Yabani Prometheum sempervivoides (Fıscher Ex M. Bieb.) H. Ohba'nın Yaprak Çeliği Yoluyla Vejetatif Çoğaltılması: Oksin ve Bazı Maddelerin Etkileri

Year 2024, Volume: 55 Issue: 3, 132 - 141, 29.09.2024

Fazilet Parlakova Karagöz , Atilla Dursun , Kadir Yıldırım

https://doi.org/10.17097/agricultureatauni.1430138

Abstract

Doğal olarak yetişen Prometheum sempervivoides (Fisch. ex Bieb.) H. Ohba, taksonuna ait anaç bitkilerden hazırlanan yaprak çeliklerinin köklenmesinde, farklı köklendirme içeriklerinin [kontrol (su), bitki gelişim düzenleyici-köklendirme hormonunun üç farklı dozu (H1: 0.25 g l-1, H2: 0.50 g l-1 ve H3: 1.0 g l-1), tarçın tozu ve Aleo vera jeli] ve iki farklı dikim zamanının uygulanmasının etkilerini saptamak araştırmanın amacını oluşturmaktadır. Canlılık oranı (%), köklü çelik sayısı (NR), kallus oluşturan çelik sayısı (NC), maksimum kök uzunluğu (MRL), köklenme oranı (%) ve köklenme ölçeği (1-5) parametreleri köklenmesi tamamlanan çelikler için yapılmıştır. Sonuçlar istatistiksel olarak değerlendirilmiştir. En olumlu köklenme etkisi, araştırmada kullanılan ticari köklendirme hormonunun en yüksek dozu olan H3 uygulamasında belirlenmiştir. P. sempervivoides yaprak çeliklerinin köklenmesinde toz tarçın uygulamasının Aloe vera jel uygulamasına göre daha etkili bir doğal ekstrakt olduğu belirlenmiştir. Farklı dikim zamanlarının etkilerinin de incelendiği çalışmamız sonunda, P. sempervivoides yaprak çeliklerinin ana bitkiden alınır alınmaz dikilmesinin köklenme üzerine olumlu etkisi olduğu belirlenmiştir. H1(0 h), H2 (0 h), H3 (0 h) ve H3 (24 h) uygulamalarında çok kaliteli kökler gözlenmiştir. Bu uygulamalar P. sempervivoides sukulent bitkisinin yaprak çeliklerinin köklendirilmesi için önerilebilir. Ayrıca P. sempervivoides'in yaprak çelikleri kullanılarak vejetatif çoğaltma yöntemiyle çoğaltılabileceği sonucuna varılmıştır.

Keywords

P. sempervivoides, köklenme, Aloe vera, yabani çiçek, süs bitkileri, hormon

Vegetative Propagation of Wild Prometheum sempervivoides (Fischer Ex M. Bieb.) H. Ohba by Leaf Cutting: Effects of Auxin and Some Substances

Abstract

The aim of the research is to determine the effects of different rooting contents [control (water), plant growth regulator-rooting hormone (H1: 0.25 g l-1, H2: 0.50 g l-1 and H3: 1.0 g l-1), cinnamon powder (T) and Aloe vera gel (AV)] and two different planting times on the rooting of leaf cuttings prepared from the stock mother plants of wild Prometheum sempervivoides (Fisch. ex Bieb.) H. Ohba taxon. Observations and measurements of vitality rate (%), number of rooted cuttings (NR), number of callusing cuttings (NC), maximum root length (MRL), rooting rate (%) and rooting scale (1-5) were made on cuttings whose rooting was completed. The results were evaluated statistically. The most positive rooting effect was determined in the H3 application, which is the highest dose of the commercial rooting hormone tested in the study. Cinnamon powder application was determined as a more effective natural extract for rooting leaf cuttings of P. sempervivoides when compared to Aloe vera gel application. At the end of our study, in which the effects of different planting times were also examined, it was determined that planting the leaf cuttings of P. sempervivoides as soon as they were taken from the stock mother plant had a positive effect on rooting. The very good quality roots were observed in H1 (0 h), H2 (0 h), H3 (0 h) and H3 (24 h) applications. These applications can be recommended for rooting the leaf cuttings of P. sempervivoides succulent plant. It was also concluded that P. sempervivoides can be reproduced by vegetative propagation method using leaf cuttings.

Keywords

P. sempervivoides, rooting, Aleo vera, wild flower, ornamental plants, hormone

References

• Al-Khafajy, R.A., AL-Taey, K. A., & AL-Mohammed, M. H. (2020). The impact of water quality, bio fertilizers and selenium spraying on some vegetative and flowering growth parameters of Calendula officinalis L. under salinity stress. International Journal of Agricultural and Statistical Sciences, 16, 1175-1180.
• Anton, D., & Cristescu, I. M. (2009). Investigations regarding the rooting of the cuttings belonging to some species of succulents, flowery plants. Journal of Horticulture, Forestry and Biotechnology, 13, 255-259.
• Arslan, M. (2010). The possibilities of using medicinal and aromatic plant species in landscape architecture studies. IV. Ornamental Plants Congress, Proceedings 20 – 22 October 2010, Erdemli/Mersin, p.265-272.
• Bhattacharya, A. (2019). Global climate change and its impact on agriculture. Changing Climate and Resource Use Efficiency in Plants; Academic Press: Cambridge, MA, USA, 1-50.
• Cabahug, R.A.M., Nam, S.Y., Lim, K.B., Jeon, J.K., & Hwang, Y.J. (2018). Propagation techniques for ornamental succulents. Flower Research Journal, 26(3), 90-101.
• Cabahug, R.A., Soh, S.Y., & Nam, S.Y. (2016a). Growth of Crassulaceae succulents as influenced by leaf cutting type and planting position. 화훼연구, 24(4), 255-263.
• Cabahug, R.A., Soh, S.Y., & Nam, S.Y. (2016b). Effects of auxin and cytokinin application on leaf cutting propagation in Echeveria species. 화훼연구, 24(4), 264-273.
• Chaudhari, B.B., Bhatt, D., Chawla, S.L., Patel, M.A., & Bennurmath, P. (2018). Effect of rooting hormone and media on root induction in poinsettia (Euphorbia pulcherrima Willd.). Journal of Ornamental Horticulture, 21(1and2), 7-12.
• Dilaver, Z. (2001). A research on the evaluation of the usability of natural vegetation samples in Ayaş Bel and its surroundings in landscape architecture studies. Unpublished doctoral thesis. Ankara University Institute of Science and Technology, Department of Landscape Architecture, Ankara, p. 318.
• Dilaver, Z., Öztekin M., & Yılmaz, M. (2020). Evaluation of ornamental plant characteristics of some natural taxa in Soğuksu National Park. Bursa Uludag Univ. Faculty of Agriculture Journal, 34, 197-215.
• Ekici, E. (2020). Investigations on vegetative propagation of Çobankavurgası (Sedum album L.) which is distributed in Kırşehir natural flora. Master's thesis, Kırşehir Ahi Evran University / Institute of Science and Technology / Department of Agricultural Biotechnology, 55 p.
• El-Sherif, F. (2017). Aloe vera leaf extract as a potential growth enhancer for Populus trees grown under in vitro conditions. American Journal of Plant Biology, 2(3), 101-105.
• Erduran, F., Çelik, A., & Özel Cengiz, A. E. (2010). The use of some woody plants in the flora of Çanakkale and Kazdağları in landscape architecture. IV. Ornamental Plants Congress, Proceedings 20 – 22 October 2010, Erdemli/Mersin, p. 463-470.
• Gunjan, S., & Anart, R.N. (2009). Influence of explants type and plant growth regulators on in vitro multiple shoots regeneration of laurel from Himalaya. Nature and Science, 7(9), 1-7.
• Gülbağ, F. (2016). Cultivation of some Campanula L. species and determination of ornamental plant characteristics. Unpublished doctoral thesis. Ege University, Graduate School of Natural and Applied Sciences, Department of Horticulture, İzmir, p.234.
• Hameed, R.L., & Adil, A.M. (2019). Effect of wounding, auxins and cinnamon extract on the rooting and vegetative growth characteristics of bottle brush plant (Melaleuca viminalis L.) cuttings. Scientific Journal of Flowers and Ornamental Plants, 6(2), 105-111.
• Hamouda, A.M.A., Hendi, D.M.G., & Abu-El-Leel, O.F.A. (2012). Improving basil growth, yield and oil production by Aloe vera extract and active dry yeast. Egyptian Journal of Horticulture (EJOH), 39, 45-71.
• Hamza, O.M., & AL-Taey, D.K.A. (2020). A study on the effect of glutamic acid and benzyl adenine application up on growth and yield parameters and active components of two Broccoli hybrids. Int. J. Agricult. Stat. Sci., 16(Supplement 1): 1163-1167. DocID: https:/ /connectjournals.com/ 03899.2020.16 .1163.
• Hasan, A.M., Mohamed, Ali T.J., & Al-Taey, D.K.A. (2019). Effects of winter foliar fertilizing and plant growth promoters on element and carbohydrate contents on the shoot of Navel Orange sapling. International Journal of Fruit Science, 19(1), 1-10.
• Hatfield, J.L., & Dold, C. (2019). Water-use efficiency: advances and challenges in a changing climate. Frontiers in Plant Science, 10, 103.
• Hodzic, J. (2020). Plant propagation protocol for Sedum oreganum ESRM 412 – Native Plant Production, https://courses.washington.edu/esrm412/protocols/SEOR.pdf, [Ziyaret tarihi: 03.12.2020].
• Ibrahim, M.E., Mohamed, M.A., & Khalid, K.A. (2015). Effect of plant growth regulators on the rooting of lemon verbena cutting. Journal of Materials and Environmental Science, 6(1), 28-33.
• Jeong, J.H. (1999). Influence of several factors on the rooting of Sedum rotundifolium stem and leaf cuttings. Journal of Korean Society of Horticultural Science, 40, 631-634.
• Khalid, W.K., & Ahmed, A.A. (2022). Study of some natural substances in rooting of two fig varieties. International Journal of Agricultural and Statistical Sciences, 18(1), 183-188.
• Khayru, R.K. (2021). Opinions about consumer behavior during the Covid-19 Pandemic. Journal of Social Science Studies (JOS3), 1(1), 31-36.
• Tahat, M., Alananbeh, K., Othman, Y., & Leskovar, D. (2020). Soil health and sustainable agriculture. Sustainability 12: 4859. Excessive and Disproportionate Use of Chemicals Cause Soil Contamination and Nutritional… DOI: http://dx. doi.org/10.5772/intechopen, 94593.
• Mihaela, C., Doina, A., Carmen, N., & Manuela, M. (2011). Research on the influence of the sampling priods on the propagation to cuttings at some succulent plants. Journal of Horticulture, Forestry and Biotechnology, 15, 109-114. Mirihagalla, M.K.P.N., & Fernando, K.M.C. (2020). Effect of Aloe vera gel for inducing rooting of stem cuttings and air layering of plants. Journal of Dry Zone Agriculture, 6, 13-26.
• Parlakova Karagöz, F., Karagöz, H., & Dursun, A. (2020). Properties and importance of Prometheum sempervivoides (Fisch. Ex Bieb.) H. Ohba as ornamental plant naturally grown in Erzurum. Journal of Agricultural Production, 1(1), 22-30.
• Paterson, K.E., & Rost, T.L. (1978). Effects of light and hormones on regeneration of Crassula argentea from leaves. American Journal of Botany, 66, 463-469.
• Rajan, R.P., & Singh, G. (2021). A review on the use of organic rooting substances for propagation of horticulture crops. Plant Archives, 21(1), 685-692.
• Sarı, D. (2021). Türkiye florasında yayılış gösteren crassulaceae familyasından bazı türlerin peyzaj ve süs bitkisi nitelikleri bakımından incelenmesi. In: Cengizler İ. & Duman S. (Eds). Ziraat, Orman ve Su Ürünlerinde Araştırma ve Değerlendirmeler – I. Gece Publishing, pp: 291-314.
• Sezgin, M., & Kahya, M. (2018). Phytohormones. Bitlis Eren University Journal of Science and Technology, 8(1), 35-39. Shidiki, A.A., Ambebe, T.F., & Mendi, A.G. (2019). A comparative evaluation of Indole-3- Butyric Acid and plant extracts as potential rooting enhancers in cuttings of Vitex diversifolia and Cordia milleneii. International Journal of Forest, Animal and Fisheries Research, 3(4).
• Shield, P. (2012). Three organic alternatives to hormone rooting powder. Retrieved from HTTP:// montrouchorga n ic. over-b log . com/article-three-organic alternatives - to - hormone - rooting - powder - 101172112.html Surjushe, A., Vasani, R., & Saple, D. (2008). Aloe vera: A short review. Indian Journal of Dermatology, 53(4): 163-166.
• Uddin A.J., Rakibuzzaman M., Raisa I., Maliha M., Husna M.A. 2020. Impact of natural substances and synthetic hormone on grapevine cutting. Journal of Bioscience and Agriculture Research, 25(01), 2069-2074.
• URL-1. Tips on succulent propagation from leaves and cuttings - Succulents Box (Access date: 02.11.2022). Wang, Q., Chen, J., Stamps, R.H., & Li, Y. (2005). Correlation of visual quality grading and SPAD reading of green-leaved foliage plants. Journal of Plant Nutrition, 28, 1215-1225.
• Xing, Y., Li, X., Xu, Q., Yun, J., & Lu, Y. (2010). Antifungal activities of cinnamon oil against Rhizopus nigricans, Aspergillus flavus and Penicillium expansum in vitro and in vivo fruit test. International Journal of Food Science & Technology, 45(9), 1837- 1842.
• Zhao, W., Liu, L., Shen, Q., Yang, J., Han, X., Tian, F., & Wu, J. (2020). Effects of water stress on photosynthesis, yield, and water use efficiency in winter wheat. Water, 12(8),2127.