İnsan Sağlığında Etkili Bir Bitki Ceviz Tohumu Kabuğu (Juglans regia L.): Antioksidan Aktivitesi ve Sıçanlarda Nefroprotektif Etkisi

Year 2024, Volume: 55 Issue: 2, 89 - 104, 29.05.2024

Esra Palabıyık , Handan Uğuz , Hakan Aşkın , Seda Aşkın , Hülya Akıncıoğlu

https://doi.org/10.17097/agricultureatauni.1418238

Abstract

Çalışmada, Posof (Ardahan/Türkiye) cevizlerinin tohum kabuğu (WSC), fitokimyasal bileşenlerinin ve antioksidan kapasitelerinin belirlenmesi için ekstrakte edilmiştir. WSC'nin (E-WSC) etanol ekstraktındaki biyoaktif bileşenlerin asetilkolinesteraz (AChE) ve butirilkolinesteraz (BChE) inhibitörleri üzerindeki etkileri araştırılmıştır. Ayrıca Triton WR-1339 ile indüklenen hiperlipidemik sıçanların böbrek dokularında antioksidan enzim aktivite parametreleri ölçülmüştür. WSC'deki biyoaktif bileşikler GC-MS sistemi ile tanımlanmıştır. WSC’nin antioksidan özellikleri, Fe+3, Cu+2 ve Fe+3-2,4,6-tripiridil-s-triazin (TPTZ) indirgeyici ajan, 1,1-difenil-2-pikrilhidrazil (DPPH) ve 2,2'- azino-bis (3-etilbenzotiyazolin-6-sülfonik asit) (ABTS) temizleme aktiviteleri kullanılarak ölçülmüştür. 30 adet erkek Wistar sıçanı (300 ± 30 g) kullanılarak yapılan bu analizde, rastgele beş gruba ayrılan sıçanlara aşağıdaki gibi uygulama yapılmıştır; K1: Sağlıklı kontrol grubu, K2: E-WSC (150 mg) o.d., K3: E-WSC (300 mg) o.d., K4: Hiperlipidemik grup i.p., K5: Hiperlipidemik grup i.p. + E-WSC (300 mg) o.d. Böbrek dokularında Süperoksit dismutaz (SOD), katalaz (CAT) ve malondialdehit (MDA) analizleri yapılmıştır. Bu sonuçlara göre E-WSC'nin biyoaktif bileşenlerine bağlı olarak önemli antioksidan aktiviteye sahip olduğu, AChE ve BChE enzimleri üzerinde inhibitör etkiye sahip olduğu ve hiperlipidemiye bağlı böbrek hasarını iyileştirerek oksidatif strese karşı koruyucu etkiye sahip olduğu açıkça belirlenmiştir.

Keywords

Antioksidan aktivite, böbrek, oksidatif stress, Triton WR-1339, ceviz tohumu kabuğu

Ethical Statement

Bu çalışma Atatürk Üniversitesi Tıbbi Deneysel Uygulama ve Araştırma Merkezi (ATADEM) etik kurulunun 28.12.2020 tarih ve 236643897-000-EBYS-1 sayılı kararı ile gerçekleştirilmiştir.

Supporting Institution

Atatürk Üniversitesi

Project Number

9157

Thanks

9157 numaralı Araştırma Başlangıç Destek Projesi (ABDEP) ile deneysel çalışmalarımıza destek veren Atatürk Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü'ne (BAP) teşekkür ederiz.

Walnut Seed Coat (Juglans regia L.), a Plant Effective in Human Health: Antioxidant Activity and in Rats Nephroprotective Effect

Abstract

In the study, the seed coat (WSC) of Posof (Ardahan/Türkiye) walnuts was extracted to determine their phytochemical components and antioxidant capacities. The effects of bioactive components in the ethanol extract of WSC (E-WSC) on acetylcholinesterase (AChE) and butyrylcholinesterase (BChE) inhibitors were investigated. Additionally, antioxidant enzyme activity parameters were measured in the kidney tissues of Triton WR-1339-induced hyperlipidemic rats. Bioactive compounds in WSC were identified by GC-MS system. The antioxidant properties of WSC were measured using Fe+3, Cu+2 and Fe+3-2,4,6-tripyridyl-s-triazine (TPTZ) reducing agent, 1,1-diphenyl-2-picrylhydrazyl (DPPH) and 2,2'- azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) scavenging activities. In this analysis, using 30 male Wistar rats (300 ± 30 g) randomly divided into five groups were treated as follows; K1: Healthy control group, K2: E-WSC (150 mg) o.d., K3: E-WSC (300 mg) o.d., K4: Hyperlipidemic group i.p., K5: Hyperlipidemic group i.p. + E-WSC (300 mg) o.d. Superoxide dismutase (SOD), catalase (CAT) and malondialdehyde (MDA) analyzes were performed in kidney tissues. Based on these results, it was clearly determined that E-WSC has significant antioxidant activity due to its bioactive components, has an inhibitory effect on AChE and BChE enzymes, and has a protective effect against oxidative stress by improving hyperlipidemia-related kidney damage.

Keywords

Antioxidant activity, kidney, oxidative stress, Triton WR-1339, walnut seed coat

Ethical Statement

This study was carried out with the decision of the ethics committee of Atatürk University Medical Experimental Application and Research Center (ATADEM) dated 28.12.2020 and numbered 236643897-000-EBYS-1.

Supporting Institution

Ataturk University

Project Number

9157

Thanks

We would like to thank Atatürk University Scientific Research Projects Coordination Unit (BAP) for their support to our experimental studies with the Research Initial Support Project (ABDEP) numbered 9157.

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