Molecular Chimerism Methods: Past and Present

Abstract

ABSTRACT Chimerism analysis is a diagnostic method used to determine the amount of donor cells in the patient for hematologic cases after allogeneic hematoietic stem cell transplantation (AHSCT). Post-transplant chimerism analysis provides very important information in terms of donor cell engraftment and relapse. Chimerism analysis is performed using various markers and techniques. Recently, new chimerism methods have been used such as quantitative-real time polymerase chain reaction (qRT-PCR), digital droplet-PCR (dd-PCR) and next generation sequencing (NGS). In this review, multiplex short tandem repeat based-PCR (STR-PCR) and new molecular methods qRT-PCR, dd-PCR and NGS were compared and discussed in the terms of advantageous and disadvantageous aspects. Key Words: Chimerism analysis, STR-PCR, qRT-PCR, dd-PCR, NGS

Keywords

• Chimerism analysis
• STR-PCR
• qRT-PCR
• dd-PCR
• NGS

Moleküler Kimerizm Metotları: Geçmiş ve Günümüz

Öz

ÖZ Kimerizm analizi, hematolojide allojenik hematopoetik kök hücre transplantasyonu (AHKHT) sonrası hastadaki donör hücre miktarının tespiti için kullanılan bir tanı yöntemidir. Transplantasyon sonrası yapılan kimerizm analizi, donör hücre engraftmanı ve hastalığın relapsı açısından oldukça önemli bilgiler vermektedir. Kimerizm analizi, çeşitli belirteçler ve teknikler kullanılarak yapılmaktadır. Son zamanlarda kantitatif-gerçek zamanlı polimeraz zincir reaksiyonu (qRT-PCR), dijital droplet-PCR (dd-PCR) ve yeni nesil dizileme (NGS) gibi yeni kimerizm yöntemleri kullanılmaktadır. Bu derlemede multipleks kısa ardışık tekrarlı dizi tabanlı PCR (STR-PCR) yöntemi ile yeni moleküler qRT-PCR, dd-PCR ve NGS yöntemlerinin avantajlı ve dezavantajlı yönleri karşılaştırılıp tartışılmıştır. Anahtar Sözcükler: Kimerizm analizi, STR-PCR, qRT-PCR, dd-PCR, NGS

Anahtar Kelimeler

• Kimerizm analizi
• STR-PCR
• qRT-PCR
• dd-PCR
• NGS

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