In Vitro rapid propagation of an aquatic plant Pogostemon erectus (Dalzell) Kuntze

Abstract

The aim of this study is to investigate the multiple and rapid production of Pogostemon erectus (Dalzell) Kuntze by tissue culture techniques. The shoot tip and nodal explants of P. erectus were isolated and then cultured in Murashige and Skoog (1962) (MS) nutrient media containing thidiazuron (TDZ) and 2,4-Dichlorophenoxyacetic acid (2,4-D) in different combinations. The first shoot formation on the explants was observed on day 15. The number of shoots per explant ranged from 2.85 to 29.39 in the shoot tip explants and from 2.72 to 32.24 in the nodal explants. The maximum number of shoots per explant was obtained in the MS medium containing 0.20 mg/L TDZ + 0.10 mg/L 2,4-D for shoot tip explant (29.39) and in the MS medium containing 0.10 mg/L TDZ + 0.10 mg/L 2,4-D for nodal explant (32.24). The longest shoots in the shoot tip (1.76 cm) and nodal (1.64 cm) explants were determined in MS medium supplemented with 0.10 mg/L TDZ + 0.10 mg/L 2,4-D. Regenerated shoots were rooted in MS medium supplemented with 0.25 mg/L indol-3yl acetic acid (IAA), and then acclimatized to aquarium conditions.

Keywords

• In vitro
• Shoot regeneration
• TDZ
• Tissue culture
• Pogostemon erectus
• Shoot regeneration
• TDZ
• Tissue culture

Sucul bir bitki olan Pogostemon erectus (Dalzell) Kuntze’un In Vitro hızlı çoğaltımı

Abstract

Bu çalışmanın amacı, Pogostemon erectus (Dalzell) Kuntze'un doku kültürü teknikleri ile çoklu ve hızlı üretimini araştırmaktır. P. erectus'un sürgün ucu ve nodal eksplantları izole edilmiş ve daha sonra farklı kombinasyonlarda thidiazuron (TDZ) ve 2,4-Diklorofenoksiasetik asit (2,4-D) içeren Murashige ve Skoog (1962) (MS) besin ortamında kültüre alınmıştır. Eksplantlardaki ilk sürgün oluşumu 15. günde gözlenmiştir. Eksplant başına sürgün sayısı, sürgün ucu eksplantlarında 2.85 ila 29.39 ve nodal eksplantlarında 2.72 ila 32.24 arasında sıralanmıştır. Eksplant başına maksimum sürgün sayısı sürgün ucu eksplantı için (29.39) 0.20 mg/L TDZ + 0.10 mg/L 2,4-D içeren MS ortamında ve nodal eksplant için 0.10 mg/L TDZ + 0.10 mg/L 2,4-D içeren MS ortamında (32.24) elde edilmiştir. En uzun sürgünler, sürgün ucu (1.76 cm) ve nodal (1.64 cm) eksplantlarında 0.10 mg/L TDZ + 0.10 mg/L 2,4-D ile desteklenmiş MS ortamında belirlenmiştir. Rejenere sürgünler, 0.25 mg/L Indol-3-asetik asit (IAA) ile takviye edilmiş MS ortamında köklendirilmiş ve ardından akvaryum koşullarına alıştırılmıştır.

Keywords

• In vitro
• Sürgün rejenerasyonu
• TDZ
• Pogostemon erectus
• TDZ
• Doku kültürü

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