Gökkuşağı Alabalığı (Oncorhynchus mykiss) Anaçlarında Açlık Döngüsü ve Yeniden Beslemenin Gamet Kalitesi ve Döllenme Üzerine Etkisi

Year 2024, Volume: 55 Issue: 3, 142 - 150, 29.09.2024

Nijat Nazarli , Güneş Yamaner

https://doi.org/10.17097/agricultureatauni.1457055

Abstract

Bu çalışmada, açlık ve yeniden besleme rejimi uygulanan anaçlarda, bu besleme düzeninin gamet kalitesi ve döllenme üzerindeki etkisi incelenmiştir. Kontrol grubu balıkları her gün beslenirken, deneme grubunun erkek ve dişi bireyleri bir hafta beslenmiş, bir hafta aç bırakılmıştır. Ağırlık artışı en yüksek kontrol grubunda görülmüşken; yemden yararlanma oranı tüm gruplarda normal düzeyde bulunmuştur (%0,9-1,3). Relatif fekondite kontrol grubu dişilerinde 935±62 yumurta/kg; bir hafta açlık rejimi uygulanan balıklarda ise 1317±241 yumurta/kg olarak bulunmuştur (p < ,05). En düşük yumurta çapı bir hafta aç bırakılan grupta elde edilmiştir (3,36±0,2 mm). Sperm hacmi dışında tüm spermatolojik parametreler gruplar arasında benzerlik göstermiştir. Sperm miktarı bir hafta açlık uygulanan balıklarda (46,9±20 ml) kontrol grubuna nazaran daha yüksek bulunmuştur. Erkek bireylere dayalı döllenme çalışmasında kontrol dişi ile yapılan döllenme sonuçları deneme grubunun erkek bireyleri için benzerlik göstermiş, en yüksek döllenme oranı kontrol dişisi ve kontrol erkeği kullanılarak yapılan döllenmede görülmüştür.

Keywords

Damızlık balık besleme, Yumurta kalitesi, Üreme performansı

Ethical Statement

Bu çalışma, hayvan deneyi İstanbul Üniversitesi Yerel Etik Kurulu tarafından onaylandıktan sonra gerçekleştirildi (Karar numarası: 2022/15).

Supporting Institution

Bu çalışma İstanbul Üniversitesi Bilimsel Araştırma Projeleri Koordinatörlüğü tarafından desteklenmiştir [Proje numarası: FYL-2022-38913].

Project Number

FYL-2022-38913

Thanks

Bu çalışma, İstanbul Üniversitesi Fen Bilimleri Enstitüsü'nde 2022 yılında tamamlanan yüksek lisans tezinden türetilmiştir. Yazarlar, desteklerinden dolayı İstanbul Üniversitesi Su Bilimleri Fakültesi Sapanca İç Sular Araştırma Merkezi yönetimine ve çalışanlarına içten şükranlarını sunar. Yazarlar ayrıca Doç. Dr. Aygül EKİCİ, Dr. Özgür ÇANAK, Dr. Rahmi Can ÖZDEMİR, doktora öğrencileri A. Mohammed Hassan AHMED, Muhammed Hanif AZHAR ve yüksek lisans öğrencileri K. Saheed OLUWASOLA ve J. Antonio ASPILCUETA VASQUEZ 'e içten teşekkürlerini sunarlar.

Effect of Starvation and Refeeding on Gamete Quality and Fertilization in Rainbow Trout (Oncorhynchus mykiss) Broodstock

Abstract

In this study, the impact of starvation and refeeding on broodstocks was observed by examining quality parameters of gametes and fertilization. While the control group fish were fed every day, the male and female members of the group were fed one week apart and placed in starvation after a week of feeding. Weight gain in the control group was the highest. The feed conversion ratio was normal in all groups (0.9-1.3%). Relative fecundity was (935±62 eggs/kg) in control group females; it was found to be (1317±241 eggs/kg) in starving females and statistically different in the groups (p < .05). The egg diameter of the group receiving intermittent feeding for a week (3.36±0.2 mm) was found to be the lowest. All spermatological parameters were similar between groups, except for sperm volume. The most sperm count was seen in the group that received one-week intermittent feeding (46.9± 20 ml). The results of fertilization with the control female in the fertilization study based on male individuals showed similarity for the male individuals of the trial group, the highest fertilization rate was seen in fertilization using the control female and control male.

Keywords

Broodfish feeding, Egg quality, Reproductive performance

Ethical Statement

This study was carried out after the animal experiment was approved by İstanbul University Local Ethics Committee (Decision number: 2022/15).

Supporting Institution

This work was supported by Scientific Research Projects Coordination Unit of Istanbul University [Project number: FYL-2022-38913].

Project Number

FYL-2022-38913

Thanks

This study was derived from a Master's thesis completed at Istanbul University, Institute of Graduate Studies in Sciences in 2022. The authors express their sincere gratitude to the management and staff of Sapanca Inland Waters Research Center of the Faculty of Aquatic Sciences, Istanbul University, Turkey for their support for their support. In addition, the authors would like to express their sincere thanks to Associate Professor Aygül EKİCİ, Dr. Özgür ÇANAK, Dr. Rahmi Can ÖZDEMİR, PhD student A. Mohammed Hassan AHMED; Muhammed Hanif AZHAR, and M.Sc. students K. Saheed OLUWASOLA and J. Antonio ASPILCUETA VASQUEZ.

References

• Alavi, S.M.H., Linhart, O., Coward, K., & Rodina, M. (2008). Fish Spermatology: Implications for Aquaculture Management. Fish Spermatology. 397-460. In: Alavi, S.M.H., Cosson, J.J., Coward, K., Rafiee, G. (Eds), Alpha Science International Ltd. Oxford, U.K.
• ANSCI, (2017). Determining the Concentration of Sperm with a Hemocytometer. http://www.ansci.wisc.edu/jj-p1/ansci_repro/lab/procedures/hemacytometer/Hemocytometer%2520use.html
• Bromage, N., (1995). Broodstock management and seed quality-general considerations.1-24. In: Roberts, R.J, Bromage, N.R. (Eds) Broodstock management and Egg and Larval Quality. Blackwell Science, Cambridge University Press Cambridge.
• Bromley, P.J., Ravier, C., & Witthames, P.R., (2000). The influence of feeding regime on sexual maturation, fecundity and atresia in first‐time spawning turbot. Journal of Fish Biology, 56(2), 264-278.
• Cardona, E., Bugeon, J., Guivarc'h, F., Goardon, L., Panserat, S., Labbé, L., & Bobe, J. (2019). Positive impact of moderate food restriction on reproductive success of the rainbow trout Oncorhynchus mykiss. Aquaculture, 502, 280-288.
• Caldwell, L.K., Pierce, A.L., Riley, L.G., Duncan, C.A., & Nagler, J.J. (2014). Plasma nesfatin-1 is not affected by long-term food restriction and does not predict rematuration among iteroparous female rainbow trout (Oncorhynchus mykiss). PLoS One, 9, 1.
• Carrillo, M., Zanuy, S., Oyen, F., Cerdà, J., Navas, JM., & Ramos, J. (2000). Some criteria of the quality of the progeny as indicators of physiological broodstock fitness. Recent advances in Mediterranean aquaculture finfish species diversification, 47, 61-73.
• Cerdá, J., Carrillo, M., Zanuy, S., & Ramos, J. (1994). Effect of food ration on estrogen and vitellogenin plasma levels, fecundity and larval survival in captive sea bass, Dicentrarchus labrax: preliminary observations. Aquatic Living Resources, 7(4), 255-266.
• Cleveland, B. M., Kenney, P. B., Manor, M. L., & Weber, G. M. (2012). Effects of feeding level and sexual maturation on carcass and fillet characteristics and indices of protein degradation in rainbow trout (Oncorhynchus mykiss). Aquaculture, 338, 228-236.
• Coward, K., & Bromage, N. R. (1999). Spawning frequency, fecundity, egg size and ovarian histology in groups of Tilapia zillii maintained upon two distinct food ration sizes from first-feeding to sexual maturity. Aquatic Living Resources, 12(1), 11-22.
• Davis, D. A. (Ed.). (2022). Feed and feeding practices in aquaculture. Woodhead publishing, Cambridge.
• Dziewulska, K., Rzemieniecki, A., & Domagała, J. (2008). Basic physico‐chemical parameters of milt from sea trout (Salmo trutta m. trutta), brook trout (Salvelinus fontinalis) and rainbow trout (Oncorhynchus mykiss). Journal of Applied Ichthyology, 24(4), 497-502.
• Ekici, A., Baran, A., Yamaner, G., Özdaş, Ö. B., Sandal, A. İ., Güven, E., & Baltacı, M. A. (2012). Effects of different doses of taurine in the glucose-based extender during cryopreservation of rainbow trout (Oncorhynchus mykiss) semen. Biotechnology & Biotechnological Equipment, 26(4), 3113-3115.
• Ekici, A., Baran, A., Özdaş, Ö. B., Sandal, A. İ., Yamaner, G., Güven, E., & Baltacı, M. A. (2014). The effect of streptomycin on freezing rainbow trout (Oncorhynchus mykiss) sperm. Israeli Journal of Aquaculture-Bamidgeh, 66.
• Erbaş, H. İ., & Başçınar, N. (2013). Yemleme sıklığının Karadeniz Alabalığı (Salmo trutta labrax Pallas, 1811)’nın sperm ve yumurta kalitesine etkisinin belirlenmesi. Biyoloji Bilimleri Araştırma Dergisi, 6(1), 11-16.
• Erbaş, H. İ., Başçınar, N., Sonay, F. D., & Civelek, R. O. (2013). Farklı büyüklükteki Karadeniz alabalığı (Salmo trutta labrax Pallas, 1811) damızlıklarının büyüme performansına yemleme sıklığının etkisi. Biyoloji Bilimleri Araştırma Dergisi, 6(1), 67-71.
• Gelinçek, İ., & Yamaner, G. (2020). An investigation on the gamete quality of Black Sea trout (Salmo trutta labrax) broodstock fed with mealworm (Tenebrio molitor). Aquaculture Research, 51(6), 2379-2388.
• Gunnes, K., & Gjedrem, T. (1978). Selection experiments with salmon: IV. Growth of Atlantic salmon during two years in the sea. Aquaculture, 15(1), 19-33.
• Imsland, A. K., & Gunnarsson, S. (2011). Growth and maturation in Arctic charr (Salvelinus alpinus) in response to different feed rations. Aquaculture, 318(3-4), 407-411.
• Izquierdo, M. S., Fernandez-Palacios, H., & Tacon, A. G. J. (2001). Effect of broodstock nutrition on reproductive performance of fish. Aquaculture, 197(1-4), 25-42.
• Hunter, J. R., Lo, N. C., & Leong, R. J. (1985). Batch fecundity in multiple spawning fishes. NOAA Technical Report Nmfs, 36, 67-77.
• Hopkins, C. L., & Unwin, M. J. (1997). The effect of restricted springtime feeding on growth and maturation of freshwater‐reared Chinook salmon, Oncorhynchus tshawytscha (Walbaum). Aquaculture Research, 28(7), 545-549.
• Lahnsteiner, F. (2003). Morphology, fine structure, biochemistry, and function of the spermatic ducts in marine fish. Tissue and Cell, 35(5), 363-373.
• Mañanós, E., Duncan, N., & Mylonas, C. (2009). Reproduction and control of ovulation, spermiation and spawning in cultured fish. Methods in reproductive aquaculture: Marine and freshwater species, 3-80.
• Reimers, E., Kjørrefjord, A. G., & Stavøstrand, S. M. (1993). Compensatory growth and reduced maturation in second sea winter farmed Atlantic salmon following starvation in February and March. Journal of Fish Biology, 43(5), 805-810.
• Chatzifotis, S., Papadaki, M., Despoti, S., Roufidou, C., & Antonopoulou, E. (2011). Effect of starvation and re-feeding on reproductive indices, body weight, plasma metabolites and oxidative enzymes of sea bass (Dicentrarchus labrax). Aquaculture, 316(1-4), 53-59.
• Reading, B. J., Andersen, L. K., Ryu, Y. W., Mushirobira, Y., Todo, T., & Hiramatsu, N. (2018). Oogenesis and egg quality in finfish: yolk formation and other factors influencing female fertility. Fishes, 3(4), 45.
• Ricker, W. E. (1979). Growth rates and models. Fish physiology, In: Hoar, W. S., Randall, D. J. and Brett, J. R. (Eds.), Fish physiology, Academic Press, New York, NY.
• Ridelman, J. M., Hardy, R. W., & Brannon, E. L. (1984). The effect of short-term starvation on ovarian development and egg viability in rainbow trout (Salmo gairdneri). Aquaculture, 37(2), 133-140.
• Sohn, Y. C., Yoshiura, Y., Kobayashi, M., & Aida, K. (1998). Effects of water temperature and food limitation on pituitary gonadotropin and thyrotropin subunit mRNA levels in the female goldfish Carassius auratus. Fisheries science, 64(5), 700-706.
• Sonay, FD. (2008): Karadeniz Alabalığı (Salmo trutta labrax Pallas, 1811)’nda ebeveynlerin döllenme oranı, kuluçkarandımanı, larva ve yavru gelişimi üzerine etkileri [Yüksek Lisans Tezi], Rize Üniversitesi.
• Taranger, G. L., Carrillo, M., Schulz, R. W., Fontaine, P., Zanuy, S., Felip, A., ... & Hansen, T. (2010). Control of puberty in farmed fish. General and comparative endocrinology, 165(3), 483-515.
• Tekin, N., Secer, S., Akcay, E., Bozkurt, Y., & Kayam, S. (2007). Effects of glycerol additions on post‐thaw fertility of frozen rainbow trout sperm, with an emphasis on interaction between extender and cryoprotectant. Journal of Applied Ichthyology, 23(1), 60-63.
• Tyler, C. R., Sumpter, J. P., & Witthames, P. R. (1990). The dynamics of oocyte growth during vitellogenesis in the rainbow trout (Oncorhynchus mykiss). Biology of Reproduction, 43(2), 202-209.
• Townshend, T. J., & Wootton, R. J. (1984). Effects of food supply on the reproduction of the convict cichlid, Cichlasoma nigrofasciatum. Journal of Fish Biology, 24(1), 91-104.
• Jobling, M., Jørgensen, E. H., & Siikavuopio, S. I. (1993). The influence of previous feeding regime on the compensatory growth response of maturing and immature Arctic charr, Salvelinus alpinus. Journal of Fish Biology, 43(3), 409-419.
• Thorpe, J. E., Talbot, C., Miles, M. S., & Keay, D. S. (1990). Control of maturation in cultured Atlantic salmon, Salmo salar, in pumped seawater tanks, by restricting food intake. Aquaculture, 86(2-3), 315-326.
• Yıldız, M., Ofori-Mensah, S., Arslan, M., Ekici, A., Yamaner, G., Baltacı, M. A., ... & Korkmaz, F. (2020). Effects of different dietary oils on egg quality and reproductive performance in rainbow trout Oncorhynchus mykiss. Animal Reproduction Science, 221, 106545.
• Yıldız, M., Ofori‐Mensah, S., Arslan, M., Yamaner, G., Ekici, A., Baltacı, M. A., ... & Tacer‐Tanas, Ş. (2021). Effects of different dietary lipid resources on sperm quality and reproductive success in rainbow trout (Oncorhynchus mykiss). Aquaculture Research, 52(8), 3804-3814.