. Effect of different finishing techniques for
restorative materials on surface roughness and bacterial
adhesion. J Prosthet Dent 2010; 103: 221-227.
- Song F, Koo H, Ren D. Effects of material properties
on bacterial adhesion and biofilm formation. J Den Res
2015; 94:1027-1034.
- Quirynen M, Van der Mei HC, Bollen CM, Van
den Bossche LH, Doornbusch GI, van Steenberghe D,
Busscher HJ. The influence of surface-free energy on
supra- and subgingival plaque microbiology: An in vivo
study on implants. J Periodontol 1994; 65: 162-7.
- S Kreve, AC Dos Reis. Effect of surface properties
of ceramic materials on bacterial adhesion: A systematic
review. J Esthet Restor Dent 2022; 34: 461-472.
- Aksoy G, Polat H, Polat M, Coskun G. Effect of
various treatment and glazing (coating) techniques on the
roughness and wettability of ceramic dental restorative
surfaces. Colloid Surf B Biointerfaces 2006; 53: 254-9.
- Lawaf S, Azizi A, Farzad A, Adimi P. Effect of surface
treatments of porcelain on adhesion of Candida albicans.
Gen Dent 2016; 64: e1-e4.
- Stti R, Kantorski KZ, Monaco C, Valandro LF, Ciocca
L, Bottino MA. SEM evaluation of in situ early bacterial
colonization on a Y-TZP ceramic: A pilot study. Int J
Prosthodont 2007; 20: 419-22.
- Teughels W, Van Assche N, Sliepen I, Quirynen
M. Effect of material characteristics and/or surface
topography on biofilm development. Clin Oral Implants
Res. 2006; 2: 68–81.
- Renner LD, Weibel DB. Physicochemical regulation
of biofilm formation. MRS Bull 2011; 36: 347-355.
- Lin HY, Liu YL, Wismeijer D, Crielaard W, Deng
DM. Effects of oral implant surface roughness on bacterial
biofilm formation and treatment efficacy. Inter J Oral
Maxillofacial Implants 2013; 28:1226-31.
Seramik Esaslı CAD/CAM Materyallerinde Bitim İşlemleri Sonrası Yüzey Pürüzlülüğü ve Bakteri Tutulumunun Değerlendirilmesi
Year 2024,
Volume: 10 Issue: 1, 23 - 34, 17.04.2024
Mahmut Erçil
,
Özge Parlar Öz
,
Yasemin Zer
,
Ayşe Büyüktaş Manay
Abstract
AMAÇ: Seramik malzemeler için ideal bitim işlemini yüzey pürüzlülüğü ve bakteriyel adezyon parametreleri ile değerlendirerek belirlemektir.
GEREÇ-YÖNTEM: Farklı CAD/CAM seramik malzemelerinden (IPS Empress CAD, IPS E-Max CAD, VITA Suprinity ve CEREC blokları) 1±0.05 mm kalınlığında 120 numune hazırlandı. Her seramik grubundan hazırlanan numuneler bitirme işlemine göre (kontrol, manuel polisaj ve glaze) üç alt gruba (n=10) ayrıldı. Yüzey pürüzlülük değerleri başlangıçta ve işlem sonrası olmak üzere iki kere optik profilometre cihazı kullanılarak ölçüldü. Taramalı elektron mikroskobu (SEM) analizi için her gruptan birer numune hazırlandı ve yüzey pürüzlülüğü SEM ile görüntülendi. Alınan numunelerin yüzeylerinde S. mutans tutulumu olup olmadığını belirlemek için bakteriyel adezyon testi uygulandı.
BULGULAR: Bitim işleminlerinden sonra en yüksek Ra değeri LC glaze grubunda gözlendi ve LC glaze grubu ile polisaj grupları arasında istatistiksel olarak anlamlı bir fark bulundu (p<0.05). Bakteriyel adezyon miktarı en az LD glaze grubunda gözlenmiştir. LD glaze grubu ile LC kontrol ve ZL kontrol grupları arasında istatistiksel olarak anlamlı fark bulunmamıştır (p> 0.05).
SONUÇ: Polisaj işlemi seramik yüzeyde glaze olan yüzeylere göre daha pürüzsüz alanlar bırakmıştır. Bakteriyel adezyon açısından değerlendirildiğinde her seramik türü için etkili polisaj yöntemi farklı olduğu ortaya çıkmıştır. Bu nedenle seramik cinsine göre polisaj yöntemi tercih edilmelidir.
Project Number
DHF.UT.20.04
References
- Kelly JR, Nishimura I, Campbell SD. Ceramics in
dentistry: Historical roots and current perspectives. J
Prosthet Dent 1996; 75: 18-32.
- Coldea A, Swain MV, Thiel N. Mechanical properties
of polymer-infiltrated-ceramic- network materials. Dent
Mater 2013; 29: 419-26.
- Kirsch C, Ender A, Attin T, Mehl A. Trueness of four
different milling procedures used in dental CAD / CAM
systems. Clin Oral Invest 2017; 21: 551-8.
- Giordano R. Materials for chairside CAD/CAMproduced
restorations. J Am Dent Assoc 2006;137:14S-21S.
- Wildgoose DG, Johnson A, Winstanley RB. Glass/
ceramic/refractory techniques, their development and
introduction into dentistry: A historical literature review.
J Prosthet Dent 2004; 91:136-43.
- Sannino G, Germano F, Arcuri L, Bigelli E, Arcuri C,
Barlattani A. CEREC CAD/CAM Chairside System. Oral
Implantol (Rome) 2015; 7: 57-70.
- Sieper K, Wille S, Kern M. Fracture strength of lithium
disilicate crowns compared to polymer-infiltrated ceramicnetwork
and zirconia reinforced lithium silicate crowns. J
Mech Behav Biomed Mater 2017; 74: 342-348.
- Carrabba M, Vichi A, Vultaggio G, Pallari S, Paravina
R, Ferrari M. Effect of Finishing and Polishing on the
Surface Roughness and Gloss of Feldspathic Ceramic for
Chairside CAD/ CAM Systems. Oper Dent 2017; 42: 175-
184.
- Schmidlin PR, Gohring TN. Finishing tooth-colored
restorations in vitro: an index of surface alteration and
finish-line destruction. Oper Dent 2004; 29: 80-6.
- Yildiz H, Sen E, Dalcik H, Meseli S.E. Evaluation of
cell morphology and adhesion capacity of human gingival
fibroblasts on titanium discs with different roughened
surfaces: an in vitro scanning electron microscope analysis
and cell culture study. Folia Morphol 2023; 82: 63-71.
- Aas JA, Paster BJ, Stokes LN, Olsen I, Dewhirst FE.
Defining the normal bacterial flora of the oral cavity. J Clin
Microbiol 2005; 43: 5721-32.
- Avila M, Ojcius DM, Yilmaz O. The oral microbiota:
Living with a permanent guest. DNA Cell Biol 2009; 28:
405-11.
- Curyl MS, Silva CB, Nogueira RD, Campos MGD,
Palma-Dibb RG, Geraldo-Martins VR. Surface roughness
and bacterial adhesion on root dentin treated with diode
laser and conventional desensitizing agents. Lasers Med
Sci 2018; 33: 257-62.
- Aboushelib MN, Elsafi MH. Survival of resin
infiltrated ceramics under influence of fatigue. Dental
Materials 2016; 32: 529–534.
- Jefferies SR. The art and science of abrasive finishing
and polishing in restorative dentistry. Dent Clin North Am
1998; 42: 613-27.
- Fasbinder DJ, Neiva GF. Surface evaluation of
polishing techniques for new resilient CAD / CAM
restorative materials. J Esthet Restor Dent 2016; 28: 56-
66.
- Han G, Kim J, Lee M, Chae S, Lee Y, Cho B.
Performance of a novel polishing rubber wheel in
improving surface roughness of feldspathic porcelain.
Dent Mater J 2014; 33: 739-748.
- Tekce N, Fidan S, Tuncer S, Kara D, Demirci M. The
effect of glazing and aging on the surface properties of
CAD / CAM resin blocks. J Adv Prosthodont 2018; 10:
50-7.
- El Zohairy AA, De Gee AJ, Mohsen MM, Feilzer AJ.
Microtensile bond strength testing of luting cements to
prefabricated CAD / CAM ceramic and composite blocks.
Dent Mater 2003; 19: 575-83.
- Whitehead SA, Shearer AC, Watts DC, Wilson NH.
Comparison of two stylus methods for measuring surface
texture. Dent Mater 1999; 15: 79-86.
- Kakaboura A, Fragouli M, Rahiotis C, Silikas N.
Evaluation of surface characteristics of dental composites
using profilometry, scanning electron, atomic force
microscopy and gloss- meter. J Mater Sci Mater Med
2007; 18: 155-63.
- Janus J, Fauxpoint G, Arntz Y, Pelletier H, Etienne
O. Surface roughness and morphology of three
nanocomposites after two different polishing treatments
by a multitechnique approach. Dent Mater 2010; 26: 416-
25.
- Heintze SD, Forjanic M, Ohmiti K, Rousson V.
Surface deterioration of dental materials after simulated
toothbrushing in relation to brushing time and load. Dent
Mater 2010; 26: 306-19.
- Hulterstrom AK, Bergman M. Polishing systems for
dental ceramics. Acta Odontol Scand 1993; 5: 229-34.
- Sarac D, Sarac YS, Yuzbasioglu E, Bal S. The effects
of porcelain polishing systems on the color and surface
texture of feldspathic porcelain. J Prosthet Dent 2006; 96:
122-8
- Aravind P, Razak PA, Francis PG, Issac JK, Shanoj RP,
Sasikumar TP. Comparative Evaluation of the Efficiency of Four Ceramic Finishing Systems. J I Oral Health 2013; 5: 59-64.
- Flury S, Lussi A, Zimmerli B. Performance of different
polishing techniques for direct CAD / CAM ceramic
restorations. Oper Dent 2010; 35: 470-81.
- Han GJ, Kim JH, Lee M, Chae SY, Lee YH, Cho
BH. Performance of a novel polishing rubber wheel in
improving surface roughness of feldspathic porcelain.
Dent Mater J 2014; 33:739-748.
- Albakrya M, Guazzatoa M, Swain MV. Effect of
sandblasting, grinding, polishing and glazing on the
flexural strength of two pressable all-ceramic dental
materials. J Dent 2004; 32: 91-99.
- Gonuldas F, Ozturk C, Atalay P, Oztas D. Influence
of different surface finishing techniques on machinable
feldspathic and leucite-reinforced ceramics. Dent Mater
J 2019; 38: 317-322.
- Sulik WD, Plekavich EJ. Surface fi nishing of dental
porcelain. J Prosthet Dent 1981; 46: 217-221.
- Mohammadibassi M, Rezvani MB, Golzari H, Salehi
EM, Fahimi MA, Fard MJK. Effect of Two Polishing
Systems on Surface Roughness, Topography, and Flexural
Strength of a Monolithic Lithium Disilicate Ceramic. J
Prosthodont 2019; 28: e172-e180.
- Quirynen M, Bollen CM, Papaioannou W, Van Eldere
J, van Steenberghe D. The influence of titanium abutment
surface roughness on plaque accumulation and gingivitis:
short-term observations. Int J Oral Maxillofac Implants
1996; 11: 169-78.
- Bollen CM, Papaioanno W, Van Eldere J, Schepers
E, Quirynen M, van Steenberghe D. The influence of
abutment surface roughness on plaque accumulation and
peri-implant mucositis. Clin Oral Implants Res 1996; 7:
201-11.
- Glass RT, Conrad RS, Köhler GA, Warren AJ, Bullard
JW. Microbiota found in protective athletic mouthguards.
Sports Health 2011; 3: 244-8.
- Piva AD, Contreras LPC, Ribeiro FC, Anami LC,
Camargo SEA, Jorge AOC, Bottino MA. Monolithic
Ceramics: Effect of Finishing Techniques on Surface
Properties, Bacterial Adhesion and Cell Viability. Oper
Dent 2018; 43: 315-25.
- Hahnel S, Rosentritt M, Handel G, Bürgers R. Surface
characterization of dental ceramics and initial streptococcal
adhesion in vitro. Dent Mater 2009; 25: 969-75.
- Aykent F, Yondem I, Ozyesil AG, Gunal SK, Avunduk
MC, Ozkan S. Effect of different finishing techniques for
restorative materials on surface roughness and bacterial
adhesion. J Prosthet Dent 2010; 103: 221-227.
- Song F, Koo H, Ren D. Effects of material properties
on bacterial adhesion and biofilm formation. J Den Res
2015; 94:1027-1034.
- Quirynen M, Van der Mei HC, Bollen CM, Van
den Bossche LH, Doornbusch GI, van Steenberghe D,
Busscher HJ. The influence of surface-free energy on
supra- and subgingival plaque microbiology: An in vivo
study on implants. J Periodontol 1994; 65: 162-7.
- S Kreve, AC Dos Reis. Effect of surface properties
of ceramic materials on bacterial adhesion: A systematic
review. J Esthet Restor Dent 2022; 34: 461-472.
- Aksoy G, Polat H, Polat M, Coskun G. Effect of
various treatment and glazing (coating) techniques on the
roughness and wettability of ceramic dental restorative
surfaces. Colloid Surf B Biointerfaces 2006; 53: 254-9.
- Lawaf S, Azizi A, Farzad A, Adimi P. Effect of surface
treatments of porcelain on adhesion of Candida albicans.
Gen Dent 2016; 64: e1-e4.
- Stti R, Kantorski KZ, Monaco C, Valandro LF, Ciocca
L, Bottino MA. SEM evaluation of in situ early bacterial
colonization on a Y-TZP ceramic: A pilot study. Int J
Prosthodont 2007; 20: 419-22.
- Teughels W, Van Assche N, Sliepen I, Quirynen
M. Effect of material characteristics and/or surface
topography on biofilm development. Clin Oral Implants
Res. 2006; 2: 68–81.
- Renner LD, Weibel DB. Physicochemical regulation
of biofilm formation. MRS Bull 2011; 36: 347-355.
- Lin HY, Liu YL, Wismeijer D, Crielaard W, Deng
DM. Effects of oral implant surface roughness on bacterial
biofilm formation and treatment efficacy. Inter J Oral
Maxillofacial Implants 2013; 28:1226-31.