Akciğer Tüberkülozlu Hastaların Bronkoalveolar Lavajında İL-12, İFN-? ve sİL-2 Düzeyleri

Abstract

Amaç: Çalışmamızda lokal immün cevabı yansıttığı düşünülen sitokin seviyelerini İL-12, İFN-γ ve sİL-2 bronkoalveolar lavaj sıvısında ölçerek balgamda yayma negatif saptanan hastalar ile balgam yayması pozitif olan hastaları karşılaştırmayı amaçladık. Gereç ve Yöntemler: Çalışma retrospektif olarak dizayn edildi. Çalışmaya Ekim 1998-Şubat 1999 arasında Yedikule Göğüs Hastalıkları ve Göğüs Cerrahisi Eğitim ve Araştırma Hastanesi’nde tetkik ve tedavi edilen 24 tüberküloz hastası alındı. Kontrol grubu olarak diğer nedenlerle bronkoskopi yapılmış olan akciğer malignitesi veya tüberkülozu olmayan 15 hasta alındı. Bulgular: Hasta ve kontrol grupları İL-12, İFN-γ ve sİL-2 düzeyleri açısından karşılaştırıldığında İL-12 ve İFN-γ düzeylerinin anlamlı şekilde farklı olduğu tespit edildi sırasıyla p: 0,02 ve p

Keywords

• Bronkoalveolar lavaj, İnterferon, İnterlökinler, Tüberküloz

IL-12, IFN-γ and sIL-2 Levels in Bronchoalveolar Lavage of Patients with Pulmonary Tuberculosis

Abstract

Objective: We aimed to compare cytokine levels IL-12, IFN-γ and IL-2 , thought to reflect local immunologic response, in sputum smear positive and negative patients by measuring them in bronchoalveolar lavage fluid in this study.Material and Methods: The study had a retrospective design. A total of 24 tuberculosis patients who were examined and treated at Yedikule Chest Diseases and Chest Surgery Training and Research Hospital between October 1998 and February 1999 were included. Patients without pulmonary malignancy and tuberculosis who had undergone bronchoscopy for other reasons were included as the control group.Results: When the IL-12, IFN-γ and sIL-2 levels were compared in the patient and control groups, the IL-12 and IFN-γ levels were found to show a significant difference p: 0.02 and p

Keywords

• Bronchoalveolar lavage, Interferon, Interleukins, Tuberculosis

References

1. Wolf SF, Temple Pa, Kobayashi M, Young D, Dicig M, Lowe L, Dzialo R, Fitz L, Ferenz C, Hewick RM. Cloning of cDNA for natural killer cell stimulatory factor, a heterodimeric cytokine with multiple biologic effects on T and natural killer cells. J Immunol 1991; 146(9):3074-81.
2. Gubler U, Chua AO, Schoenhaut DS, Dwyer CM, McComas W, Motyka R, Nabavi N, Wolitzky AG, Quinn PM, Familletti PC. Coexpression of two distinct genes is required to generate secreted bioactive cytotoxic lymphocyte maturation factor. Proc Natl Acad Sci USA. 1991; 88(10):4143-7.
3. Boom WH, Toossi Z, Wolf SF. The modulation by IL- 2, NKSF (IL-12/CLMF) and TGF-Bof CD4+ T cell mediated cytotoxity for macrophages (abstract). In Proc Twenty-Seventh Joint Conf Tuberc Lepr 1992;163-7.
4. Zhang M, Gately MK, Wang E, Gong J, Wolf SF, Lu S, Modlin RL, Barnes PF. Interleukin 12 at the site of disease in tuberculosis. J Clin Invest 1994; 93(4):1733-9.
5. Nikaido T, Shimizu N, Ishida N, Sabe H, Teshigawara K, Maeda M, Uchiyama T, Yodoi J, Honjo T. Molecular cloning of cDNA encoding human IL-2 receptor. Nature 1984; 311:631-5.
6. Panek SJ, Boer JS. Diagnosis of pulmonary tuberculosis by flexible fiberoptic bienchoscopy. Am Rev Respir Dis 1979; 119:677-9.
7. Chan HS, Sun AJ, Hoheisel GB. Bronchoscopic aspiration and Bronchoalveolar lavage in diagnosis of sputum smear- negative pulmonary tuberculosis. Lung 1990; 168:215-20.
8. Dalton DK, Pitts-Meek S, Keshav S, Figari IS, Bradley A, Stewart TA. Multiple defect of immune cell function in mice with disrupted γ-interferon genes. Science 1993; 259:1739-42.

9. Huang S, Hendriks W, Althage A, Hemmi S, Bluethmann H, Kamijo R, Vilcek J, Zinkernagel RM, Aguet M. Immune response in mice that lack the interferon-γ receptor. Science 1993; 259:1742-5.
10. Robinson DS, Ying S, Taylor IK, Wangoo A, Mitchell DM, Kay AB, Hamid Q, Shaw RJ. Evidence for a Th1- like bronchoalveolar T-cell subset and predominance of interferon-gamma gene activation in pulmonary tuberculosis. Am J Respir Crit Care Med 1994; 149:989- 93.
11. Condos R, Rom WN, Liu YM, Schluger NW. Local immune responses correlate with presentation and outcome in tuberculosis. Am J Respir Crit Care Med 1998; 157:729-35.
12. Taha RA, Kotsimbos TC, Song YL, Menzies D, Hamid Q. IFN-gamma and IL-12 are increased in active compared with inactive tuberculosis. Am J Respir Crit Care Med 1997; 155:1135-9.
13. Liu X, Hou X-F, Gao L,Deng GF, Zhang MX, Deng QY, Ye TS, Yang QT, Zhou BP, Wen ZH, Liu HY, Kornfeld H, Chen XC. Indicators for prediction of Mycobacterium tuberculosis positivity detected with bronchoalveolar lavage fluid. Infectious Diseases of Poverty 2018; 7:22.
14. Küpeli E, Karnak D, Beder S, Kayacan O, Tutkak H. Diagnostic accuracy of cytokine levels (TNF-alpha, IL-2 and IFN-gamma) in bronchoalveolar lavage fluid of smear-negative pulmonary tuberculosis patients. Respiration 2008; 75(1):73-8. Epub 2007 Nov 1.
15. Taha RA, Minshall EM, Olivenstein R, Ihaku D, Wallaert B, Tsicopoulos A, Tonnel AB, Damia R, Menzies D, Hamid QA. Increased expression of IL-12 receptor mRNA in active pulmonary tuberculosis and sarcoidosis. Am J Respir Crit Care Med 1999; 160:1119-23.
16. Nolan A, Fajardo E, Huie ML, Condos R, Pooran A, Dawson R, Dheda K, Bateman E, Rom WN, Weiden MD. Increased Production of IL-4 and IL-12p40 from bronchoalveolar lavage cells are biomarkers of Mycobacterium tuberculosis in the Sputum. PLoS ONE 2013; 8(3):e59461.
17. Somoskovi A, Zissel G, Zipfel PF, Ziegenhagen MW, Klaucke J, Haas H, Schlaak M, Müller-Quernheim J. Different cytokine patterns correlate with the extension of disease in pulmonary tuberculosis. Eur Cytokine Netw 1999; 10:135-42.
18. Pushpakom R, Ong-Ajyooth S, Bovornkiti S. The association of adenosine deaminase activity with T-lymphocytes and subsets in pulmonary tuberculosis and bronchogenic carcinoma. J Med Assoc Thai 1990; 73:1-11.
19. Tchou-Wong KM, Tanabe O, Chi C, Yie TA, Rom WN. Activation of NK-kappaB in Mycobacterium tuberculosis- induced interleukin-2receptor expression in mononuclear phagocytes. Am J Respir Crit Care Med 1999; 159:1323-9.