Yerli Kara sığır ırkı ve Anadolu mandalarında SMO gen polimorfizmi

Year 2021, Volume: 18 Issue: 1, 29 - 32, 30.06.2021

Mervan Bayraktar İbrahim Aytekin

https://doi.org/10.25308/aduziraat.786132

Abstract

Bu çalışmanın amacı hem Yerli Kara sığırlarında hem de Anadolu mandalarında PCR-RFLP methodu ile Cfr13I restriksiyon enzimi kullanılarak Bovine Smoothened (SMO) gen polimorfizminin 9. ekzonundaki (G21234C) G>C mutasyonunu belirlemektir. SMO geninin 9. ekzonundaki G>C mutasyonuna göre sonuçlar Yerli Kara sığırlarda GG, GC ve CC genotiplerinin olduğunu, fakat Anadolu mandalarında yalnızca GG genotipinin olduğunu göstermiştir. Yerli Kara sığırlarda allel ve genotip frekansları sırasıyla G ve C allelleri için 0.35 ve 0.65, GG, GC ve CC genotipleri için 0.22, 026 ve 0.52 olarak tahmin edilmiştir. Ki-kare testi Yerli Kara sığır populasyonunun G21234C değişikliği ile ilgili olarak Hardy-Weinberg dengesinde olmadığını göstermiştir (P <0.05).
SMO geninin 9. ekzon (G21234C) üzerindeki G> C mutasyonu sığırlarda polimorfik olduğu için SMO geninin potansiyel bir aday gen olduğu sonucuna varılabilir. Fakat, Anadolu mandalarında monomorfik olduğu için mandalar için böyle bir ifade söylenemez.

Keywords

Bovine, Smoothened, çiftlik hayvanları, Cfr13I, restriksiyon enzimi

Bovine SMO gene polymorphism in Anatolian Black Cattle and Anatolian Water Buffaloes

Abstract

The purpose of this study was to determine the G>C mutation on exon 9 (G21234C) of Bovine Smoothened (SMO) gene polymorphism by using Cfr13I restriction enzyme with PCR-RFLP method in both Anatolian Black cattle and Anatolian Water Buffaloes. According to the G>C mutation on exon 9 (G21234C) of SMO gene, the results showed that there were three genotypes such as GG, GC and CC in Anatolian Black cattle, but there was only one genotype GG in Anatolian Water Buffaloes. Allele and genotype frequencies in Anatolian Black cattle were estimated as 0.35 and 0.65 for G and C alleles and 0.22, 026 and 0.52 for GG, GC and CC genotypes, respectively. The Chi-square test showed that the Anatolian Black cattle population was not in Hardy-Weinberg equilibrium with respect to G21234C substitution (P<0.05).
Because G>C mutation on exon 9 (G21234C) of SMO gene is polymorphic in cattle, it can be concluded that SMO gene is a potential candidate gene. However, such a statement cannot be expressed for buffaloes since it is monomorphic in Anatolian buffaloes.

Keywords

Bovine, Smoothened, livestock, Cfr13I, restriction enzyme

References

• Alcedo J, Ayzenzon M, Von Ohlen T, Noll M, Hooper JE (1996) The Drosophila smoothened gene encodes a seven-pass membrane protein, a putative receptor for the hedgehog signal. Cell 86: 221-232.
• Avci O (2012) Hedgehog/Hedgehog. Turk Dermatoloji Dergisi 6: 162.
• Aytekin I, Ozdil F, Zulkadir U, Boztepe S, Sariyel V (2011) Evaluation of ISSR markers for genetic diversity analysis in Anatolian water buffaloes. Journal of the Science of Food and Agriculture 91: 1957-1962.
• Casas E, Kehrli Jr ME (2016) A review of selected genes with known effects on performance and health of cattle. Frontiers in veterinary science 3: 113.
• Fadhil M, Zülkadir U (2017) Molecular Characterization of MSTN Gene in Holstein Friesians and Brown Swiss Cattle Breeds. Selcuk Journal of Agriculture and Food Sciences 31: 151-153.
• Ingham PW, McMahon AP (2001) Hedgehog signaling in animal development: paradigms and principles. Genes & development 15: 3059-3087.
• Ingham PW, Nakano Y, Seger C (2011) Mechanisms and functions of Hedgehog signalling across the metazoa. Nature Reviews Genetics 12: 393-406.
• James AW (2013) Review of signaling pathways governing MSC osteogenic and adipogenic differentiation. Scientifica 2013.
• Jia Y, Wang Y, Xie J (2015) The Hedgehog pathway: role in cell differentiation, polarity and proliferation. Archives of toxicology 89: 179-191.
• Kim J, Aftab BT, Tang JY, Kim D, Lee AH, Rezaee M, Kim J, Chen B, King EM, Borodovsky A (2013) Itraconazole and arsenic trioxide inhibit Hedgehog pathway activation and tumor growth associated with acquired resistance to smoothened antagonists. Cancer cell 23: 23-34.
• Liang S, Chen RT, Zhang DP, Xin HH, Lu Y, Wang MX, Miao YG (2015) Hedgehog signaling pathway regulated the target genes for adipogenesis in silkworm Bombyx mori. Insect science 22: 587-596.
• Murone M, Rosenthal A, de Sauvage FJ (1999) Sonic hedgehog signaling by the patched–smoothened receptor complex. Current Biology 9: 76-84.
• Quijada L, Callejo A, Torroja C, Guerrero I (2007) The patched receptor: Switching on/off the Hedgehog signaling pathway. Hedgehog-Gli Signaling in Human Disease: 23.
• Ruiz-Gómez A, Molnar C, Holguín H, Mayor Jr F, de Celis JF (2007) The cell biology of Smo signalling and its relationships with GPCRs. Biochimica et Biophysica Acta (BBA)-Biomembranes 1768: 901-912.
• Soysal MI, Tekerli M, Daşkıran İ (2013) Anatolian water buffaloes husbandry in Turkey. Buffalo Bulletin 32: 293-309.
• Steg AD, Katre AA, Bevis KS, Ziebarth A, Dobbin ZC, Shah MM, Alvarez RD, Landen CN (2012) Smoothened antagonists reverse taxane resistance in ovarian cancer. Molecular cancer therapeutics 11: 1587-1597.
• Wu X, Walker J, Zhang J, Ding S, Schultz PG (2004) Purmorphamine induces osteogenesis by activation of the hedgehog signaling pathway. Chemistry & biology 11: 1229-1238.
• Yilmaz O, Akin O, Yener SM, Ertugrul M, Wilson R (2012) The domestic livestock resources of Turkey: cattle local breeds and types and their conservation status. Animal Genetic Resources 50: 65-74.
• Zhang Y-R, Gui L-S, Li Y-K, Jiang B-J, Wang H-C, Zhang Y-Y, Zan L-S (2015) Molecular characterization of bovine SMO gene and effects of its genetic variations on body size traits in Qinchuan cattle (Bos taurus). International journal of molecular sciences 16: 16966-16980.
• Zhang Y, Li Y, Fu C, Wang J, Wang H, Zan L (2014) Effects of bovine SMO gene polymorphisms on the body measurement and meat quality traits of Qinchuan cattle. Genet. Mol. Res 13: 8105-8117.
• Zimin AV, Delcher AL, Florea L, Kelley DR, Schatz MC, Puiu D, Hanrahan F, Pertea G, Van Tassell CP, Sonstegard TS (2009) A whole-genome assembly of the domestic cow, Bos taurus. Genome biology 10: R42.