Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne

Abstract

Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne, an aquatic plant belonging to the family Lythraceae, is used for the treatment of some diseases due to its medical and anti-microbial properties. This study presents multiple shoot regeneration from shoot tip and nodal explants of R. rotundifolia cultured on Murashige and Skoog (MS) nutrient medium containing 0.05- 1.25 mg/L Kinetin (KIN) and 0.25 mg/L Gibberellic acid (GA3) combinations for eight weeks. At the end of the second week, shoot formations began to be observed on the explants. High shoot regeneration frequencies were determined for both explants in the culture medium. The maximum number of shoots per explant was obtained from shoot tip (38.66) and nodal (30.77) explants cultured on MS medium containing 0.25 mg/L KIN + 0.25 mg/L GA3. Whereas the minimum number of shoots per explant was determined on MS medium containing 1.25 mg/L KIN + 0.25 mg/L GA3 for both explant types. The highest shoot lengths for shoot tip (1.87 cm) and nodal (1.79 cm) explants were obtained on MS culture medium containing 0.75 mg/L KIN + 0.25 mg/L GA3 and 0.50 mg/L KIN + 0.25 mg/L GA3, respectively. For in vitro rooting of the regenerated shoots, 2 cm long cut shoots were transferred to MS medium containing 0.25 mg/L indole-3-butyric acid (IBA). The rooted shoots were then successfully acclimatized to external conditions in the aquarium environment.

Keywords

• In vitro
• Kinetin
• R. rotundifolia
• Shoot regeneration
• Tissue culture

Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne’nın Sürgün Ucu ve Boğum Eksplantlarından Çoklu Sürgün Rejenerasyonu

Abstract

Lythraceae familyasına ait bir su bitkisi olan Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne, tıbbi ve antimikrobiyal özelliklerinden dolayı bazı hastalıkların tedavisinde kullanılır. Bu çalışma, 0,05-1,25 mg/L Kinetin (KIN) ve 0,25 mg/L Gibberellik asit (GA3) kombinasyonları içeren Murashige ve Skoog (MS) besin ortamında sekiz hafta boyunca kültüre alınan R. rotundifolia'nın sürgün ucu ve boğum eksplantlarından çoklu sürgün rejenerasyonunu sunmaktadır. İkinci haftanın sonunda explantlar üzerinde sürgün oluşumları gözlemlenmeye başlanmıştır. Kültür ortamında her iki eksplant için yüksek sürgün rejenerasyon oranları belirlenmiştir. Maksimum eksplant başına sürgün sayısı, 0,25 mg/L KIN + 0,25 mg/L GA3 içeren MS ortamında kültüre alınan sürgün ucu (38,66) ve boğum (30,77) eksplantlarından elde edilmiştir. Buna karşın, her iki eksplant türü için minimum eksplant başına sürgün sayısı 1,25 mg/L KIN + 0,25 mg/L GA3 içeren MS ortamında belirlenmiştir. Sürgün ucu (1,87 cm) ve boğum (1,79 cm) eksplantları için en yüksek sürgün uzunlukları sırasıyla 0,75 mg/L KIN + 0,25 mg/L GA3 ve 0,50 mg/L KIN + 0,25 mg/L GA3 içeren MS kültür ortamında elde edilmiştir. Rejenere sürgünlerin in vitro köklendirilmesi için, 2 cm uzunluğunda kesilen sürgünler 0,25 mg/L indol-3-bütirik asit (IBA) içeren MS ortamına aktarılmıştır. Köklü sürgünler daha sonra akvaryum ortamında dış koşullara başarılı bir şekilde alıştırılmıştır.

Keywords

• In vitro
• Kinetin
• R. rotundifolia
• Sürgün rejenerasyonu
• Doku kültürü

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