Investigation of Antifungal and Antibacterial Effect of Lavandula angustifolia Against Candida albicans and Escherichia coli

Year 2023, Volume: 5 Issue: 3, 121 - 128, 31.12.2023

Deyar Sami Khalif , Ahmet Özbek , Tuğçe Naime Gedik Kapancık , Zeynep Taner

Abstract

Essential oils are well-known antiseptics and antibacterial agents used in traditional medicine, possessing extensive activities, including tasks hostile to both bacteria and fungus. Infections caused by both Candida albicans and Escherichia coli are of high significant issue in medical management, since some strains have proven to be resistance to antibiotics. Our aim is to investigate the antimicrobial activity of the essential oil Lavandula angustifolia and its effect on both C. albicans ATCC 10231 and E. coli ATCC 25922 by examining its MIC, MFC, and MBC to investigate if it can be used as a supportive or protective natural complementary herbal medication. In this experiment, our C. albicans isolates were then taken subcultures and incubated at 37℃ for 24 hours, then different dilutions of Lavandula angustifolia were prepared as; 100%, 50%, 25%, 12.5%, and 6.5% diluted concentrations. Kirby- Bauer disc diffusion method was used to determine the resistance of Lavandula angustifolia against both C. albicans and E. coli, as well as broth microdilution method was used to determine the Minimal Inhibitory Concentration (MIC), Minimal Fungicidal Concentration (MFC) and Minimal Bactericidal Concentration (MBC). Comparing the resulted obtained showed that lavender had a significant antibiosis effect to both C. albicans and E. coli under different concentrations. Although, our results lead to establish that Lavandula angustifolia possess both antifungal and antibacterial activity, it is yet to be proven as for its use as both antifungal and antibacterial agent in medical care with more clinical studies necessary to validate it.

Keywords

Antimicrobial, Lavandula angustifolia, Candida albicans, Escherichia coli, MIC, MFC, MBC.

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