Evaluation of Periodontopathogenic Bacteria Levels of Peri-Implants in Patients with Type 2 Diabetes Mellitus

Year 2022, Volume: 11 Issue: 3, 295 - 304, 19.09.2022

Şeyma Bozkurt Doğan , Mazlum Bülent Kurtiş , Gülçin Akca , Gülay Tüter

https://doi.org/10.54617/adoklinikbilimler.1125615

Abstract

Objective: The aim of this study was to evaluate the amount of periodontal pathogens (Porphyromonas gingivalis (Pg), Aggregatibacter actinomycetemcomitans (Aa), Campylobacter rectus (Cr), Treponema denticola (Td)) in saliva, peri-implant subgingival plaque samples and from natural teeth closest to the implants in the type 2 diabetes patients
Materials and Methods: Thirteen patients with well-controlled T2DM and seven systemically healthy patients were recruited for this study. A total of 39 dental implants; 27 implants in the diabetic group and 12 implants in the control group were placed. Subgingival plaque and saliva samples were collected from the implant and natural teeth at baseline and postoperatively at 1 month, 4 months, and 7 months. Real-time polymerase chain reaction (RT-PCR) was used in the microbiological analysis.
Results: The amount of Td was found to be higher in the teeth and implants of the diabetes group than in the control group at follow-up times and the baseline value in the saliva was also higher in the diabetes group than in the control group. The Pg amounts at baseline and 7 months and the Cr amounts at baseline, 4 months, and 7 months were higher in the teeth of the control group than in the diabetes group. Pg amounts at baseline and 4 months and Cr amounts at 1 month were higher in the diabetes group than in the control group. The peri-implant amounts of Cr in the diabetes group at 1 month were higher than in the control group and its amounts were higher at 7months in the control group than in the diabetes group. The amounts of Aa around both the implant and the teeth did not differ between diabetes and control groups during the follow-up periods.
Conclusions: According to the results of the study, although some periodontal pathogenic bacteria increased in both diabetes and control groups during the follow-up periods, no infection was detected around the implant in either group.

Keywords

Dental Implant, Dental Plaque, Peri-implantitis, Periodontopathogen Bacteria, Saliva, Type 2 Diabetus Mellitus

Project Number

03/2010-13

Tip 2 Diabetli Bireylerde İmplant Çevresi Periodontopatojenik Bakteri Seviyelerinin Değerlendirilmesi

Abstract

Amaç: Bu çalışmanın amacı tip 2 diabetli bireylere uygulanmış olan dental implantlardan ve implantlara en yakın doğal dişlerden toplanmış olan subgingival plak örneklerinde ve tükürükteki Porphyromonas gingivalis (Pg), Aggregatibacter actinomycetemcomitans(Aa), Camploybacter rectus (Cr), Treponema denticola (Td) gibi periodontal patojen miktarlarını değerlendirmektir.
Gereç ve Yöntem: Çalışmaya 13 tip 2 diabet ve 7 sistemik sağlıklı birey olmak üzere toplamda 20 kişi dahil edildi. Toplam 39 diş implantı, 27 implant diabet grubuna, 12 implant kontrol grubundaki bireylere olmak üzere uygulandı. İmplant ve doğal dişlerden başlangıçta, operasyon sonrası 1. ay, 4. ay ve 7. ayda subginval plak ve tükürük örnekleri toplandı. Mikrobiyolojik analiz için real-time polimeraz zincir reaksiyonu kullanıldı (RT-PZR).
Bulgular: Td miktarı diabet grubunda hem doğal dişlerde hemde implant çevrelerinde tüm takip dönemlerinde ve tükürükteki başlangıç değeride kontrol grubuna göre yüksek bulundu. Pg miktarı ise başlangıç ve 7. ayda, Cr miktarı ise baslangıç, 4. ay ve 7. ayda kontrol grubundaki doğal dişlerde diabet grubuna göre istatistiksel olarak yüksek bulunurken, yine Pg miktarı 1. ay ve 4. ayda ve Cr miktarı ise 1. ayda diabet grubunda kontrol grubuna göre yüksek bulundu. Diabet grubunda implant çevresindeki Cr miktarı ise 1. ayda kontrol grubuna göre, 7. ayda ise kontrol grubunda diabetli gruba göre anlamlı derecede yüksek bulundu. Hem implant hemde doğal diş çevresindeki Aa miktarı ise takip dönemlerinde diabet ve kontrol grupları arasında farklılık göstermedi.
Sonuç: Çalışmanın sonuçlarına göre bazı periodontal patojen bakteriler takip dönemlerinde hem diabet hemde kontrol grubunda artış göstermiş olmakla birlikte, implant çevresinde her iki grupta da herhangi bir enfeksiyon tespit edilmedi

Keywords

DİŞ İmplant, Diş Plağı, İmplant Çevresi İltihap, Periodontopatojen bakteriler, Tip 2 Diabet, Tükürük

Supporting Institution

Gazi Üniversitesi Bilisel Araştırmalar Proje Birimi

Project Number

03/2010-13

Thanks

Bu çalışma Gazi Üniversitesi Bilimsel Araştırma Projeleri Birimi tarafından 03/2010-13 nolu proje kodu ile desteklenmiştir.

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