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Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne

Yıl 2017, , 4 - 8, 25.10.2017
https://doi.org/10.30616/ajb.343304

Öz

Rotala
rotundifolia
(Buch-Ham. ex Roxb) Koehne, an
aquatic plant belonging to the family Lythraceae, is used for the treatment of some
diseases due to its medical and anti-microbial properties. This study presents
multiple shoot regeneration from shoot tip and nodal explants of R.
rotundifolia
cultured on Murashige and Skoog (MS) nutrient medium
containing 0.05-1.25 mg/L Kinetin (KIN) and 0.25 mg/L Gibberellic acid (GA3)
combinations for eight weeks. At the end of the second week, shoot formations
began to be observed on the explants. High shoot regeneration frequencies were
determined for both explants in the culture medium. The maximum
number
of
shoots per explant was obtained from shoot tip (38.66)
and nodal (30.77) explants cultured on MS medium containing 0.25 mg/L KIN +
0.25 mg/L GA3. Whereas the minimum number of shoots per explant was determined
on MS medium containing 1.25 mg/L KIN + 0.25 mg/L GA3 for both
explant types.The highest shoot lengths for shoot tip (1.87 cm) and
nodal (1.79 cm) explants were obtained on MS culture medium containing 0.75
mg/L KIN + 0.25 mg/L GA3 and 0.50 mg/L KIN + 0.25 mg/L GA3,
respectively. For in vitro rooting of
the regenerated shoots, 2 cm long cut shoots were transferred to MS medium
containing 0.25 mg/L indole-3-butyric acid (IBA). The rooted shoots were then
successfully acclimatized to external conditions in the aquarium environment. 

Kaynakça

  • Banerjee M, Shrivastava S (2008). An Improved Protocol for In vitro Multiplication of Bacopa monnieri (L.). World J Microbiol Biotechnol 24: 1355-1359.
  • Bhattacharyya R, Bhattacharya S (2001). High Frequency In vitro Propagation of Phyllanthus amarus Schum. &Thom. by Shoot Tip Culture. Indian J Exp Biol 39: 1184-1187.
  • Bhowmik S, Saha M, Datta BK (2012). Extended Distribution of Rotala rotundifolia (Buch.-Ham. ex Roxb.) Koehne (Lyhthraceae) from India. NeBIO 3: 48-50. Bornette G, Puijalon S (2011). Response of Aquatic Plants to Abiotic Factors: a Review. Aquatic Sci 73: 1-14.
  • Dogan M, Emsen B, Aasim M, Yildirim E (2017). Ceratophyllum demersum L. Extract As a Botanical Insecticide for Controlling the Maize Weevil, Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae). Egypt J Biol Pest Control 27: 11-15.
  • Dogan M, Karatas M, Aasim M (2016). In vitro Shoot Regeneration From Shoot Tip And Nodal Segment Explants of Pogostemon erectus (Dalzell) Kuntze, A Multipurpose Ornamental Aquatic Plant. Fresen Environ Bull 25: 4777-4782.
  • Dogan M, Karatas M, Aasim M (2015). An Efficient In vitro Plantlet Regeneration of Ceratophyllum demersum L., an Important Medicinal Aquatic Plant. Fresen Environ Bull 24: 3499-3504.
  • Emsen B, Dogan M, Aasim M, Yildirim E (2016). Insecticidal Activity of In vitro Propagated Aquatic Plant Ceratophyllum demersum L. against Granary Weevil Sitophilus granarius L. (Coleoptera: Curculionidae). Egypt J Biol Pest Control 26: 619-624.
  • Fontanili L, Lucchesini M, Mensuali-Sodi A (2015). In vitro Propagation and Shoot Encapsulation as Tools For Ex Situ Conservation of the Aquatic Plant Ludwigia palustris (L.) Ell. Plant Biosyst 149: 855-864.
  • Gnanaraj WE, Marimuthu J, Subramanian KM, Nallyan S (2011). Micropropagation of Alternanthera sessilis (L.) Using Shoot Tip and Nodal Segments. Iran J Biotechnol 9: 206-212.
  • Gross EM, Johnson RL, Hairston NG Jr (2001). Experimental Evidence for Changes in Submersed Macrophyte Species Composition Caused by the Herbivore Acentria ephemerella (Lepidoptera). Oecologia 127: 105-114.
  • Ho YL, Huang SS, Deng JS, Lin YH, Chang YS, Huang GJ (2012). In vitro Antioxidant Properties and Total Phenolic Contents of Wetland Medicinal Plants in Taiwan. Bot Stud 53: 55-66.
  • Karatas M, Dogan M, Emsen B, Aasim M (2015). Determination of In vitro Free Radical Scavenging Activities of Various Extracts From In Vitro Propagated Ceratophyllum demersum L.. Fresen Environ Bull 24: 2946-2952.
  • Kaviani B, Hesar AA, Tarang A, Zanjani SB, Hashemabadi D, Ansari MH (2013). Effect of Kinetin (Kn) and Naphthalene Acetic Acid (NAA) on the Micropropagation of Matthiola incana Using Shoot Tips, and Callus Induction and Root Formation on the Leaf Explants. Afr J Agric Res 8: 4134-4139.
  • Manik SR, Yatoo GM, Ahmad Z, Nathar VN (2012). Direct Organogenesis of Mentha piperata L. from Shoot Tip, Nodal and Sucker Explants. J Agr Tech 8: 663-669.
  • Murashige T, Skoog F (1962). A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures. Physiol Plant 15: 473-497.
  • Oyedeji AA, Abowei JFN (2012). The Classification, Distribution, Control and Economic Importance of Aquatic Plants. Int J Fish and Aqua Sci 1:118-128.
  • Raja HD, Arockiasamy DI (2008). In vitro Propagation of Mentha viridis L. from Nodal and Shoot Tip Explants. Plant Tissue Cult Biotechnol 18: 1-6.
  • Shahzad A, Parveen S, Fatema M (2011). Development of a Regeneration System Via Nodal Segment Culture in Veronica anagallis-aquatica L. - An Amphibious Medicinal Plant. J Plant Interact 6: 61-68.
  • Snedecor GW, Cochran WG (1997). Statistical Methods. The Iowa State University Press, Iowa. USA.
  • Stanly C, Bhatt A, Keng CL (2011). An Efficient In vitro Plantlet Regeneration of Cryptocoryne wendtii and Cryptocoryne becketti through Shoot Tip Culture. Acta Physiol Plant 33: 619-624.
  • Swapna MM, Prakashkumar R, Anoop KP, Manju CN, Rajith NP (2011) A Review on the Medicinal and Edible Aspects of Aquatic and Wetland Plants of India. J Med Plants Res 5: 7163-7176.
  • Tan QG, Cai XH, Feng T, Luo XD (2009). Megastigmane-type Compounds from Rotala rotundifolia. Chin J Nat Med 7: 187-189.
  • Zhang LJ, Yeh SF, Yu YT, Kuo LMY, Kuo Dr YH (2011). Antioxidative Flavonol Glucuronides and Anti-HBsAg Flavonol from Rotala rotundifolia. J Tradit Complement Med 1: 57-63.
Yıl 2017, , 4 - 8, 25.10.2017
https://doi.org/10.30616/ajb.343304

Öz

Kaynakça

  • Banerjee M, Shrivastava S (2008). An Improved Protocol for In vitro Multiplication of Bacopa monnieri (L.). World J Microbiol Biotechnol 24: 1355-1359.
  • Bhattacharyya R, Bhattacharya S (2001). High Frequency In vitro Propagation of Phyllanthus amarus Schum. &Thom. by Shoot Tip Culture. Indian J Exp Biol 39: 1184-1187.
  • Bhowmik S, Saha M, Datta BK (2012). Extended Distribution of Rotala rotundifolia (Buch.-Ham. ex Roxb.) Koehne (Lyhthraceae) from India. NeBIO 3: 48-50. Bornette G, Puijalon S (2011). Response of Aquatic Plants to Abiotic Factors: a Review. Aquatic Sci 73: 1-14.
  • Dogan M, Emsen B, Aasim M, Yildirim E (2017). Ceratophyllum demersum L. Extract As a Botanical Insecticide for Controlling the Maize Weevil, Sitophilus zeamais Motschulsky (Coleoptera: Curculionidae). Egypt J Biol Pest Control 27: 11-15.
  • Dogan M, Karatas M, Aasim M (2016). In vitro Shoot Regeneration From Shoot Tip And Nodal Segment Explants of Pogostemon erectus (Dalzell) Kuntze, A Multipurpose Ornamental Aquatic Plant. Fresen Environ Bull 25: 4777-4782.
  • Dogan M, Karatas M, Aasim M (2015). An Efficient In vitro Plantlet Regeneration of Ceratophyllum demersum L., an Important Medicinal Aquatic Plant. Fresen Environ Bull 24: 3499-3504.
  • Emsen B, Dogan M, Aasim M, Yildirim E (2016). Insecticidal Activity of In vitro Propagated Aquatic Plant Ceratophyllum demersum L. against Granary Weevil Sitophilus granarius L. (Coleoptera: Curculionidae). Egypt J Biol Pest Control 26: 619-624.
  • Fontanili L, Lucchesini M, Mensuali-Sodi A (2015). In vitro Propagation and Shoot Encapsulation as Tools For Ex Situ Conservation of the Aquatic Plant Ludwigia palustris (L.) Ell. Plant Biosyst 149: 855-864.
  • Gnanaraj WE, Marimuthu J, Subramanian KM, Nallyan S (2011). Micropropagation of Alternanthera sessilis (L.) Using Shoot Tip and Nodal Segments. Iran J Biotechnol 9: 206-212.
  • Gross EM, Johnson RL, Hairston NG Jr (2001). Experimental Evidence for Changes in Submersed Macrophyte Species Composition Caused by the Herbivore Acentria ephemerella (Lepidoptera). Oecologia 127: 105-114.
  • Ho YL, Huang SS, Deng JS, Lin YH, Chang YS, Huang GJ (2012). In vitro Antioxidant Properties and Total Phenolic Contents of Wetland Medicinal Plants in Taiwan. Bot Stud 53: 55-66.
  • Karatas M, Dogan M, Emsen B, Aasim M (2015). Determination of In vitro Free Radical Scavenging Activities of Various Extracts From In Vitro Propagated Ceratophyllum demersum L.. Fresen Environ Bull 24: 2946-2952.
  • Kaviani B, Hesar AA, Tarang A, Zanjani SB, Hashemabadi D, Ansari MH (2013). Effect of Kinetin (Kn) and Naphthalene Acetic Acid (NAA) on the Micropropagation of Matthiola incana Using Shoot Tips, and Callus Induction and Root Formation on the Leaf Explants. Afr J Agric Res 8: 4134-4139.
  • Manik SR, Yatoo GM, Ahmad Z, Nathar VN (2012). Direct Organogenesis of Mentha piperata L. from Shoot Tip, Nodal and Sucker Explants. J Agr Tech 8: 663-669.
  • Murashige T, Skoog F (1962). A Revised Medium for Rapid Growth and Bioassays with Tobacco Tissue Cultures. Physiol Plant 15: 473-497.
  • Oyedeji AA, Abowei JFN (2012). The Classification, Distribution, Control and Economic Importance of Aquatic Plants. Int J Fish and Aqua Sci 1:118-128.
  • Raja HD, Arockiasamy DI (2008). In vitro Propagation of Mentha viridis L. from Nodal and Shoot Tip Explants. Plant Tissue Cult Biotechnol 18: 1-6.
  • Shahzad A, Parveen S, Fatema M (2011). Development of a Regeneration System Via Nodal Segment Culture in Veronica anagallis-aquatica L. - An Amphibious Medicinal Plant. J Plant Interact 6: 61-68.
  • Snedecor GW, Cochran WG (1997). Statistical Methods. The Iowa State University Press, Iowa. USA.
  • Stanly C, Bhatt A, Keng CL (2011). An Efficient In vitro Plantlet Regeneration of Cryptocoryne wendtii and Cryptocoryne becketti through Shoot Tip Culture. Acta Physiol Plant 33: 619-624.
  • Swapna MM, Prakashkumar R, Anoop KP, Manju CN, Rajith NP (2011) A Review on the Medicinal and Edible Aspects of Aquatic and Wetland Plants of India. J Med Plants Res 5: 7163-7176.
  • Tan QG, Cai XH, Feng T, Luo XD (2009). Megastigmane-type Compounds from Rotala rotundifolia. Chin J Nat Med 7: 187-189.
  • Zhang LJ, Yeh SF, Yu YT, Kuo LMY, Kuo Dr YH (2011). Antioxidative Flavonol Glucuronides and Anti-HBsAg Flavonol from Rotala rotundifolia. J Tradit Complement Med 1: 57-63.
Toplam 23 adet kaynakça vardır.

Ayrıntılar

Konular Yapısal Biyoloji
Bölüm Articles
Yazarlar

Muhammet Dogan

Yayımlanma Tarihi 25 Ekim 2017
Kabul Tarihi 16 Ekim 2017
Yayımlandığı Sayı Yıl 2017

Kaynak Göster

APA Dogan, M. (2017). Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne. Anatolian Journal of Botany, 1(1), 4-8. https://doi.org/10.30616/ajb.343304
AMA Dogan M. Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne. Ant J Bot. Ekim 2017;1(1):4-8. doi:10.30616/ajb.343304
Chicago Dogan, Muhammet. “Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala Rotundifolia (Buch-Ham. Ex Roxb) Koehne”. Anatolian Journal of Botany 1, sy. 1 (Ekim 2017): 4-8. https://doi.org/10.30616/ajb.343304.
EndNote Dogan M (01 Ekim 2017) Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne. Anatolian Journal of Botany 1 1 4–8.
IEEE M. Dogan, “Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne”, Ant J Bot, c. 1, sy. 1, ss. 4–8, 2017, doi: 10.30616/ajb.343304.
ISNAD Dogan, Muhammet. “Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala Rotundifolia (Buch-Ham. Ex Roxb) Koehne”. Anatolian Journal of Botany 1/1 (Ekim 2017), 4-8. https://doi.org/10.30616/ajb.343304.
JAMA Dogan M. Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne. Ant J Bot. 2017;1:4–8.
MLA Dogan, Muhammet. “Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala Rotundifolia (Buch-Ham. Ex Roxb) Koehne”. Anatolian Journal of Botany, c. 1, sy. 1, 2017, ss. 4-8, doi:10.30616/ajb.343304.
Vancouver Dogan M. Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne. Ant J Bot. 2017;1(1):4-8.

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