Diz Osteoartrit Hastalarının Periferik Kan ve Kıkırdak Doku Örneklerinde Proinflamatuvar Sitokinlerin Etkilerinin Araştırılması

Öz

Amaç: Osteoartrit OA ağrı ve fonksiyon kısıtlılığıyla sonuçlanan dejeneratif bir eklem hastalığıdır. Metabolizmada düzenleyici moleküller olmaları ve OA patofizyolojisindeki rolleri nedeniyle sitokinler; tanı ve tedavi için araştırılan önemli faktörlerdendir. Bu çalışmanın amacı; IL-1β, IL-6, IL-17, IL-18 ve TNF-α proinflamatuvar sitokinlerinin OA ile ilişkisini göstermek ve kıkırdak dokusunda harabiyete neden olan bozulmuş katabolik aktivitelerini saptamaktır. Ayrıca bu moleküllerin kan ve kıkırdak dokularında kıyaslanarak tanı ve tedavi süreçleri için olası biyomarker adaylarının belirlenmesi amaçlanmıştır.Gereç ve Yöntemler: Hasta ve kontrol grubu olmak üzere ikiye ayrılan toplam 30 olgudan alınan periferik kan ve kıkırdak doku örneklerinde ilgili sitokinlerin protein düzeylerinin belirlenmesinde Western blot, gen ekspresyon seviyelerinin belirlenmesinde ise kantitatif gerçek zamanlı polimeraz zincir reaksiyonu yöntemleri uygulanmıştır. Ayrıca olguların C-reaktif protein CRP düzeyleri ve Kellgren-Lawrence K-L skorlamaları da sorgulanmıştır.Bulgular: Protein düzeyleri için dokuda IL-6, kanda ise IL-1β ve IL-17 istatistiksel olarak anlamlı bulunmuştur sırasıyla p

Anahtar Kelimeler

• Osteoartrit, İnflamasyon, Sitokinler, Moleküler Tıp

Investigation of the Proinflammatory Cytokines’ Effects in Peripheral Blood and Cartilage Tissue Samples of Knee Osteoarthritis Patients

Abstract

Objective: Osteoarthritis OA is a degenerative joint disease that results in pain and dysfunction. Cytokines, which are regulatory molecules in the metabolism with roles in the pathophysiology of OA, are important factors investigated regarding diagnosis and treatment. The aim of this study was to demonstrate the association between IL-1β, IL-6, IL-17, IL-18 and TNF-α proinflammatory cytokines and OA, and to identify the imbalanced catabolic activity in cartilage tissue. We also aimed to determine possible biomarker candidates for diagnosis and treatment processes by comparing cytokines in serum and cartilage Material and Methods: Western blotting was performed to determine protein levels, and quantitative real-time polymerase chain reaction was performed to determine the gene expression levels of cytokines in the blood and tissue samples taken from patient and control groups for a total of 30 cases. The C-reactive protein CRP levels and Kellgren-Lawrence K-L scoring of patients were also investigated. Results: As regards protein levels, the level of IL-6 in tissue, and of IL-1β and IL-17 in blood were found to be statistically significant p

Keywords

• Osteoarthritis, Inflammation, Cytokines, Molecular Medicine

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