LACCASE PRODUCTION BY NEWLY ISOLATED GANODERMA LUCIDUM WITH SOLID STATE FERMENTATION CONDITIONS AND ITS USING FOR DYE DECOLORIZATION

Year 2022, Volume: 9 Issue: 17, 458 - 470, 31.08.2022

Filiz Boran Özfer Yeşilada

https://doi.org/10.54365/adyumbd.1107682

Cited By: 1

Abstract

Laccase production capacity of newly isolated fungus Ganoderma lucidum was studied during solid state fermentation on solid substrates. Because wheat bran was detected as the most effective solid substrate, effect of various culture parameters and some inducers on laccase production in wheat bran media was investigated. Wheat bran with moisture content of 75%, pH 5.0 and 30 °C was the most effective medium and 10 mM copper addition supported the highest amount of laccase. In glass tray fermenter 2973±220 U/L laccase activity was obtained. Crude laccase gave the highest activity at 70 °C. It was stable at 60°C for 6 hours and retained 37% activity at 70 °C for 2 hours. It decolorized 61% of Remazol Brilliant Blue R within 20 min. Results showed that this strain could produce high amount of laccase and suitable inducer such as copper could induce its laccase production. The crude laccase obtained could be used for textile dye decolorization applications.

Keywords

Ganoderma lucidum, Laccase, Remazol Brilliant Blue R, Solid state fermentation, Wheat bran

Supporting Institution

INONU UNIVERSITY

Project Number

2010-118

Thanks

We thank to Inonu University Research Fund (Project no: 2010-118) for financial support of this study.

YENİ İZOLE EDİLMİŞ GANODERMA LUCIDUM İLE KATI HAL FERMANTASYONU KOŞULLARINDA LAKKAZ ÜRETİMİ VE BOYA RENK GİDERİMİNDE KULLANILMASI

Abstract

Yeni izole edilmiş fungus Ganoderma lucidum' un lakkaz üretim kapasitesi, katı substratlar üzerinde katı ortam fermantasyonu sırasında incelenmiştir. Buğday kepeğinin en etkili katı substrat olduğu tespit edildiğinden, buğday kepeği ortamında çeşitli kültür parametrelerinin ve bazı indükleyicilerin lakkaz üretimine etkisi araştırılmıştır., pH 5.0, 30 °C ve %75 nem içeriği ile buğday kepeği en etkili ortamdı ve 10 mM bakır ilavesi en yüksek lakkaz miktarını destekledi. Cam tava fermentörde 2973±220 U/L lakkaz aktivitesi elde edildi. Ham lakkaz 70 °C'de en yüksek aktiviteyi vermiştir. 60°C'de 6 saat stabildi ve 70°C'de 2 saat boyunca %37 aktiviteyi korudu. Ham lakkaz, 20 dakika içinde Remazol Parlak Mavi R' nin %61 rengini giderdi. Sonuçlar, bu suşun yüksek miktarda lakkaz üretebileceğini ve bakır gibi uygun indükleyicilerin lakkaz üretimini indükleyebileceğini gösterdi. Elde edilen ham lakkaz, tekstil boyası renk giderme uygulamaları için kullanılabilir.

Keywords

Ganoderma lucidum, Lakkaz, Remazol Parlak Mavi R, Katı ortam fermentasyonu, Buğday kepeği

Project Number

2010-118

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