Zencefilin (Zingiber officinale Roscoe) Diazinon Toksisitesine Karşı Koruyucu Rolünün Allium cepa L. Meristem Hücrelerinde Araştırılması

Yıl 2021, , 65 - 72, 30.06.2021

Kültiğin Çavuşoğlu , Oksal Macar , Tuğçe Kalefetoğlu Macar , Emine Yalçın

https://doi.org/10.25308/aduziraat.814545

Öz

Yaygın olarak kullanılan bir pestisit olan diazinon hedef olmayan canlılar üzerinde toksik etkilere sahiptir. Bu çalışma, diazinonun meristem hücrelerinde teşvik ettiği toksisiteye karşı zencefilin koruyucu ve iyileştirici etkisini fizyolojik, genotoksik ve biyokimyasal parametrelerle araştırmayı hedeflemiştir. Ayrıca kök meristem hücrelerinde meydana gelen hasarlar incelenmiştir. Bu amaçla A. cepa soğanları 6 gruba ayrıldı (kontrol, 240 mg/L zencefil, 480 mg/L zencefil, 500 mg/L diazinon, 500 mg/L diazinon + 240 mg/L zencefil ve 500 mg/L diazinon + 480 mg/L zencefil). Diazinon süperoksit dismutaz aktivitesi, katalaz aktivitesi, malondialdehit seviyesi, mikronükleus sıklığı, kromozomal anormallik yoğunluğu ve meristematik hücre hasarlarını arttırmış; ancak mitotik indeksi, çimlenme yüzdesi, kök uzunluğu, ağırlık artışı azaltmıştır. Zencefil, diazinon ile birlikte uygulandığında doza bağlı olarak tüm bu toksisite göstergelerini düşürmüştür. Bu çalışma, A. cepa modeli kullanılarak diazinonun hedef olmayan organizmalar üzerindeki toksik etkilerine karşı zencefilin koruyucu bir rolünü açıkça göstermiştir.

Anahtar Kelimeler

antioksidant, fonksiyonel gıda, genotoksisite, mikronükleus

Destekleyen Kurum

destek alınmamıştır

Investigation of the Protective Role of Ginger (Zingiber officinale Roscoe) Against Diazinone Toxicity in Allium cepa L. Meristem Cells

Öz

Diazinon, a widely used pesticide, has toxic effects on non-target organisms. This study aimed to investigate the protective and healing effect of ginger against the toxicity promoted by diazinon in meristem cells with physiological, genotoxic and biochemical parameters. In addition, root meristem cell damages were examined. For this purpose, A. cepa bulbs were divided into 6 groups (control, 240 mg/L ginger, 480 mg/L ginger, 500 mg/L diazinone, 500 mg/L diazinone + 240 mg/L ginger and 500 mg/L diazinone + 480 mg/L ginger). Diazinon increased superoxide dismutase activity, catalase activity, malondialdehyde level, micronucleus frequency, chromosomal abnormality density and meristematic cell damage; but reduced the mitotic index, germination percentage, root length, weight gain. Ginger reduced all these toxicity indicators depending on the dose when administered with diazinon. This study clearly demonstrated the protective role of ginger against the toxic effects of diazinon on non-target organisms using the A. cepa model.

Anahtar Kelimeler

antioxidant, functional food, genotoxicity, micronucleus

Kaynakça

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