Yerli Kara sığır ırkı ve Anadolu mandalarında SMO gen polimorfizmi

Yıl 2021, , 29 - 32, 30.06.2021

Mervan Bayraktar , İbrahim Aytekin

https://doi.org/10.25308/aduziraat.786132

Öz

Bu çalışmanın amacı hem Yerli Kara sığırlarında hem de Anadolu mandalarında PCR-RFLP methodu ile Cfr13I restriksiyon enzimi kullanılarak Bovine Smoothened (SMO) gen polimorfizminin 9. ekzonundaki (G21234C) G>C mutasyonunu belirlemektir. SMO geninin 9. ekzonundaki G>C mutasyonuna göre sonuçlar Yerli Kara sığırlarda GG, GC ve CC genotiplerinin olduğunu, fakat Anadolu mandalarında yalnızca GG genotipinin olduğunu göstermiştir. Yerli Kara sığırlarda allel ve genotip frekansları sırasıyla G ve C allelleri için 0.35 ve 0.65, GG, GC ve CC genotipleri için 0.22, 026 ve 0.52 olarak tahmin edilmiştir. Ki-kare testi Yerli Kara sığır populasyonunun G21234C değişikliği ile ilgili olarak Hardy-Weinberg dengesinde olmadığını göstermiştir (P <0.05).
SMO geninin 9. ekzon (G21234C) üzerindeki G> C mutasyonu sığırlarda polimorfik olduğu için SMO geninin potansiyel bir aday gen olduğu sonucuna varılabilir. Fakat, Anadolu mandalarında monomorfik olduğu için mandalar için böyle bir ifade söylenemez.

Anahtar Kelimeler

Bovine, Smoothened, çiftlik hayvanları, Cfr13I, restriksiyon enzimi

Bovine SMO gene polymorphism in Anatolian Black Cattle and Anatolian Water Buffaloes

Öz

The purpose of this study was to determine the G>C mutation on exon 9 (G21234C) of Bovine Smoothened (SMO) gene polymorphism by using Cfr13I restriction enzyme with PCR-RFLP method in both Anatolian Black cattle and Anatolian Water Buffaloes. According to the G>C mutation on exon 9 (G21234C) of SMO gene, the results showed that there were three genotypes such as GG, GC and CC in Anatolian Black cattle, but there was only one genotype GG in Anatolian Water Buffaloes. Allele and genotype frequencies in Anatolian Black cattle were estimated as 0.35 and 0.65 for G and C alleles and 0.22, 026 and 0.52 for GG, GC and CC genotypes, respectively. The Chi-square test showed that the Anatolian Black cattle population was not in Hardy-Weinberg equilibrium with respect to G21234C substitution (P<0.05).
Because G>C mutation on exon 9 (G21234C) of SMO gene is polymorphic in cattle, it can be concluded that SMO gene is a potential candidate gene. However, such a statement cannot be expressed for buffaloes since it is monomorphic in Anatolian buffaloes.

Anahtar Kelimeler

Bovine, Smoothened, livestock, Cfr13I, restriction enzyme

Kaynakça

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