Lazer Taramalı Konfokal Mikroskobun Prensipleri ve Tıpta Kullanım Alanları

Öz

Floresan mikroskobunun gelişimi, lazer taramalı konfokal mikroskobunun icadı ile devrim yapmıştır. Üç boyutlu gösterim ve analiz teknikleriyle bu teknoloji özellikle tıp/biyomedikal dünyasına daha gerçek bir bakış sağlamıştır. Konfokal mikroskobu, üç boyutlu yapıya sahip floresan işaretli numunelerin görüntülenmesi için bir ışık mikroskobi tekniğidir. Konfokal mikroskop uygulamaları; fikse edilmiş veya canlı hücrelerde makromoleküllerin uzamsal dağılımının görüntülenmesini, üç boyutlu verilerin toplanmasını, çoklu floresan ile işaretlenmiş örneklerin görüntülenmesini ve canlı hücrelerdeki fizyolojik olayların ölçülmesini içerir. Bu derlemede, lazer taramalı konfokal görüntülemenin prensipleri, tarihçesi, diğer mikroskoplardan ayrılan özellikleri, ayrıca yenilikçi ve önemli yönleri ve tıpta kullanım alanları sunulmaktadır.

Anahtar Kelimeler

• Lazer taramalı konfokal mikroskop
• Floresan mikroskop
• Mikroskopi

Principles of Laser Scanning Confocal Microscope and Applications in Medicine

Abstract

The development of the fluorescent microscope is revolutionized with the invention of laser scanning confocal microscopy. With its three-dimensional display and analysis techniques, this technology has provided a more real view of the medical / biomedical world. Confocal microscope is based on a light microscopic technique for imaging fluorescently labeled samples with a three-dimensional structure. Confocal microscope applications include imaging the spatial distribution of macromolecules in fixed or live cells, collecting three-dimensional data, displaying multiple fluorescently labeled samples, and measuring physiological events in living cells. In this review; the principles of the laser scanning confocal imaging, history of confocal microskopy, features distinguished from other microscopes, as well as its innovative and important aspects and applications to medicine are presented.

Keywords

• Laser scanning confocal microscope
• Fluorescence microscope
• Microscopy

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