Brusatol Over İskemi Reperfüzyonu ile İndüklenmiş Over Dokusu Oksidatif Yaralanmasını Azaltır

Abstract

Objective: In the scope of this study, it was tried to examine the probable benefits of brusatol against ovarian tissue injury originating from bilateral ovarian torsion/detorsion (T/D). Material and Methods: In this study, experimental animals were randomized to 4 groups. Groups were programmed as sham group I (control), group II (T/D), group III (DMSO+T/D) and group IV (Brusatol+T/D). Group I; the abdomen region was barbered, disinfected, opened by incision and closed respectively, but no T/D model was performed. Group II, as described in group I, after opening and closing abdominal incision, 3 hours of torsion was applied and it was followed by a 3-hour of detorsion. Group III, 1% DMSO was applied intraperitoneally 0,3 ml as every other day for 10 days and the last dose was given 30 minutes prior to detorsion. Group IV; brusatol was administered intraperitoneally 0,5 mg/ml once every 2 days for 10 days and the last dose was applied 30 minutes prior to detorsion. Then, all processes were carried out as described in group II. Following detorsion period, in pursuit of sacrificing the rats, ovarian tissues were removed. Results: Oxidant parameters (MDA, MPO, TOS, OSI) and pro-inflammatory cytokine (TNF-α, IL-1β) levels increased significantly while antioxidant levels (SOD, TAS) reduced in group II and III compared to group I (p<0.05). On the contrary, brusatol application (group IV) reversed all of these values compared to group II and III (p<0.05). Conclusion: As a conclusion, brusatol was evaluated to have protective effects against T/D-induced ovarian injury in rats.

Keywords

• Brusatol
• Ovary
• Torsion-Detorsion
• Ischemia Reperfusion
• Rat

Brusatol Over İskemi Reperfüzyonu ile İndüklenmiş Over Dokusu Oksidatif Yaralanmasını Azaltır

Abstract

Amaç: Bu çalışmanın kapsamında bilateral over torsiyon/detorsiyonundan (T/D) kaynaklanan over dokusu yaralanmasına karşı brusatolün olası yararları araştırılmaya çalışıldı. Gereç ve Yöntemler: Bu çalışmada deney hayvanları 4 gruba randomize edildi. Gruplar grup I (sham-kontrol), grup II (T/D), grup III (DMSO+T/D) ve grup IV (Brusatol+T/D) olarak programlandı. Grup I; karın bölgesi traşlanıp, dezenfekte edildi, kesi ile açıldı ve tekrar kapatıldı, ancak T/D modeli uygulanmadı. Grup II'de, grup I'de tarif edildiği gibi, abdominal insizyon yapıldıktan sonra 3 saat torsiyon uygulandı ve bunu 3 saatlik detorsiyon izledi. Grup III, %1 DMSO, 10 gün boyunca her gün olduğu gibi intraperitonal olarak 0,3 ml uygulandı ve son doz detorsiyondan 30 dakika önce uygulandı. Daha sonra, tüm işlemler grup II' de tarif edildiği gibi gerçekleştirildi. Grup IV; brusatol, 10 gün boyunca her 2 günde bir 0,5 mg/ml intraperitonal olarak uygulandı ve detorsiyondan 30 dakika önce son dozu verildi. Detorsiyon süresinin ardından sıçanların sakrifiye edilerek over dokuları çıkarıldı. Bulgular: Grup II ve III' te grup I ile karşılaştırıldığında, oksidan parametreler (MDA, MPO, TOS, OSI) ve proinflamatuar sitokin (TNF-α, IL-1β) düzeyleri artarken, antioksidan (SOD, TAS) parametreler önemli ölçüde azaldı (p<0.05). Aksine, brusatol uygulaması (grup IV), grup II ve III' e kıyasla bu değerlerin hepsini tersine çevirdi (p<0.05). Sonuç: Sonuç olarak, brusatol’ ün sıçanlarda T/D kaynaklı over hasarına karşı koruyucu etkileri olduğu değerlendirildi.

Keywords

• Brusatol
• Over
• Torsiyon-Detorsiyon
• İskemi Reperfüzyon
• Sıçan

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