Ratlarda Radyoterapiye Bağlı Renal Toksisitede Mesnanın Koruyucu Rolü

Abstract

Amaç: Sodyum 2-merkaptoethanosülfonat mesna alkilleyici antineoplastik ilaçlardan siklofosfamid ve ifosfomidin neden olduğu hemorajik sistiti ve nefrotoksisiteyi önlediği bilinen sentetik bir tiyoldür. Mesnanın küçük moleküler yapısı, böbrekte konsantre olma özelliği, tübüler epitelde aktif –SH bileşiklerine dönüşmesi ve antioksidan potansiyeli ile böbreklerdeki radyasyon hasarını engellemekte faydalı olabileceğini düşündük.Gereç ve Yöntemler: Bu deneysel çalışma 8 haftalık 24 Sprague-Dawley ratları ile yapılmıştır. Her grupta 6 rat olmak üzere 4 grup oluşturuldu. Grup I: Sadece serum fizyolojik uygulandı. Grup II: Sadece mesna uygulandı. Grup III: Mesna, 5 gün boyunca radyoterapiden 20 dakika önce verildi. Grup IV: İki paralel alandan 6 MV foton ile, 400 cGy'lik fraksiyonlarla toplam 20 Gy doz bilateral böbreklere uygulandı

Keywords

• Mesna, Nefrotoksisite, Radyasyon koruyucusu

The Protective Role of Mesna in Renal Toxicity Associated with Radiotherapy in Rats

Abstract

Objective: Sodium 2-mercaptoethanosulphinate mesna is a synthetic thiol known to prevent hemorrhagic cystitis and nephrotoxicity induced by alkylating anti- neoplastic drugs such as cyclophosphamide and iphosphamide. We proposed that mesna might be effective on protection of radiation induced renal injuries because of its low molecular weight, its ability to concentrate in the kidneys, its conversion to active -SH compounds in the tubular epithelium and its antioxidant potential.Material and Methods: This experimental study was carried out on 24 8-week old SpragueDawley rats. The rats were allocated into 4 groups, 6 rats in each. Group I: Only saline solution was infused. Group II: Only mesna was infused. Group III: Mesna was infused, 20 minutes before radiotherapy, for 5 days. Group IV: Radiotherapy was given bilaterally to the kidneys at a dose of 20 Gy, in 400cGy fractions, using 6 MV photon in two parallel fields.Results: There was no statistical difference between the radiotherapy and radiotherapy+mesna groups in terms of creatinine clearance p>0.05 . However; there was statistically significant difference between the control group and radiotherapy/mesna+radiotherapy in terms of blood BUN levels p0.05 .Conclusion: Our study may pioneer studies searching for other agents instead of mesna for preventing hemorrhagic cystitis induced by cyclophosphamide and iphosphamide because it showed that mesna cannot protect kidneys from radiation nephrotoxicity on histopathologic evaluation. This is also the first study demonstrating ineffectiveness of mesna as a radioprotectant

Keywords

• Mesna, Nephrotoxicity, Radioprotectant

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