Deneysel Olarak Diyabet Oluşturulan Ratlarda Bazı Biyokimyal Ve Hematolojik Parametrelerin Değerlendirilmesi

Abstract

Diyabet, insülin direnci ve pankreastaki beta hücrelerinin hasarı sonucu yüksek kan glikoz seviyeleri, glikozüri ile karakterize, ileri aşamalarında lipid ve protein metabolizmasında da değişiklikler görülen bir hastalıktır. Sunulan araştırmada 33 adet Wistar Albino erkek rat kullanıldı ve çalışma grupları kontrol, diyabet, kontrol+sitrik asit tamponu olmak üzere planlandı. Tüm gruplardan glikoz kontrolü için kan örnekleri 0., 3., 6., 9., 16., 23., ve 30. günlerde kuyruktan, 4 hafta sonra ise aneztezi altında kalpten alındı. Çalışma sonunda kandan hematoloji ve serumdan biyokimyasal değerler ölçüldü. Kontrol grubuna kıyasla diyabet grubunda klorür ve CK düzeyleri azalırken (p<0,05), demir, glikoz, üre, ALT ve ALP düzeyleri diyabet grubunda artış gösterdi (p<0,05). Kontrol grubuna kıyasla, LDH düzeyleri hem diyabet hem de sitrik asit gruplarında önemli ölçüde azaldı (p<0,05) ve albümin düzeyleri hem diyabet hem de sitrik asit gruplarında önemli ölçüde arttı (p<0,05). Kontrol grubuna kıyasla sitrik asit grubunda sadece fosfat düzeyleri önemli ölçüde artmıştır (p<0,05). Kontrol grubuna kıyasla, diyabet grubunda GRAN, MON, MPV, PDW ve PLCR düzeylerindeki artış ve LMM, PLT, PCT ve PLCC düzeylerindeki azalma istatistiksel olarak anlamlıdır (p<0,05). Sonuç olarak, streptozotosin kaynaklı karaciğer aktivitesindeki değişiklikler için karaciğer histopatolojisi, böbrek parametrelerindeki değişiklikler için ise böbrek histopatolojisi yapılmalıdır. Tüm bunlara ek olarak, demir düzeylerindeki değişiklikleri daha iyi anlamak için demir ve hepsidin düzeylerindeki değişiklikleri değerlendirmek anlamlı olacaktır.

Keywords

• Diyabet
• Rat
• Biyokimyasal parametre

Evaluation of Some Biochemical and Hematological Parameters in Experimentally Induced Diabetic Rats

Abstract

Diabetes, defined by high blood sugar levels and glycosuria, is characterized by changes in insulin sensitivity, damage to the pancreas, and alterations in lipid and protein metabolism in advanced stages. In this study, 33 male Wistar albino rats were randomly divided into three groups: control, diabetes, and control+citric acid buffer. Blood samples for glucose control were taken from the rat's tail in all groups on days 0, 3, 6, 9, 16, 23, and 30, and from the heart under anesthesia after 4 weeks. Hematological and biochemical parameters were analyzed from blood and serum samples at the specified time intervals and at the end of the experiment, respectively.While chloride and CK levels decreased in the diabetes group compared to the control group (p<0.05), iron, glucose, urea, ALT, and ALP levels increased in the diabetes group (p<0.05). Compared to the control group, LDH levels decreased significantly in both the diabetes and citric acid groups (p<0.05), and albumin levels increased dramatically in both the diabetes and citric acid groups (p<0.05). Only phosphate levels increased significantly in the citric acid group compared to the control group (p<0.05).Compared to the control group, the increase in GRAN, MON, MPV, PDW, and PLCR levels and the decrease in LMM, PLT, PCT, and PLCC levels were statistically significant (p<0.05) in the diabetes group. In conclusion, liver histopathology should be performed for streptozotocin-induced changes in hepatic activity, and renal histopathology should be performed for changes in renal parameters. In addition to all these, it would be meaningful to evaluate the changes in iron and hepcidin levels to better understand the changes in iron levels better.

Keywords

• Diabetes
• Rat
• Biochemical parameter.

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