A Survey of Raw Milk For Microbiological Quality and Typing of Foodborne Pathogens by MALDI-TOF MS

Year 2019, Volume: 16 Issue: 2, 185 - 191, 31.12.2019

Tuğba Cebeci

https://doi.org/10.25308/aduziraat.575681

Cited By: 2

Abstract

This study was conducted in
order to determine microbiological quality of raw cow milk samples sold in
public markets and to investigate foodborne pathogens in Giresun. The samples
were examined for  total mesophilic
aerobic bacteria, total  psychrotroph aerobic bacteria, coliform, Enterobacteriaceae, Micrococcus/Staphylococcus, lactic acid bacteria, yeast and mold, Salmonella spp., Escherichia coli O157:H7, L.
monocytogenes, Bacillus cereus, Campylobacter spp. according to Food and
Drug Administration/Bacteriological Analytical Manual and International
Organization for Standardization methods. Identification of the isolated
pathogens was made with Matrix-assisted laser desorption,  ionization time of flight mass spectrometry.
The mean values of microorganism colonies detected in raw cow milk samples were
as follows: total mesophilic aerobic bacteria count 5.87 log cfu/ml in 93
samples; total  psychrotroph aerobic bacteria count 5.69 log cfu/ml in 95
samples; coliform count 4.85 log cfu/ml in 12 samples; Enterobacteriaceae count 4.84 log cfu/ml in 18 samples; Micrococcus/Staphylococcus count 5.17 log cfu/ml in 94 samples; lactic acid
bacteria count 5.54 log cfu/ml in 97 samples, and yeast and mold 5.16 log
cfu/ml in 73 samples. Listeria
monocytogenes, Escherichia coli were
found as 6 (6%) and 34 (34%) in 100 raw cow milk samples respectively. Salmonella spp., Campylobacter spp., Escherichia
coli O157:H7 and Bacillus cereus were
not detected. As a result; a statistically significant presence of bacteria
threatening human health was detected in the raw cow milk samples examined. In
order to resolve this problem, it is thought that further similar studies
should be conducted on the basis of procuders, consumers and supervisory
institutions.

Keywords

milk, microbiological quality, food pathogens, MALDI-TOF MS

Supporting Institution

Giresun Üniversitesi

Project Number

FEN-BAP-A-170417-114

Çiğ Süt Örneklerinin Mikrobiyolojik Kalitesi ve Gıda Patojenlerinin MALTI-TOF MS ile Tiplendirilmesi Üzerine Bir Araştırma

Abstract

Bu çalışma, Giresun'da pazarlarda satılan çiğ inek
sütü örneklerinin mikrobiyolojik kalitesini belirlemek ve gıda patojenlerini
araştırmak amacıyla gerçekleştirilmiştir. Örnekler, toplam mezofilik aerobik
bakteri, toplam psikrotrof aerobik bakteri, koliform grubu bakteri,
Enterobacteriaceae, Micrococcus/Staphylococcus, laktik asit bakterileri, maya
ve küf, Salmonella spp., Escherichia coli O157:H7, Listeria monocytogenes,
Bacillus cereus, Campylobacter spp. yönünden Food and Drug Administration/Bacteriological
Analytical Manual ve International Organization for Standardization
yöntemlerine göre incelenmiştir. İzole edilen patojenlerin identifikasyonları,
Matriks aracılı lazer dezorpsiyon, iyonizasyon uçuş zamanı kütle spektrometresi
ile yapılmıştır. Çiğ inek sütü örneklerinde belirlenen mikroorganizma
kolonilerinin ortalama değerleri sırasıyla; toplam mezofilik aerobik bakteri
sayısı 93 örnekte 5.87 log kob/ml; toplam psikrotrof aerobik bakteri sayısı 95
örnekte 5.69 log kob/ml; koliform sayısı 12 örnekte 4.85 log kob/ml;
Enterobacteriaceae sayısı 18 örnekte 4.84 log kob/ml;
Micrococcus/Staphylococcus sayısı 94 örnekte 5.17 log kob/ml; laktik asit
bakteri sayısı, 97 örnekte 5.54 log kob/ml ve maya-küf sayısı 73 örnekte 5.16
log kob/ml olarak bulunmuştur. Listeria monocytogenes ve Escherichia coli, 100
çiğ inek sütü örneğinde sırasıyla 6 (%6) ve 34 (%34) olarak bulunmuştur.
Salmonella spp., Campylobacter spp., Escherichia coli O157: H7 ve Bacillus
cereus saptanmamıştır. Sonuç olarak; araştırmada incelenen çiğ inek sütü
örneklerinde, istatistiki olarak önemli düzeyde, insan sağlığını tehdit eden
bakterilerin varlığı tespit edilmiştir. Bu durumun düzeltilmesi için, üretici,
tüketici ve denetleyici kurumlar bazında benzer daha çok sayıda çalışmaların
yapılarak kamuoyuna duyurulması gerektiği düşünülmektedir.

Keywords

süt, mikrobiyolojik kalite, gıda patojenleri, MALDI-TOF MS

Project Number

FEN-BAP-A-170417-114

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