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Distribution of Microorganisms That Cause Otitis Externa in Dogs and Determination of Antibiotic Susceptibilities

Year 2019, Volume: 8 Issue: 1, 638 - 641, 01.01.2019

Abstract

Background/Aim: Otitis externa is the most common ear disease of dogs. Especially, various pathogenic microorganisms are frequently encountered with chronic otitis externa. In this study, it was aimed to determine the microorganism species isolated from various clinical samples, and to investigate the antibiotic susceptibility of these isolates. Materials and Methods: In this study, 22 swab specimens taken from dogs with chronic otitis externa were used. Swabs were first inoculated in 7% Sheep Blood agar, MacConkey agar and Saboraud Dextrose agar plates and the microorganisms that were growth were determined according to conventional methods. Antibiotic susceptibilities were evaluated using Disk diffusion method. Microorganism growth was observed in 22 of the samples. The samples presented 64% single and 36% multiple growth. In the study, Coagulase Negative Staphylococcus sp. 59% was the most common isolate, followed by Pseudomonas sp. 18% , Staphylococcus aureus 9% , Corynebacterium sp. 9% and Streptococcus sp. 5% . The fungal agents isolated from these 22 specimens were Candida sp. 14%, Aspergillus sp. 9% and Trichophyton sp. 5%, respectively. Conclusion: When all bacteria were taken into consideration, antibiotic active substances which can be used successfully in dogs with chronic otitis extern were determined as Penicillin G, Amoxicillin Clavulanic Acid, Gentamicin and Ampicillin Sulbactam, respectively. As a result of our study, it was concluded that the causative agent of chronic otitis externa in dogs was detected at the species level and that the investigation of antibiotic susceptibility was beneficial for treatment effectiveness and prevention of resistance.

References

  • Bauer AW, Kirby WM, Sherris JC, Turek M (1966). An� bio� c suscep� bility tes� ng by a standardized single disc method. American Journal of Clinical Pathology, 45(4), 493-496.
  • Bajva J (2019). Canine o� � s externa - Treatment and complica� ons. Canadian Veterinary Journal, 60(1), 97-99.
  • Bilgehan H (1995). Klinik Mikrobiyolojik Tanı. Fakülteler Kitapevi, 2. Basım, İzmir.
  • Blue JL, Wooley RE (1977). An� microbial sensi� vity pa� erns of bacteria isolated from dogs with o� � s externa. Journal of American Veterinary Medical Associa� on, 171, 362-363.
  • Borum AE, Çeçen G, Demir G, Ce� n C, Şentürk S (2014). Köpeklerde O� � s Externa Vakalarından İzole edilen Mikroorganizmalar ve An� bakteriyel Duyarlılıklarının Belirlenmesi. Kocatepe Veterinary Journal, 7(1), 27-31.
  • Bourély C, Cazeau G, Jarrige N, Leblond A, Madec JY, Haenni M, Gay E (2019). An� microbial resistance pa� erns of bacteria isolated from dogs with o� � s. Epidemiology and Infec� on, 147, e121, 1–10.
  • CLSI (2002). Performance Standards for An� microbial Disk and Dilu� on Suscep� bility Tests for Bacteria Isolated from Animals. Approved Standard, CLSI Document M31-A2. Second ed. Clinical and Laboratory Standards Ins� tute. Wayne, PA.
  • Cole LK, Kwochka KW, Kowalski JJ, Hillier A (1998). Microbial fl ora and an� microbial suscep� bility pa� erns of isolated pathogens from the horizontal ear canal and middle ear in dogs with o� � s media. Journal of American Veterinary Medical Associa� on, 212, 534-538.
  • Hariharan H, Coles M, Poole D, Lund L, Page R (2006). Update on an� microbial suscep� bility of bacterial isolates from canine and feline o� � s externa. Canadian Veterinary Journal, 47, 253-255.
  • Harvey RG, Harari J, Delauche AJ (2001). Ear Diseases of the Dog and Cat. Manson Publishing, London.
  • Huang HP, Li� le CJL, McNeil PE (2009). Histological changes in the external ear canal of dogs with o� � s externa. Veterinary Dermatology, 20, 422-428.
  • Keskin O, Kökçü L, Akan M (1999). O� � s eksternalı köpeklerden izole edilen mikroorganizmalar ve an� biyo� k duyarlılıkları. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 46, 163-168.
  • Kiss G, Radvanyi S, Szige� G (1997). New combina� on for the therapy of canine o� � s externa. I. Microbiology of o� � s externa. Journal of Small Animal Prac� ce, 38, 51-56.
  • Koneman E, Allen DS, Jonda WM, Schreckenberger PC, Winn WCJ (1997). Color Atlas and Textbook of Diagnos� c Microbiology. 5th Edith., Lippinco� Press, Philadelphia, pp. 268.
  • Kuyucuoğlu Y, Sarıtaş ZK (2010). Sağlıklı Köpeklerin Dış Kulak Kanalından İzole Edilen Mikroorganizmalar ve An� biyo� k Duyarlılıkları. Kocatepe Veteriner Dergisi, 3(2), 19-23.
  • Lilenbaum W, Aquino MHC, Costa MNA, Souza Ne� o BA, Souza ET (1987). Bacterial o� � s in dogs. Medical Science, 6, 47-52.
  • Lilenbaum W, Veras M, Blum E, Souza GN (2000). An� microbial suscep� bility of Staphylococci isolated from o� � s externa in dogs. Le� ers in Applied Microbiology, 31, 42-45.
  • Lyskova, P, Vydrzalova, M and Mazurova, J (2007). Iden� Ş ca� on and an� microbial suscep� bility of bacteria and yeasts isolated from healthy dogs and dogs with o� � s externa. Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine, 54, 559-563.
  • Malayeri HZ, Jamshidi S, Salehi TZ (2010). Iden� Ş ca� on and an� microbial suscep� bility pa� erns of bacteria causing o� � s externa in dogs. Veterinary Research Communica� ons, 34, 435-444.
  • Mar� n B, Lubida G, Gonzales L, Tejedor J (2001). An� bacterial Suscep� bility Pa� erns of Pseudomonas Strains Isolated from Chronic Canine O� � s Externa. Jama Veterinary Medical B Infec� ons Public Healty, 47(3), 191-196.
  • May ER, Hnilica KA, Frank LA, Jones RD, Bemis DA (2005). Isola� on of Staphylococcus schleiferi from healthy dogs and dogs with o� � s, pyoderma, or both. Journal of the American Veterinary Medical Associa� on, 227, 928-931.
  • Petrov V, Mihaylov G, Tsachev I, Zhelev G, MArutsov P, Koev K (2013). O� � s externa in dogs: microbiology and an� microbial suscep� bility. Revue de Médecine Vétérinaire, 164(1), 18-22.
  • Rosser EJ (2004). Causes of o� � s externa. Veterinary Clinics: Small Animal Prac� ce, 34, 459-468.
  • Rosser EJ (2004). Causes of o� � s externa. Veterinary Clinics: Small Animal Prac� ce, 34, 459-468.
  • Saridomichelakis MN, Farmaki R, Leon� des LS, Kou� nas AF (2007). Ae� ology of canine o� � s externa: a retrospec� ve study of 100 cases. Veterinary Dermatology, 18(5), 341-347.
  • Sarıerler M, Kırkan Ş (2004). Microbiological Diagnosis and Therapy of Canine O� � s Externa. Veteriner Cerrahi Dergisi,10 (3-4), 11-15.
  • Schick AE, Angus JC, Coyner KS (2007). Variability of laboratory iden� Ş ca� on and an� bio� c suscep� bility repor� ng of Pseudomonas spp. isolates from dogs with chronic o� � s externa. Veterinary Dermatology, 18(2), 120-126.
  • Silva N (2001). Iden� Ş ca� on and an� microbial suscep� bility pa� erns of Staphylococcus spp. Isolated from canine chronic o� � s externa. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, 53(2), 1-5.
  • Tejedor JMT, Mar� n BJL. Iden� Ş ca� on and an� microbial suscep� bility of coagulase posi� ve staphylococci isolated from healthy dogs and dogs suff ering from o� � s externa. Journal of Veterinary Medicine Series B 2002;49(9):419-423.

Köpeklerde Kronik Otitis Eksterna’ya Sebep Olan Mikroorganizmaların Dağılımı ve Antibiyotiklere Duyarlılıklarının Belirlenmesi

Year 2019, Volume: 8 Issue: 1, 638 - 641, 01.01.2019

Abstract

Özbilgi/Amaç: Otitis eksterna köpeklerde görülen en yaygın kulak hastalıklarının başında gelmektedir. Özellikle kronik otitis eksternalı köpeklerde çeşitli patojen mikroorganizmalara sıklıkla rastlanılmaktadır. Bu çalışmada, kronik otitis eksternalı köpeklerden izole edilen mikroorganizma türlerinin araştırılması ve antibiyotik duyarlılıklarının belirlenmesi amaçlanmıştır. Materyal ve metot: Araştırmada kronik otitis eksternalı köpeklerden alınan 22 adet svap örneği kullanılmıştır. Örnekler %7 Koyun Kanlı agar, MacConkey agar ve Saboraud Dextrose agarlara ekilmiş ve üreyen mikroorganizmalar fenotipik yöntemler ile izole edilmiştir. İzolatların antibiyotik duyarlılık testleri ise disk difüzyon yöntemi kullanılarak test edilmiştir. Sonuç: Örneklerin 22’sinde de mikroorganizma üremeleri gözlemlenmiştir. Bunlardan %64’ünde tekli, %36’sında ise çoklu üremeler olmuştur. Çalışmada, en çok Koagulaz Negatif Staphylococcus sp. KNS %59 , bunu sırasıyla Pseudomonas sp. %18 , Staphylococcus aureus %9 , Corynebacterium sp. %9 ve Streptococcus sp. %5 izole edilmiştir. Fungal etkenlerden ise, üreme görülen 22 örneğin %14’ünde Candida sp., %9’unda Aspergillus sp., %5’inde ise Trichophyton sp. üremesi belirlenmiştir. Bakterilerin antibiyotik duyarlılıklarının belirlenmesinde 8 farklı etken madde kullanılmıştır. Tüm bakteriler göz önüne alındığında kronik otitis eksternalı köpeklerde başarı ile kullanılabilecek antibiyotik etken maddeleri sırasıyla Penisilin G, Amoksisilin Klavulanik Asit, Gentamisin ve Ampisilin Sulbaktam olarak tespit edilmiştir. Çalışmamız sonucunda, köpeklerde kronik otitis eksternaya sebep olan etkenin tür düzeyinde saptanıp antibiyotik duyarlılığının araştırılmasının tedavinin etkinliği ve direnç gelişiminin önlenmesi açısından yararlı olacağı kanısına varılmıştır.

References

  • Bauer AW, Kirby WM, Sherris JC, Turek M (1966). An� bio� c suscep� bility tes� ng by a standardized single disc method. American Journal of Clinical Pathology, 45(4), 493-496.
  • Bajva J (2019). Canine o� � s externa - Treatment and complica� ons. Canadian Veterinary Journal, 60(1), 97-99.
  • Bilgehan H (1995). Klinik Mikrobiyolojik Tanı. Fakülteler Kitapevi, 2. Basım, İzmir.
  • Blue JL, Wooley RE (1977). An� microbial sensi� vity pa� erns of bacteria isolated from dogs with o� � s externa. Journal of American Veterinary Medical Associa� on, 171, 362-363.
  • Borum AE, Çeçen G, Demir G, Ce� n C, Şentürk S (2014). Köpeklerde O� � s Externa Vakalarından İzole edilen Mikroorganizmalar ve An� bakteriyel Duyarlılıklarının Belirlenmesi. Kocatepe Veterinary Journal, 7(1), 27-31.
  • Bourély C, Cazeau G, Jarrige N, Leblond A, Madec JY, Haenni M, Gay E (2019). An� microbial resistance pa� erns of bacteria isolated from dogs with o� � s. Epidemiology and Infec� on, 147, e121, 1–10.
  • CLSI (2002). Performance Standards for An� microbial Disk and Dilu� on Suscep� bility Tests for Bacteria Isolated from Animals. Approved Standard, CLSI Document M31-A2. Second ed. Clinical and Laboratory Standards Ins� tute. Wayne, PA.
  • Cole LK, Kwochka KW, Kowalski JJ, Hillier A (1998). Microbial fl ora and an� microbial suscep� bility pa� erns of isolated pathogens from the horizontal ear canal and middle ear in dogs with o� � s media. Journal of American Veterinary Medical Associa� on, 212, 534-538.
  • Hariharan H, Coles M, Poole D, Lund L, Page R (2006). Update on an� microbial suscep� bility of bacterial isolates from canine and feline o� � s externa. Canadian Veterinary Journal, 47, 253-255.
  • Harvey RG, Harari J, Delauche AJ (2001). Ear Diseases of the Dog and Cat. Manson Publishing, London.
  • Huang HP, Li� le CJL, McNeil PE (2009). Histological changes in the external ear canal of dogs with o� � s externa. Veterinary Dermatology, 20, 422-428.
  • Keskin O, Kökçü L, Akan M (1999). O� � s eksternalı köpeklerden izole edilen mikroorganizmalar ve an� biyo� k duyarlılıkları. Ankara Üniversitesi Veteriner Fakültesi Dergisi, 46, 163-168.
  • Kiss G, Radvanyi S, Szige� G (1997). New combina� on for the therapy of canine o� � s externa. I. Microbiology of o� � s externa. Journal of Small Animal Prac� ce, 38, 51-56.
  • Koneman E, Allen DS, Jonda WM, Schreckenberger PC, Winn WCJ (1997). Color Atlas and Textbook of Diagnos� c Microbiology. 5th Edith., Lippinco� Press, Philadelphia, pp. 268.
  • Kuyucuoğlu Y, Sarıtaş ZK (2010). Sağlıklı Köpeklerin Dış Kulak Kanalından İzole Edilen Mikroorganizmalar ve An� biyo� k Duyarlılıkları. Kocatepe Veteriner Dergisi, 3(2), 19-23.
  • Lilenbaum W, Aquino MHC, Costa MNA, Souza Ne� o BA, Souza ET (1987). Bacterial o� � s in dogs. Medical Science, 6, 47-52.
  • Lilenbaum W, Veras M, Blum E, Souza GN (2000). An� microbial suscep� bility of Staphylococci isolated from o� � s externa in dogs. Le� ers in Applied Microbiology, 31, 42-45.
  • Lyskova, P, Vydrzalova, M and Mazurova, J (2007). Iden� Ş ca� on and an� microbial suscep� bility of bacteria and yeasts isolated from healthy dogs and dogs with o� � s externa. Journal of Veterinary Medicine. A, Physiology, Pathology, Clinical Medicine, 54, 559-563.
  • Malayeri HZ, Jamshidi S, Salehi TZ (2010). Iden� Ş ca� on and an� microbial suscep� bility pa� erns of bacteria causing o� � s externa in dogs. Veterinary Research Communica� ons, 34, 435-444.
  • Mar� n B, Lubida G, Gonzales L, Tejedor J (2001). An� bacterial Suscep� bility Pa� erns of Pseudomonas Strains Isolated from Chronic Canine O� � s Externa. Jama Veterinary Medical B Infec� ons Public Healty, 47(3), 191-196.
  • May ER, Hnilica KA, Frank LA, Jones RD, Bemis DA (2005). Isola� on of Staphylococcus schleiferi from healthy dogs and dogs with o� � s, pyoderma, or both. Journal of the American Veterinary Medical Associa� on, 227, 928-931.
  • Petrov V, Mihaylov G, Tsachev I, Zhelev G, MArutsov P, Koev K (2013). O� � s externa in dogs: microbiology and an� microbial suscep� bility. Revue de Médecine Vétérinaire, 164(1), 18-22.
  • Rosser EJ (2004). Causes of o� � s externa. Veterinary Clinics: Small Animal Prac� ce, 34, 459-468.
  • Rosser EJ (2004). Causes of o� � s externa. Veterinary Clinics: Small Animal Prac� ce, 34, 459-468.
  • Saridomichelakis MN, Farmaki R, Leon� des LS, Kou� nas AF (2007). Ae� ology of canine o� � s externa: a retrospec� ve study of 100 cases. Veterinary Dermatology, 18(5), 341-347.
  • Sarıerler M, Kırkan Ş (2004). Microbiological Diagnosis and Therapy of Canine O� � s Externa. Veteriner Cerrahi Dergisi,10 (3-4), 11-15.
  • Schick AE, Angus JC, Coyner KS (2007). Variability of laboratory iden� Ş ca� on and an� bio� c suscep� bility repor� ng of Pseudomonas spp. isolates from dogs with chronic o� � s externa. Veterinary Dermatology, 18(2), 120-126.
  • Silva N (2001). Iden� Ş ca� on and an� microbial suscep� bility pa� erns of Staphylococcus spp. Isolated from canine chronic o� � s externa. Arquivo Brasileiro de Medicina Veterinaria e Zootecnia, 53(2), 1-5.
  • Tejedor JMT, Mar� n BJL. Iden� Ş ca� on and an� microbial suscep� bility of coagulase posi� ve staphylococci isolated from healthy dogs and dogs suff ering from o� � s externa. Journal of Veterinary Medicine Series B 2002;49(9):419-423.
There are 29 citations in total.

Details

Primary Language English
Journal Section Research Article
Authors

Göksel Erbaş This is me

Şükrü Kırkan This is me

Uğur Parın This is me

H. Tuğba Yüksel This is me

Publication Date January 1, 2019
Published in Issue Year 2019 Volume: 8 Issue: 1

Cite

APA Erbaş, G., Kırkan, Ş., Parın, U., Yüksel, H. T. (2019). Distribution of Microorganisms That Cause Otitis Externa in Dogs and Determination of Antibiotic Susceptibilities. Animal Health Production and Hygiene, 8(1), 638-641.