In Vitro Evaluation of Gilaburu (Viburnum Opulus L. ) Juice on Different Cell Lines

Year 2019, , 549 - 571, 01.07.2019

Ayşe Tansu Koparal

https://doi.org/10.18039/ajesi.577253

Cited By: 11

Abstract

The purpose of this
study is to investigate the effects of gilaburu juice on cell viability
(3-(4,5-dimethyl-thiazol-2-yl)-2,5-diphenyl tetrazolium bromide, MTT) and
angiogenesis (tube formation assay) using different cell lines (human cancer
cell lines A549, Caco-2, HeLa and normal cell lines MDCK and HUVEC) in vitro.
In addition, the genotoxic effects of gilaburu juice is evaluated using COMET
assay on HUVEC cells. Our results demonstrate that gilaburu juice could inhibit
the growth of Caco-2 and HeLa cancer cell lines, but could not significantly
inhibit normal cell lines and A549 cancer cell lines. It disrupted tube
formation of HUVEC cells. Gilaburu juice appears to have no genotoxic potential
to the DNA of HUVEC cells. The results obtained in this study confirm the
potential application of commercial gilaburu juice as a functional food in
prevention of cancer.

Keywords

Gilaburu juice, European cranberry bush, Viburnum opulus, angiogenesis, cancer

Thanks

The author thanks Edward Mcquaid for proofreading of the manuscript.

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