Trikalsiyum Fosfat Çözebilen Bazı Bakteri ve Mikrofungusların İzolasyonu ve Karakterizasyonu

Year 2013, Volume: 1 Issue: 1, 11 - 20, 01.06.2013

Mehmet Nuri Aydoğan Ömer Faruk Algur Mustafa Özdemir

Abstract

Bu çalışmada; sıcaklık, pH, tuzluluk vb. özellikler bakımından farklılık gösteren habitatlardan çeşitli bitkilere ait kök örnekleri ve rizosfer toprakları toplandı. Toprak dilüsyonları ve kök özütlerinden, bakteri ve mikrofunguslar için önerilen genel besi yerlerine ekim yapıldı. İnkübasyon sonunda 300 bakteri ve 34 mikrofungus türü izole edilerek saflaştırıldı. Hızlı ve etkin bir yöntem olarak önerilen NBRIP-BPB selektif besiyerleri kullanılarak, 24 bakteri ve 6 mikrofungus türünün, trikalsiyum fosfat çözünürlüğünde etkili oldukları kalitatif olarak belirlendi ve bu türlerin tanıları yapıldı

Keywords

İnorganik fosfat, fosfat çözücü mikroorganizmalar, fosfat çözünürlüğü

Isolation and Characterisation of Some Bacteria and Microfungus Solving Tricalcium Phosphate

Abstract

In this study some plants species roots and rhizospher soils were collected from different habitats have different temperature, pH, salinity properties etc. From these samples were prepared root extracts and soil dilutions. After that it was inoculated to general mediums Nutrient agar, Potato dextrose agar prepared for bacteria and microfungi. After the incubation, 300 bacteria species and 34 microfungi species were isolated and purified from these samples. The ability of inorganic phosphate solubulisation bacteria and microfungy were tested using an effective and rapid method NBRIP-BPB selective medium quantitively. 24 bacteria and 6 microfungi species were determined and identified having ability of solubilization of tricalcium phosphate effectively

Keywords

Inorganic phosphate, Phosphate solubilizing microorganisms, phosphate solubilization

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