Phytoseiulus persimilis Athias-Henriot (Acari: Phytoseiidae)'de Pirimicarb Seleksiyonu ile Elde Edilen Direnç, Asetilkolinesteraz (AChE) Enzim Aktiviteleri ve Gen Bölgesinin Kısmi Dizilimi

Yıl 2023, Cilt: 20 Sayı: 1, 55 - 62, 30.06.2023

Gizem Berber , Sibel Yorulmaz

https://doi.org/10.25308/aduziraat.1189053

Öz

Tarım alanlarında zararlı tetranychid türlerin mücadelesinde öncelikli olarak kimyasal mücadele kullanılmaktadır. Bu zararlılar ile etkin bir şekilde beslenen avcı akar popülasyonlarıda pestisitlerden etkilenmektedir. Bu çalışmada, Phytoseiulus persimilis Athias-Henriot (Acari: Phytoseiidae) üzerinde laboratuar koşullarında pirimicarb seleksiyon baskısı sonucu direnç, asetilkolinesteraz (AChE) enzimi ve mutasyonları arasındaki ilişkilerin belirlenmesi amaçlanmıştır. Çalışma, yaprak disk metoduyla ilaçlama kulesi kullanılarak yapılmıştır. Yedi seleksiyon sonucunda avcı akarda belirlenen pirimicarb direnci 45.5 kata kadar artmıştır. IBP ve TPP sinerjistlerinin pirimicarb üzerinde herhangi bir etkisi belirlenememiştir. Başlangıç ve pirimicarb dirençli P. persimilis popülasyonlarında bakılan hedef bölge mutasyonları (F331W ve G119S) belirlenememiştir, ancak ileriki çalışmalarda farklı hedef bölge mutasyonlarının da incelenmesinin faydalı olacağı düşünülmektedir. Çalışma, P. persimilis'de pirimicarb direnci ve AChE nokta mutasyonları üzerinde yapılan ilk araştırma olması nedeniyle önem taşımaktadır.

Anahtar Kelimeler

: Phytoseiulus persimilis, pirimicarb, AchE, mutasyon

Destekleyen Kurum

Isparta Uygulamalı Bilimler Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi

Proje Numarası

2020-BTAP2-0081

Teşekkür

Çalışmayı 2020-BTAP2-0081 proje ile maddi olarak destekleyen Isparta Uygulamalı Bilimler Üniversitesi Bilimsel Araştırma Projeleri Koordinasyon Birimi'ne teşekkür ederiz. Ayrıca moleküler çalışmalar sırasında yardımlarını esirgemeyen Dr. Emre İNAK'a teşekkür ederiz.

Resistance Obtained by Pirimicarb Selection, Acetylcholinesterase (AChE) Enzyme Activities and Partial Sequence of the Gene Region in Phytoseiulus persimilis Athias-Henriot (Acari: Phytoseiidae)

Öz

Chemical control is primarily used in the management of tetranychid species agricultural areas.Predatory mite populations that feed on these pests are also affected by the pesticides. In this study, it was aimed to determine the relationships between resistance, acetylcholinesterase (AChE) enzyme and mutations as a result of pirimicarb selection pressure on Phytoseiulus persimilisAthias-Henriot (Acari: Phytoseiidae) under laboratory conditions. The study was carried out using the spray tower with the leaf disc method. As a result of seven selections, pirimicarb resistance increased up to 45.5-fold. No effect of IBP and TPP synergists on pirimicarb weredetermined. The target site mutations (F331W and G119S) examined in the initial and pirimicarb resistant P. persimilis populations were also not determined, but it is thought that it would be useful to examine different target site mutations in future studies. The study, is the first to investigate pirimicarb resistance and AChE point mutations in P. persimilis.

Anahtar Kelimeler

Phytoseiulus persimilis, pirimicarb, AchE, mutation

Proje Numarası

2020-BTAP2-0081

Kaynakça

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