Multiple Shoot Regeneration from Shoot Tip and Nodal Explants of Rotala rotundifolia (Buch-Ham. ex Roxb) Koehne

Yıl 2017, , 4 - 8, 25.10.2017

Muhammet Dogan

https://doi.org/10.30616/ajb.343304

Cited By: 7

Öz

Rotala
rotundifolia (Buch-Ham. ex Roxb) Koehne, an
aquatic plant belonging to the family Lythraceae, is used for the treatment of some
diseases due to its medical and anti-microbial properties. This study presents
multiple shoot regeneration from shoot tip and nodal explants of R.
rotundifolia cultured on Murashige and Skoog (MS) nutrient medium
containing 0.05-1.25 mg/L Kinetin (KIN) and 0.25 mg/L Gibberellic acid (GA₃)
combinations for eight weeks. At the end of the second week, shoot formations
began to be observed on the explants. High shoot regeneration frequencies were
determined for both explants in the culture medium. The maximum number
of shoots per explant was obtained from shoot tip (38.66)
and nodal (30.77) explants cultured on MS medium containing 0.25 mg/L KIN +
0.25 mg/L GA₃. Whereas the minimum number of shoots per explant was determined
on MS medium containing 1.25 mg/L KIN + 0.25 mg/L GA₃ for both
explant types.The highest shoot lengths for shoot tip (1.87 cm) and
nodal (1.79 cm) explants were obtained on MS culture medium containing 0.75
mg/L KIN + 0.25 mg/L GA₃ and 0.50 mg/L KIN + 0.25 mg/L GA₃,
respectively. For in vitro rooting of
the regenerated shoots, 2 cm long cut shoots were transferred to MS medium
containing 0.25 mg/L indole-3-butyric acid (IBA). The rooted shoots were then
successfully acclimatized to external conditions in the aquarium environment. 

Anahtar Kelimeler

In vitro, Kinetin, R. rotundifolia, Shoot regeneration, Tissue culture

Kaynakça

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