The Effect of Hydrogen Peroxide/Colloidal-Ag on Staphylococcus aureus: A Pilot Study

Yıl 2023, , 25 - 30, 18.01.2023

Ayşe Bulut , Faik Serhat Özsoy , Gülçin Akca , Nazime Tuncay , Özgür Yıldırım Torun , Ömer Engin Bulut

https://doi.org/10.54617/adoklinikbilimler.1108062

Öz

Objective: To determine the distribution of Staphylococcus aureus (S. aureus) aerosols in the immediate environment of dental operators during routine dental treatment and to investigate the reduction in S. aureus colonization using 0.5% hydrogen peroxide/colloidal silver disinfectant were aimed.
Materials and Methods: The study was divided into two groups. Sterile distilled water was used in Group A (n = 90 Petri plates). Sterile distilled water with hydrogen peroxide/colloidal silver was used in Group B (n = 90 Petri plates). The plates were set up seperately in two directions on the head phantom corresponding to the 6 and 9 o’clock positions. Three petri plates were placed side by side up to 50 cm, 100 cm and 200 cm distances, and the experimental design were proceeded by contaminating with S. aureus (ATCC 29213). Mann Whitney U test was used for comparing to independent samples.
Results: When compared to Group A, S. aureus grown colonies on agar plates in Group B was significantly decreased in directions corresponding to the 6 and 9 o’clock positions to 50 cm, 100 cm, and 200 cm distances (p = 0.00).
Conclusion: H2O2/Colloidal-Ag can safely be used as a supportive precaution against S. aureus.

Anahtar Kelimeler

Contamination, Hydrogen peroxide/colloidal silver, Staphylococcus aureus

Hidrojen Peroksit/Kolloidal Gümüş’ün Staphylococcus aureus Üzerindeki Etkisi: Pilot Çalışma

Öz

Amaç: Mevcut çalışmanın amacı, rutin diş tedavisi sırasında diş hekimlerinin yakın çevresindeki S. aureus aerosollerinin dağılımını belirlemeyi ve hidrojen peroksit/kolloidal gümüş dezenfektanı kullanarak S. aureus kolonizasyonundaki azalmayı araştırmaktır.
Gereç ve Yöntem: Çalışma iki gruba ayrılmıştır. Grup A'da (n=90 petri kabı) steril distile su, Grup B'de (n = 90 petri kabı) hidrojen peroksit/kolloidal gümüş ilaveli steril distile su kullanılmıştır. Petri kapları, saat 6 ve 9 pozisyonlarına karşılık gelen bir kafa fantomu üzerine iki yönde ayrı ayrı yerleştirilmiştir. 50 cm, 100 cm ve 200 cm mesafelere kadar üç petri kabı yan yana yerleştirilip S. aureus (ATCC 29213) solüsyonu ile kontamine edilmiştir. Veri analizlerinde Mann Whitney U testi kullanılmıştır.
Bulgular: Grup B'de S. aureus bakteri sayısı Grup A’ya göre 50 cm, 100 cm ve 200 cm mesafelere kadar saat 6 ve 9 pozisyonlarına karşılık gelen yönlerde istatistiksel olarak anlamlı derecede azalmıştır (p = 0.00).
Sonuç: Hidrojen Peroksit/Kolloidal-Ag dental işlemlerde S. aureus'a karşı destekleyici önlem olarak güvenle kullanılabilir.

Anahtar Kelimeler

Hidrojen peroksit/kolloidal gümüş, Kontaminasyon, Staphylococcus aureus

Kaynakça

• 1. Samaranayake LP, Fakhruddin KS, Buranawat B, Panduwawala C. The efficacy of bio-aerosol reducing procedures used in dentistry: A systematic review. Acta Odontol Scand 2021;79(1):69-80.
• 2. Coulthard P. Dentistry and coronavirus (COVID-19)-moral decision-making. Br Dent J 2020;228(7):503-5.
• 3. Weissman DN, de Perio MA, Radonovich LJ Jr. COVID-19 and risks posed to personnel during endotracheal intubation. JAMA 2020;323(20):2027-28.
• 4. Leggat PA, Kedjarune U. Bacterial aerosols in the dental clinic: A review. Int Dent J 2001;51:39-44.
• 5. Zelante F, Ashcar H, Piochi BJA, Monson CA, Cunha PS. Incidence of Staphylococcus aureus in mouth and nose of healthy individuals: Checking of identity among isolated strains. Rev Saude Publica 1982;16(2):92-6.
• 6. Wood C. Controversies in cross-infection control. Br Dent J 1993;174(7):249-51.
• 7. Jacks ME. A laboratory comparison of evacuation devices on aerosol reduction. J Dent Hyg 2002;76(3):202-6.
• 8. Crawford JJ. State-of-the-art: Practical infection control in dentistry. J Am Dent Assoc 1985;110(4):629-33.
• 9. Bagg J, MacFarlane TW, Poxton IR, Smith A. Essentials of microbiology for dental students. 2006. Retrieved from https://www.nature.com/articles/4813517.
• 10. Grenier D. Quantitative analysis of bacterial aerosols in two different dental clinic environments. Appl Environ Microbiol 1995;61(8):3165-8.
• 11. Francis JS, Doherty MC, Lopatin U, Johnston CP, Sinha G, Ross T, et al. Severe community – onset pneumonia in healthy adults can methicillin-resistant Staphylococcus aureus carrying the Panton-Valentine leukocidin genes. Clin Infect Dis 2005;40(1):100-7.
• 12. Shepherd PA, Shojaei MA, Eleazer PD, Van Stewart A, Staat RH. Clearance of biofilms from dental unit waterlines through the use of hydroperoxide ion-phase transfer catalysts. Quintessence Int 2001;32(10):755-61.
• 13. Tirali RE, Akça G, Bulut OE. The effects of the hydrogen peroxide colloidal-Ag on dental unit waterlines and waste waters. SOJ Microbiol Infect Dis 2016;4(1):1-5.
• 14. Rosenberg Goldstein RE, Micallef SA, Gibbs SG, Davis JA, He X, George A, et al. Methicillin-resistant Staphylococcus aureus [MRSA] detected at four U.S. wastewater treatment plants. Environ Health Perspect 2012;120(11):1551-8.
• 15. Syzmanska J. Biofilm and dental unit waterlines. Ann Agric Environ Med 2003;10(2): 151-7.
• 16. Dewhirst FE, Chen T, Izard J, Paster BJ, Tanner ACR, Yu WH, et al. The human oral microbiome. J Bacteriol 2010;192(19):5002-17.
• 17. Zmantar T, Kouidhi B, Hentati H, Bakhrouf A. Detection of disinfectant and antibiotic resistance genes in Staphylococcus aureus isolated from the oral cavity of Tunisian children. Ann Microbiol 2012;62:123-8.
• 18. Negrini TC, Duque C, Oliveira ACM, Hebling J, Spolidorio LC, Spolidorio DMP. Staphylococcus aureus contamination in a pediatric dental clinic. J Clin Pediatr Dent 2009;34(1):13-8.
• 19. Lineback CB, Nkemngong CA, Wu ST, Li X, Teska PJ, Oliver HF. Hydrogen peroxide and sodium hypochlorite disinfectants are more effective against Staphylococcus aureus and Pseudomonas aeruginosa biofilms than quaternary ammonium compounds. Antimicrob Resist Infect Control 2018;7:1-7.
• 20. Özalp M, Bulut ÖE, Ataç AS, Ekizoğlu M, Kart D, Çelik HH, et al. The effect of hydrogen peroxide/colloidal silver on reducing the colonization and growth of heterotrophic bacteria in dental unit waterlines. Turk J Biol 2013;37:336-41.
• 21. Cebrián G, Sagarzazu N, Pagán R, Condón S, Mañas P. Development of stress resistance in Staphylococcus aureus after exposure to sublethal environmental conditions. Int J Food Microbiol 2010;140:26-33.
• 22. Fox, A. (2016, March 03). Retrieved from https://www.microbiologybook.org/Turkish-bact/bactchapter13bturk.htm.
• 23. Lee J, Zilm PS, Kidd SP. Novel research models for Staphylococcus aureus small colony variants (SCV) development: co-pathogenesis and growth rate. Front Microbiol 2020;11:1-8.