The Therapeutic Efficacy of Gentisic Acid in Torsion-Detorsion Model of Ovarian Tissue in Rats

Abstract

Objective: This study aimed to investigate the therapeutic effectiveness of gentisic acid in ovarian damage caused by torsion-detorsion. Material and Methods: This research study was conducted using 32 female Wistar rats. All subjects were grouped into sham, torsion detorsion (TD), TD+Low dose and TD+High dose treatment groups in accordance with the experimental procedure. The TD model was created by clamping all of the arteries and collaterals feeding the ovary for 3 hours. Then, the clamp was opened and reperfusion was allowed for 3 hours. In TD+Low and TD+High dose treatment groups, gentisic acid was administered orally at doses of 100 and 200 mg/kg for 1 week, along with the TD model. Anesthesia conditions were created to ensure that the subjects did not feel any pain during all processes. Results: While the levels of oxidant parameters increased in the TD group compared to the Sham group, antioxidant levels were observed to decrease significantly in the TD+Low dose and TD+High dose groups. It was determined that while the levels of oxidant parameters decreased due to gentisic acid applications, antioxidant levels increased. Conclusion: Current data showed that ovarian damage caused by TD can be alleviated with low and high dose gentisic acid treatments.

Keywords

• Gentisic acid
• torsion detorsion
• ovarian tissue
• rat

The Therapeutic Efficacy of Gentisic Acid in Torsion-Detorsion Model of Ovarian Tissue in Rats

Öz

Amaç: Bu çalışmada torsiyon detorsiyon aracılığıyla gerçekleşen over hasarında gentisik asit’in tedavi edici etkinliğinin araştırılması hedeflenmiştir. Gereç ve Yöntemler: Bu araştırma çalışması, 32 adet dişi Wistar sıçan kullanılarak yapıldı. Deney prosedürüne uygun olarak tüm denekler sham, torsiyon detorsiyon (TD), TD+Düşük doz ve TD+Yüksek doz tedavi grupları olacak biçimde gruplandırıldı. TD modeli overi besleyen arter ve kolleterallerin tamamı 3 saat klemplenerek oluşturuldu. Akabinde ise klemp açılarak 3 saat boyunca reperfüzyona izin verildi. TD+Düşük ve TD+Yüksek doz tedavi grupları TD modeli ile beraber 1 hafta süreyle 100 ve 200 mg/kg dozlarında gentisik asit oral yolla uygulandı. Tüm süreçlerde deneklerin acı duymaması için anestezi koşulları oluşturuldu. Bulgular: Sham grubuna göre TD grubunda oksidan parametrelerin düzeyleri yükselirken, antioksidan düzeylerinin ise TD+Düşük doz ve TD+Yüksek doz gruplarında anlamlı düzeyde azaldığı görüldü. Gentisik asit uygulamalarına bağlı olarak oksidan parametrelerin düzeyleri azalırken, antioksidan seviyelerinin arttığı belirlendi. Sonuç: Mevcut veriler TD'nin neden olduğu over hasarının düşük ve yüksek doz gentisik asit tedavileri ile hafifletilebileceğini gösterdi.

Anahtar Kelimeler

• Gentisik asit
• torsiyon detorsiyon
• yumurtalık dokusu
• sıçan

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