Comparative analysis of total phenolic and flavonoid content, antioxidant, and tyrosinase inhibitory properties of Silybum marianum L. from Iraq and Türkiye

Year 2025, Volume: 9 Issue: 2, 101 - 107

Nazl Shakir Shakir , Nuraniye Eruygur

https://doi.org/10.30616/ajb.1658938

Abstract

This study investigates the phytochemical composition and bioactive properties of Silybum marianum L. seeds collected from Iraq and Türkiye, two regions with a history of traditional medicinal use of this herb. Extracts were analyzed for total phenolic and flavonoid content (TPC and TFC) using colorimetric methods, and for antioxidant [2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) assays] and tyrosinase inhibitory activity. Results showed that Turkish extracts had significantly higher TPC and TFC (165.65 ± 6.99 mg GAE/g and 7.66 ± 0.71mg QE/g, respectively) compared to Iraqi extracts, which correlated with enhanced antioxidant and tyrosinase inhibition. The study underscores the impact of regional and environmental factors on the medicinal attributes of S. marianum L., offering valuable information for optimizing its application in the pharmaceutical and cosmetic industries. Turkey.

Keywords

Silybum marianum L. , antioxidant activity , phytochemical composition

Ethical Statement

The authors declare no conflicts of interest.

Supporting Institution

The authors extend their appreciation to the Selcuk University Scientific Research Foundation for funding this research work through the project number: SUBAP- 24202050.

Project Number

SUBAP- 24202050

Irak ve Türkiye'de kullanılan Silybum marianum L.'un toplam fenolik ve flavonoid içeriği, antioksidan ve tirozinaz inhibisyon aktivitelerinin karşılaştırmalı incelenmesi

Abstract

Bu çalışma, bu bitkinin geleneksel tıbbi kullanım geçmişine sahip iki bölge olan Irak ve Türkiye'den toplanan Silybum marianum L. tohumlarının fitokimyasal bileşimini ve biyoaktif özelliklerini araştırmaktadır. Ekstreler, kolorimetrik yöntemler kullanılarak total fenolik ve flavonoit içeriği (TPC ve TFC) ve antioksidan [2,2-difenil-1-pikrilhidrazil (DPPH) ve 2,2'-azino-bis(3-etilbenzotiyazolin-6-sülfonik asit) (ABTS) deneyleri] ve tirozinaz inhibitör aktivitesi için analiz edilmiştir. Sonuçlar, Türk ekstraktlarının Irak ekstraktlarına kıyasla önemli ölçüde daha yüksek TPC ve TFC'ye (sırasıyla 165.65 ± 6.99 mg GAE/g ve 7.66 ± 0.71mg QE/g) sahip olduğunu ve bunun da gelişmiş antioksidan ve tirozinaz inhibisyonu ile ilişkili olduğunu göstermiştir. Çalışma, bölgesel ve çevresel faktörlerin S. marianum L.'nin tıbbi özellikleri üzerindeki etkisinin altını çizmekte ve ilaç ve kozmetik endüstrilerindeki uygulamalarını optimize etmek için değerli bilgiler sunmaktadır.

Keywords

Silybum marianum L. , antioksidan aktivite , fitokimyasal bileşim

Project Number

SUBAP- 24202050

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